Genetic analysis and QTL mapping of a novel reduced height g

发布时间：2021-10-26 07:12

　　Low stature in wheat is closely associated with lodging resistance, and this impacts harvest index and grain yield. The discovery of novel dwarfing or semi-dwarfing genes can have great significance for dwarf wheat breeding. In this study, we identified an EMS-induced dwarf wheat mutant JE0124 from the elite cultivar Jing411. JE0124 possesses increased stem strength and a 33% reduction in plant height compared with wild type. Gibberellic acid（GA） treatment analysis suggested that JE0124 was GA-s... 

【文章来源】：Journal of Integrative Agriculture. 2020,19(07)SCICSCD

【文章页数】：10 页

【文章目录】：
1. Introduction
2. Materials and methods
    2.1. Plant materials and growth measurement
    2.2. Cytological and histological analysis
    2.3. GA treatment
    2.4. DNA extraction and quality assessment
    2.5. Bulked segregant analysis(BSA)by using wheat660K SNP array
    2.6. Development of molecular markers by kompetitive allele-specific PCR(KASP)assays
    2.7. Linkage map construction
3. Results
    3.1. Phenotypic variations of the dwarf mutant JE0124
    3.2. Internode characteristics and GA sensitiveness
    3.3. Genetic analysis of reduced height gene in JE0124
    3.4. Mapping QTL using 660K SNP array-based BSA
    3.5. Genetic linkage map analysis of the Rht gene on chromosome 2DL
4. Discussion
5. Conclusion


【参考文献】：
期刊论文
[1]用STS标记检测矮秆基因Rht-B1b和Rht-D1b在中国小麦中的分布[J]. 杨松杰,张晓科,何中虎,夏先春,周阳.  中国农业科学. 2006(08)



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