The leaf extract of crofton weed(Eupatorium adenophorum)inhi

发布时间：2023-04-01 22:46

　　The extract of crofton weed(Eupatorium adenophorum) inhibits seed germination and weed growth;however,the physiological mechanisms underlying the effect of crofton weed extract on the modulation of seedling growth and root system development remain largely unclear.In this study,we investigated the effects of the leaf extract of crofton weed(LECW) on primary root(PR) growth in maize seedlings.Treatment with LECW markedly inhibited seed germination and seedling growth in a dose-dependent manner.Ph...

【文章页数】：7 页

【文章目录】：
1. Introduction
2. Materials and methods
    2.1. Preparation of the leaf extract of crofton weed
    2.2. Plant materials and growth conditions
    2.3. Detection of the accumulation of reactive oxygen species in roots
    2.4. Observation of root border cells
    2.5. Hoechst 33258 staining
    2.6. Scanning electron microscopy analysis
    2.7. Determination of mucigel thickness
    2.8. Determination of pectin methyl esterase activity
3. Results
    3.1. The leaf extract of crofton weed markedly inhibited seed germination and seedling growth
    3.2. The leaf extract of crofton weed induced reactive oxygenspecies accumulation in root tips and cell death in root border cells
    3.3. The leaf extract of crofton weed disrupted root cell structure
    3.4. The leaf extract of crofton weed reduced pectin methyl esterase activity in root caps,whereas it increased the thickness of themucilage layer of root border cells
4. Discussion
Author contributions
Declaration of Competing Interest



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