ND-FISH-positive oligonucleotide probes for detecting specif

发布时间：2025-01-17 10:16

　　 Rye(Secale cereale L.) has been widely used to improve wheat(Triticum aestivum L.) cultivars.Oligo probes combined with non-denaturing fluorescence in situ hybridization(ND-FISH)technology provide a convenient and efficient way to identify individual rye chromosomes.However, suitable ND-FISH-positive oligo probes for recognizing specific segments of rye chromosomes are lacking. Five new ND-FISH-positive oligo probes: Oligo-5 BL.46, Oligo-5 A8080, Oligo-5 A8080.1, Oligo-1 AL.73, and Oligo-0 R3, c...

【文章页数】：11 页

【文章目录】：
1. Introduction
2. Materials and methods
    2.1. Plant materials
    2.2. Development of oligo probes
    2.3. ND-FISH
    2.4. Similarity searches of the original sequences of probes
3. Results
    3.1. Oligo probes generating signals on rye chromosomes
    3.2. Breakpoints of 4RLKuarms in the four wheat-rye 4RLKu translocation chromosomes
    3.3. The signal patterns of seven oligo probes on wheat chromosomes
    3.4. Similar sequences with the original consensus sequences of oligo probes
4. Discussion
    4.1. Rye chromosomal segment-specific oligo probes
    4.2. Breakpoints in wheat-alien translocation chromosomes determined by FISH map
    4.3. Tandem repeats in rye
    4.4. Why the tandem repeats in wheat could produce FISH signals on rye chromosomes
    4.5. Probes Oligo-5A8080 and Oligo-5A8080.1 reflect different distributions of 5S rDNA sequences
5. Conclusion



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