Functional Analysis of Phosphate Transporter OsPHT4 Family M

发布时间：2021-12-30 23:23

　　The transport of phosphate between cytoplasm and subcellular compartments is critical for plant metabolic regulation. We conducted bioinformatic analysis, heterologous expression in yeast, gene expression pattern and subcellular localization analysis to characterize the possible functions of OsPHT4 gene family in rice. Together with the PHT4 genes from higher plants, OsPHT4 s can be classified into six distinct groups. OsPHT4;1–OsPHT4;4 are targeted to chloroplasts, and OsPHT4;6-1 and OsPHT4;6-2... 

【文章来源】：Rice Science. 2020,27(06)SCICSCD

【文章页数】：11 页

【文章目录】：
MATERIALS AND METHODS
    Bioinformatic and phylogenetic analysis of Os PHT4gene family
    Plant materials and growth conditions
    RNA isolation,quantification and first-strand c DNA synthesis
    Quantitative real-time PCR (q RT-PCR)
    Subcellular localization of OsPHT4 in rice protoplasts
    Complementation of yeast Pi transport mutant by PHT4 genes
    Analysis of promoters of Os PHT4 family genes
    Accession numbers of genes used
RESULTS
    Phylogenetic analysis of Os PHT4 gene family
    Expression patterns of Os PHT4 genes in rice
    Subcellular localization of OsPHT4 family members
    Pi transport activity of OsPHT4 genes in yeast
    Expression patterns of Os PHT4 genes in response to phosphate deprivation
    Expression patterns of Os PHT4 genes in response to salt,ABA and SA treatments
DISCUSSION
SUPPLEMENTAL DATA


【参考文献】：
期刊论文
[1]水稻磷转运蛋白OsPHT2;1在提高磷素利用率方面的作用[J]. 史书林,王丹凤,颜彦,张芳,王化敦,顾冕,孙淑斌,徐国华.  中国水稻科学. 2013(05)



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