Breeding of CMS maintainer lines through anther culture assi

发布时间：2021-06-24 07:58

　　The integrated use of molecular marker-assisted selection（MAS） and anther culture has potential to significantly increase efficiency in plant breeding; however, reports on this kind of practical use are very limited.In the present study, we report the development of cytoplasmic male sterile（CMS） maintainers with aroma, disease resistance and red-brown hulls, as an example of integration of MAS and anther culture in rice breeding.A high-resolution melting（HRM）-based functional molecular marker wa... 

【文章来源】：Journal of Integrative Agriculture. 2020,19(12)SCICSCD

【文章页数】：9 页

【文章目录】：
1. Introduction
2. Materials and methods
    2.1. Plant materials
    2.2. Genotyping by HRM-based markers
    2.3. Anther culture
    2.4. Evaluation of rice blast reaction
    2.5. Evaluation of agronomic traits and fragrance
3. Results
    3.1. Development of HRM-based marker for the red-brown hull trait and selection of homozygous rbh1,fgr and Pi2 BC2F2 plants
    3.2. Production of doubled haploid lines
    3.3. Performance of DH lines
4.Discussion
5. Conclusion


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