QTL Mapping for Microtuber Dormancy, GA 3 and ABA Content in

发布时间：2023-12-10 14:15

　　休眠是各植物物种极其重要的现象,短暂的休眠是一个普遍问题,特别是在储存期间。在大田环境下,不统一地发芽所导致的过长休眠也一直是个悬而未决的问题。目前,管理休眠的水平已得到提升。随着对休眠复杂分子机制认识的深入,通过应用遗传学的策略,可以提供新的方法将所需的休眠期水平引入到植物物种中。然而,人们对大多数植物物种休眠的遗传调控机制仍知之甚少。休眠是马铃薯块茎储存时的显著特征。通过体外生产块茎的微块茎系统,在20℃条件下储存,对具有178个单株的F1群体的休眠期,赤霉酸(GA3)和脱落酸(ABA)含量进行了数量性状位点(QTL)分析,该群体由长休眠野生种S.chacoense acc.PI 320285和短休眠栽培种S.phureja acc.DM1-3 516 R44种间杂交所得。在此分析中,174个标记用于构建总长591.8 c M的遗传图谱。QTL分析确定了22个标记;PGSC21436₁60与休眠期和GA3含量的相关性最密切。该等位基因位点来自父本的第1号染色体,可促进长休眠,具有最大效应值,解释了9.4%的表型变异。令人惊奇的是,调控休眠和GA3的QTL位于同...

【文章页数】：118 页

【学位级别】：博士

【文章目录】：
ABSTRACT
摘要
LIST OF ABBREVIATIONS
1.INTRODUCTION
    1.1 PHYSIOLOGICAL ACTIVITY DURING DORMANCY
        1.1.1 Respiration
        1.1.2 Accumulation of reducing sugar
        1.1.3 Amino acids, nucleic acids and proteins
        1.1.4 Sub-cellular compartment
    1.2 EFFECT OF PHYTOHORMONES
        1.2.1 Abscisic Acid
        1.2.2 Ethylene
        1.2.3 Auxins
        1.2.4 Cytokinins
        1.2.5 Jasmonates
        1.2.6 Gibberellins
        1.2.7 Current model of hormonal regulation
    1.3 EFFECT OF STORAGE ON DORMANCY
        1.3.1 Light
        1.3.2 Temperature
        1.3.3 Humidity
        1.3.4 Concentrations of Carbon dioxide and oxygen
    1.4 BIO-TECHNOLOGICAL APPROACH TO CONTROL THE DORMANCY
        1.4.1 Expressed sequence tags (ESTs)
        1.4.2 cDNA-AFLP fingerprinting
        1.4.3 Potato EST libraries Construction
        1.4.4 Suppression Subtractive Hybridisation (SSH)
        1.4.5 QTL analysis
    1.5 RESEARCH OBJECTIVE
2.MATERIALS AND METHODS
    2.1 Plant material
        2.1.1 Mapping population
        2.1.2 Microtuberization
    2.2 Phenotyping
        2.2.1 Measuring dormancy period
        2.2.2 GA₃ and ABA Assay
    2.3 CTAB method to extract genomic DNA
    2.4 Marker Generation
    2.5 PCR Reactions
    2.6 Procedure for Acrylamide Gel Running
    2.7 Paternal Linkage map construction & QTL analysis
3.GENETIC LINKAGE MAP CONSTRUCTION
    3.1 Genetic linkage map construction
        3.1.1 Primer polymorphism screening
        3.1.2 Paternal linkage map construction
4.QTL MAPPING FOR MICROTUBER DORMANCY
    4.1 Introduction
    4.2 Phenotypic variation
    4.3 QTL Mapping
    4.4 Discussion
    4.5 Conclusion
5.QTL MAPPING FOR MICROTUBER GA₃ CONTENT
    5.1 Introduction
    5.2 Phenotypic variation
    5.3 QTL Mapping
    5.4 Discussion
    5.5 Conclusion
6.QTL MAPPING FOR MICROTUBER ABA CONTENT
    6.1 Introduction
    6.2 Phenotypic variation
    6.3 QTL Mapping
    6.4 Discussion
    6.5 Conclusion
7.GENERAL DISCUSSION
    7.1 GA₃ and ABA interplay role in the Dormancy Regulation
        7.1.1 GA₃: dormancy releasing hormone, endorse germination andneutralize effects of ABA
        7.1.2 ABA: regulate dormancy initiation positively, a negative controllerof sprouting
    7.2 GA₃ and dormancy correlation in the dormancy regulation
    7.3 ABA and dormancy correlation in the dormancy regulation
8.FUTURE PERSPECTIVES
9.CONCLUSIONS
LITERATURE CITED
Appendix
ACKNOWLEDGEMENTS
ABOUT THE AUTHOR



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