Mutagenesis reveals that the rice OsMPT3 gene is an importan

发布时间：2021-06-26 12:01

　　Plant mitochondrial phosphate transporters regulate phosphate transport and ATP synthesis. Determining whether they function in abiotic stress response process would shed light on their response to salt stress. We used the CRISPR/Cas9 gene-editing system to mutagenize two mitochondrial phosphate transporters, OsMPT3;1 and OsMPT3;2, to investigate their regulatory roles under salt stress. Two cas9（CRISPR-associated protein9）-free homozygous mutants, mpt33 and mpt30, were confirmed to be stable. B... 

【文章来源】：The Crop Journal. 2020,8(03)SCICSCD

【文章页数】：15 页

【文章目录】：
1. Introduction
2. Materials and methods
    2.1. Vector construction and plant transformation
    2.2. Identification of mutants
    2.3. Plant culture and treatment
    2.4. RNA isolation and PCR analyses
    2.5. Determination of ATP content in rice leaves
    2.6. Physiological measurements
    2.7. Transcriptome and metabolome sequencing
    2.8. Flux measurement of Na and K ions in roots
3. Results
    3.1. OsMPT3;1 and OsMPT3;2 are homologs
    3.2. The generated mutants show successful gene editing
    3.3. Mutants showed lower tolerance to salt stress
    3.4. Mutagenesis of the OsMPT3s genes down-regulated the expression of genes encoding ATPase under salt stress
    3.5. The mutants showed altered distributions of ATP,P,K+,Na+,and Ca2+in leaves
    3.6. The mutations affected the flux profiles of Na,K and Ca ions in rice roots
    3.7. The mutants showed high sensitivity to ATP
    3.8. The mutants trigger the alteration of the transcriptional levels
    3.9. Mutagenesis of the OsMPT3;2 gene changed the distribu-tion of the differential metabolites
4. Discussion
    4.1. Mitochondrial phosphate transporter functions by coordi-nating ATPase
    4.2. The OsMPT3 gene is an osmotic regulator depending on ATP synthesis
    4.3. OsMPT3s play important regulatory role in accumulation of metabolites
Author contributions
Appendix A.Supplementary data


【参考文献】：
期刊论文
[1]过量表达水稻OsP5CS1和OsP5CS2基因提高烟草脯氨酸的生物合成及其非生物胁迫抗性（英文）[J]. 张霞,唐维,刘嘉,刘永胜.  应用与环境生物学报. 2014(04)
[2]HPLC法测定采后莲雾果实中ATP、ADP及AMP的含量[J]. 吴光斌,陈静,陈发河.  中国食品学报. 2013(09)



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