Identification of late-stage pollen-specific promoters for c

发布时间：2023-05-31 02:28

　　Large-scale production of male sterile seeds can be achieved by introducing a fertility-restoration gene linked with a pollen-killer gene into a recessive male sterile mutant. We attempted to construct this system in rice by using a late-stage pollen-specific(LSP) promoter driving the expression of maize α-amylase gene ZM-AA1.To obtain such promoters in rice, we conducted comparative RNA-seq analysis of mature pollen with meiosis anther, and compared this with the transcriptomic data of various ...

【文章页数】：18 页

【文章目录】：
INTRODUCTION
RESULTS
    Identification of genes differentially expressed in late‐stage pollen
    Analysis of late‐stage pollen‐specific cis‐element sequences in the promoters of candidate OsLSP genes
    Validation of candidate OsLSP genes by RT‐PCR and quantitative PCR
    Analysis of promoter:GFP reporters in rice
    Development of a pollen disruption system
    Identification of additional OsLSP gene promoters suitable for pollen disruption
DISCUSSION
MATERIALS AND METHODS
    Plant materials and growth conditions
    Pollen isolation
    RNA extraction and qPCR
    RNA‐seq analysis
    Analysis of late‐stage pollen‐specific cis‐elements
    Construction of plasmids
    Production of transgenic rice
    Phenotype analysis
    Accession numbers
AUTHOR CONTRIBUTIONS
SUPPORTING INFORMATION



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