水稻苗期缺钾条件下根数QTL qRN5a的精细定位
发布时间：2022-01-17 10:47
钾（K）是植物生长发育等多种生理过程中不可或缺的矿物质成分。水稻缺K现象常常发生,导致生长受限和减产。目前,我们对水稻低K（LK）耐受的分子机制仍然知之甚少。本研究通过构建遗传群体,对水稻苗期缺钾条件下根数QTL qRN5a进行了精细定位及候选基因分析。研究结果如下:1.利用中恢9308（ZH9308,LK敏感型）和协青早B（XQZB,LK耐受型）杂交配组而成的75个染色体片段代换系（CSSLs）对苗期地上部和根部性状进行了QTL分析。在水培条件下,研究了5个苗期性状（根长、根数、根干重、茎干重和总干重）在LK和正常K（NK）条件下的QTL及其相关性状的比值（LK/NK）。在4条染色体（3、4、5和6）上共鉴定到5个QTL,XQZB对根长（RL）、根数（RN）具有正等位效应,对地上部干重（SDW）、根干重（RDW）具有负等位效应。在LK条件下检测到2个QTL,qRN5a和qSDW4,在LK/NK条件下鉴定到3个QTL,qRL6、qRN5b和qRDW3,解释了11.81%至13.07%的总表型变异。在已鉴定的QTL中,第5染色体上与根数相关的QTL qRN5a和qRN5b最有效,因为这些...
【文章来源】：中国农业科学院北京市
【文章页数】：103 页
【学位级别】：博士
【文章目录】：
摘要
abstract
CHAPTER1 INTRODUCTION
1.1 GENERAL INTRODUCTION
1.2 REVIEW OF LITERATURE
1.2.1 Availability of potassium and its requirements
1.2.2 Functions of potassium in the plant
1.2.3 Potassium transporter and its functions in plants
1.2.4 Plant sensing and signaling with regards to potassium
1.2.5 Adaptation to potassium deficiency and syndromes
1.2.6 Root response associated with potassium
1.2.7 Quantitative trait locus(QTL)mapping and its genetic basis
1.2.8 QTL for mineral nutrient potassium(K)
1.2.9 A brief of mapping population for QTL analysis
1.3 PROSPECT OF K DEFICIENCY TOLERANT QTL
CHAPTER2 IDENTIFICATION OF QTL FOR RICE SEEDLING TRAITS USING CSSL POPULATION UNDER DIFFERENT POTASSIUM LEVELS
2.1 INTRODUCTION
2.2 MATERIALS AND METHODS
2.2.1 Development of the CSSL population
2.2.2 Hydroponic plant cultivation and phenotypic evaluation
2.2.3 Sampling and measurement of seedling traits
2.2.4 DNA extraction for genotyping
2.2.5 Data analysis and QTL Mapping
2.3 RESULTS
2.3.1 Phenotypic variation in CSSL population
2.3.2 Correlation coefficients for studied traits
2.3.3 QTL analysis for seedling traits
2.4 DISCUSSION
2.5 CONCLUSIONS
CHAPTER3 FINE MAPPING AND CANDIDATE GENE ANALYSIS OF qRN5a,A QTL UNDER LOW K THAT POSITIVELY REGULATES ROOT NUMBER AT THE SEEDLING STAGE IN RICE
3.1 INTRODUCTION
3.2 METHODS AND MATERIALS
3.2.1 Plant materials
3.2.2 Hydroponic culture conditions
3.2.3 Sampling and measurement of seedling root traits
3.2.4 DNA extraction and molecular marker development
3.2.5 RNA extraction and quantitative real-time(qRT)PCR
3.2.6 Data analysis
3.3 RESULTS
3.3.1 Phenotypic evaluation of the F2(BC5F2)population with parents
3.3.2 Validation and delimitation of qRN5a using the F2(BC5F2)population
3.3.3 Phenotypic evaluation of qRN5a
3.3.4 Fine mapping of qRN5a
3.3.5 Candidate genes analysis of qRN5a
3.3.6 Expression pattern analysis of candidate genes of qRN5a
3.3.7 Relation between qRN5a and other root growth-regulating genes and K transporters
3.4 DISCUSSION
3.4.1 qRN5a is the first fine mapped QTL controlling RN under LK in rice
3.4.2 qRN5a is involved in the root development regulatory network under LK
3.5 CONCLUSION
CHAPTER4 MAJOR FINDINGS AND FUTURE PERSPECTIVE
4.1 MAJOR FINDINGS
4.2 FUTURE PERSPECTIVE
REFERENCES
APPENDIX A
ACKNOWLEDGMENTS
AUTHOR’S RESUME
【参考文献】：
期刊论文
[1]水稻耐低钾种质资源的苗期筛选[J]. 王广洋,陆文怡,陈慧男,张晓勤,薛大伟. 杭州师范大学学报(自然科学版). 2015(01)
[2]Transport, signaling, and homeostasis of potassium and sodium in plants[J]. Eri Adams,Ryoung Shin. Journal of Integrative Plant Biology. 2014(03)
[3]Molecular evolution and functional divergence of HAK potassium transporter gene family in rice（Oryza sativa L.）[J]. Zefeng Yang a,Qingsong Gao a,Changsen Sun b,Wenjuan Li a,Shiliang Gu a,Chenwu Xu a,a Jiangsu Provincial Key laboratory of Crop Genetics and Physiology,Key Laboratory of Plant Functional Genomics of Ministry of Education,Yangzhou University,Yangzhou 225009,China b School of Life Sciences,Taizhou University,Linhai 317000,China. 遗传学报. 2009(03)
[4]数量性状基因的完备区间作图方法[J]. 王建康. 作物学报. 2009(02)
[5]Membrane Transporters for Nitrogen,Phosphate and Potassium Uptake in Plants[J]. Yi-Fang Chen, Yi Wang and Wei-Hua Wu (State Key Laboratory of Plant Physiology and Biochemistry, College of Biological Sciences, China Agricultural University, National Plant Gene Research Centre, Beijing 100094, China). Journal of Integrative Plant Biology. 2008(07)
[6]Differential response of root morphology to potassium deficient stress among rice genotypes varying in potassium efficiency[J]. Ghulam JILANI. Journal of Zhejiang University（Science B:An International Biomedicine & Biotechnology Journal）. 2008(05)
[7]The role of calcium sensor-interacting protein kinases in plant adaptation to potassium-deficiency: new answers to old questions[J]. Anna Amtmann,Patrick Armengaud. Cell Research. 2007(06)
[8]低钾胁迫下水稻钾高效基因型若干生长特性和营养特性的研究[J]. 刘建祥,杨肖娥,杨玉爱,吴良欢. 植物营养与肥料学报. 2003(02)
[9]籼稻耐低钾基因型的筛选[J]. 刘国栋,刘更另. 作物学报. 2002(02)
[10]高质量植物基因组DNA的分离[J]. 罗志勇,周钢,陈湘晖,陆秋恒,胡维新. 湖南医科大学学报. 2001(02)
本文编号：3594591
【文章来源】：中国农业科学院北京市
【文章页数】：103 页
【学位级别】：博士
【文章目录】：
摘要
abstract
CHAPTER1 INTRODUCTION
1.1 GENERAL INTRODUCTION
1.2 REVIEW OF LITERATURE
1.2.1 Availability of potassium and its requirements
1.2.2 Functions of potassium in the plant
1.2.3 Potassium transporter and its functions in plants
1.2.4 Plant sensing and signaling with regards to potassium
1.2.5 Adaptation to potassium deficiency and syndromes
1.2.6 Root response associated with potassium
1.2.7 Quantitative trait locus(QTL)mapping and its genetic basis
1.2.8 QTL for mineral nutrient potassium(K)
1.2.9 A brief of mapping population for QTL analysis
1.3 PROSPECT OF K DEFICIENCY TOLERANT QTL
CHAPTER2 IDENTIFICATION OF QTL FOR RICE SEEDLING TRAITS USING CSSL POPULATION UNDER DIFFERENT POTASSIUM LEVELS
2.1 INTRODUCTION
2.2 MATERIALS AND METHODS
2.2.1 Development of the CSSL population
2.2.2 Hydroponic plant cultivation and phenotypic evaluation
2.2.3 Sampling and measurement of seedling traits
2.2.4 DNA extraction for genotyping
2.2.5 Data analysis and QTL Mapping
2.3 RESULTS
2.3.1 Phenotypic variation in CSSL population
2.3.2 Correlation coefficients for studied traits
2.3.3 QTL analysis for seedling traits
2.4 DISCUSSION
2.5 CONCLUSIONS
CHAPTER3 FINE MAPPING AND CANDIDATE GENE ANALYSIS OF qRN5a,A QTL UNDER LOW K THAT POSITIVELY REGULATES ROOT NUMBER AT THE SEEDLING STAGE IN RICE
3.1 INTRODUCTION
3.2 METHODS AND MATERIALS
3.2.1 Plant materials
3.2.2 Hydroponic culture conditions
3.2.3 Sampling and measurement of seedling root traits
3.2.4 DNA extraction and molecular marker development
3.2.5 RNA extraction and quantitative real-time(qRT)PCR
3.2.6 Data analysis
3.3 RESULTS
3.3.1 Phenotypic evaluation of the F2(BC5F2)population with parents
3.3.2 Validation and delimitation of qRN5a using the F2(BC5F2)population
3.3.3 Phenotypic evaluation of qRN5a
3.3.4 Fine mapping of qRN5a
3.3.5 Candidate genes analysis of qRN5a
3.3.6 Expression pattern analysis of candidate genes of qRN5a
3.3.7 Relation between qRN5a and other root growth-regulating genes and K transporters
3.4 DISCUSSION
3.4.1 qRN5a is the first fine mapped QTL controlling RN under LK in rice
3.4.2 qRN5a is involved in the root development regulatory network under LK
3.5 CONCLUSION
CHAPTER4 MAJOR FINDINGS AND FUTURE PERSPECTIVE
4.1 MAJOR FINDINGS
4.2 FUTURE PERSPECTIVE
REFERENCES
APPENDIX A
ACKNOWLEDGMENTS
AUTHOR’S RESUME
【参考文献】：
期刊论文
[1]水稻耐低钾种质资源的苗期筛选[J]. 王广洋,陆文怡,陈慧男,张晓勤,薛大伟. 杭州师范大学学报(自然科学版). 2015(01)
[2]Transport, signaling, and homeostasis of potassium and sodium in plants[J]. Eri Adams,Ryoung Shin. Journal of Integrative Plant Biology. 2014(03)
[3]Molecular evolution and functional divergence of HAK potassium transporter gene family in rice（Oryza sativa L.）[J]. Zefeng Yang a,Qingsong Gao a,Changsen Sun b,Wenjuan Li a,Shiliang Gu a,Chenwu Xu a,a Jiangsu Provincial Key laboratory of Crop Genetics and Physiology,Key Laboratory of Plant Functional Genomics of Ministry of Education,Yangzhou University,Yangzhou 225009,China b School of Life Sciences,Taizhou University,Linhai 317000,China. 遗传学报. 2009(03)
[4]数量性状基因的完备区间作图方法[J]. 王建康. 作物学报. 2009(02)
[5]Membrane Transporters for Nitrogen,Phosphate and Potassium Uptake in Plants[J]. Yi-Fang Chen, Yi Wang and Wei-Hua Wu (State Key Laboratory of Plant Physiology and Biochemistry, College of Biological Sciences, China Agricultural University, National Plant Gene Research Centre, Beijing 100094, China). Journal of Integrative Plant Biology. 2008(07)
[6]Differential response of root morphology to potassium deficient stress among rice genotypes varying in potassium efficiency[J]. Ghulam JILANI. Journal of Zhejiang University（Science B:An International Biomedicine & Biotechnology Journal）. 2008(05)
[7]The role of calcium sensor-interacting protein kinases in plant adaptation to potassium-deficiency: new answers to old questions[J]. Anna Amtmann,Patrick Armengaud. Cell Research. 2007(06)
[8]低钾胁迫下水稻钾高效基因型若干生长特性和营养特性的研究[J]. 刘建祥,杨肖娥,杨玉爱,吴良欢. 植物营养与肥料学报. 2003(02)
[9]籼稻耐低钾基因型的筛选[J]. 刘国栋,刘更另. 作物学报. 2002(02)
[10]高质量植物基因组DNA的分离[J]. 罗志勇,周钢,陈湘晖,陆秋恒,胡维新. 湖南医科大学学报. 2001(02)
本文编号：3594591
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