CRISPR/Cas9-mediated disruption of TaNP1 genes results in co

发布时间：2022-08-13 13:18

　　Male sterile genes and mutants are valuable resources in hybrid seed production for monoclinous crops.High genetic redundancy due to allohexaploidy makes it difficult to obtain the nuclear recessive male sterile mutants through spontaneous mutation or chemical or physical mutagenesis methods in wheat.The emerging effective genome editing tool,CRISPR/Cas9 system,makes it possible to achieve simultaneous mutagenesis in multiple homoeoalleles.To improve the genome modification efficiency of the CRI... 

【文章页数】：10 页

【文章目录】：
1. Introduction
2. Results
    2.1. Ta NP1 genes are preferentially expressed in the tapetum
    2.2. Optimization of the CRISPR/Cas9 system in wheat protoplasts
    2.3. CRISPR/Cas9-mediated mutagenesis of Ta NP1 genes
    2.4. Triple homozygous mutations in Ta NP1 genes result in complete male sterility
    2.5. Any WT copy of the three Ta NP1 genes is sufficient for maintenance of male fertility
3. Discussion
4. Materials and methods
    4.1. Plant materials and growth condition
    4.2. RT-PCR,q RT-PCR,and in situ hybridization
    4.3. Plasmid construction
    4.4. Wheat protoplast isolation and transformation
    4.5. Biolistic-mediated transformation of wheat
    4.6. PCR/RE assay


【参考文献】：
期刊论文
[1]Research and application of hybrid wheat in China[J]. Zhao Changping.  Engineering Sciences. 2013(05)
[2]C49S温光型两系杂种小麦在江汉平原生产应用中的几个问题[J]. 陈功海,龚德平,郭西陵,邱文兵,何庆虎.  麦类作物学报. 2005(04)
[3]普通小麦D~2型CMS系的育性基因及其遗传特性[J]. 刘春光,侯宁,刘根齐,吴郁文,张翠兰,张炎.  遗传学报. 2002(07)



本文编号：3677105