日粮中叶酸对团头鲂生长以及急性高温应激下生理生化反应的影响
发布时间：2020-12-22 05:53
本试验研究了日粮中的叶酸对团头鲂(Megalobramaamblycephala)生长、生理生化以及急性高温应激下生理生化的影响。团头鲂初体重27.0±0.03g,试验配置6组半纯合日粮,分别含叶酸0.0、0.5、1.0、2.0、5.0和10.0mg/kg饲料,养殖试验在循环水系统中进行,周期为10周,每组饲料3个平行。研究结果显示,饲喂1.0、2.0和5.0 mg/kg叶酸组团头鲂的终末重、增重率和特定生长率显著高于其他两组(P<0.05)。另外,1.0mg/kg组的显著影响团头鲂的饵料系数和肥满度。饲喂1.0mg/kg和2.0mg/kg叶酸组的团头鲂的肝体比和脏体比显著增加。叶酸对团头鲂肌肉水分、(?)分和粗脂肪含量影响不显著(P>0.05)。但是显著影响肌肉粗蛋白含量,1.0mg/kg和2.0mg/kg叶酸组的团头鲂的肌肉粗蛋白含量显著高于对照组和0.5mg/kg叶酸组(P<0.05),但与其他各组差异不显著。根据增重率、特定生长率和肝体比,团头鲂日粮中最适叶酸含量分别为0.68mg/kg、0.68mg/kg和0.82mg/kg。日粮中的叶酸含量对团头鲂血液中的...
【文章来源】：南京农业大学江苏省 211工程院校 教育部直属院校
【文章页数】：91 页
【学位级别】：硕士
【文章目录】：
ABSTRACT
摘要
CHAPTER 1 Literature Review and Study Plan
1.1 Dietary folic acid
1.1.1 Functions of Folic Acid
1.2 Fish Specific Stressors
1.2.1 Capture, Transport, and Handling
1.2.2 Contaminants
1.2.3 Crowding
1.3 Stress Responses and organ system
1.3.1 Gills
1.3.2 Liver
1.3.3 Integument
1.4 An overview of the nutrition of M. amblycephala
1.5 Aim of the study
1.6 Hypothesis of the study
CHAPTER 2 Effect of dietary Folic acid on growth performance and muscle chemical composition ofblunt snout bream M. amblycephala fingerling
1 Introduction
2 Materials and Methods
2.1 Formulation and preparation of experimental diets
2.2 Research facility and fish rearing
2.3 Sampling and fish weighing
2.4 Calculations
2.5 Proximate composition analysis
2.6 Statistical analysis
3 Results
3.1 Growth performance and feed utilization
3.2 Effect of dietary folic acid level on the muscle proximate chemical composition
4 Discussion
4.1 Growth performance and feed utilization
4.2 Effect of dietary folic acid level on the muscle proximate chemical composition
5 Conclusion
CHAPTER 3 Effect of dietary Folic acid on blood hematological parameters and Digestive enzymesactivities of blunt snout bream fingerling M. amblycephala
1 Introduction
2 Materials and Method
2.1 Formulation and preparation of experimental diets
2.2 Research facility and fish rearing
2.3 Sample collection
2.4 Effect of dietary folic acid on Hematological measurements
2.5 Effect of dietary folic acid on digestive enzyme activity
2.6 Statistical analysis
3 Results
3.1 Effect of dietary folic acid on blood Hematological parameters
3.2 Effect of dietary folic acid on digestive enzyme activity
4 Discussion
4.1 Effect of dietary folic acid on blood hematology
4.2 Effect of dietary folic acid on digestive enzyme activity
5 Conclusion
CHAPTER 4 Effect of dietary folic acid on biochemical parameters, Antioxidant enzymes activities andgene expression of three heat shock proteins （HSPs） in blunt snout bream Megalobrama amblycephalafingerling under acute high temperature stress
1 Introduction
2 Materials and Method
2.1 Formulation and preparation of experimental diets
2.2 Research facility and fish rearing
2.3 Collection of Sample
2.4 Biochemical measurements
2.5 Immune and antioxidant parameters
2.6 Real-time PCR analysis of hepatic HSP60, HSP70 and HSP90
2.7 Statistical analysis
3 Results
3.1 Effect of dietary folic acid level on the serum TP, TC, TG and Urea under acute hightemperature challenge
3.2 Effect of dietary folic acid level on the serum Cortisol, Glucose, ACTH, ALT, AST andALP under acute high temperature challenge
3.3 Effect of dietary folic acid level on the serum T3 and T4 under acute high temperaturechallenge
3.4 Effect of dietary folic acid level on the serum C3,C4 and IgM under acute hightemperature challenge
3.5 Effect of dietary folic on hepatic anti-oxidization enzyme activity under acute hightemperature challenge
3.6 Effect of dietary folic acid on gene expression of three heat shock proteins （HSPs） underacute high temperature stress
4 Discussion
4.1 Effect of dietary folic acid level on the serum TP, TC, TG and Urea under acute hightemperature challenge
4.2 Effect of dietary folic acid level on the serum Glucose, Cortisol, ACTH, ALT, AST andALP under acute high temperature challenge
4.3 Effect of dietary folic acid level on the serum T3 and T4 under acute high temperatureStress
4.4 Effect of dietary folic acid level on the serum C3,C4 and IgM under acute hightemperature challenge
4.5 Effect of dietary folic on hepatic anti-oxidization enzyme activity under acute hightemperature challenge
4.6 Effect of dietary folic acid on gene expression of three heat shock proteins （HSPs） underacute high temperature stress
5 Conclusion
Conclusion of the whole study
References
Appendix
Acknowledgements
Meetings attended and publications
Awards
【参考文献】：
期刊论文
[1]叶酸的提取及抗氧化性研究[J]. 迟晓星,张涛,赵静,冯江北. 现代食品科技. 2011(10)
[2]低温胁迫对鲤血液学和血清生化指标的影响[J]. 常玉梅,匡友谊,曹鼎臣,梁利群,孙效文,雷清泉. 水产学报. 2006(05)
[3]中国对虾血淋巴蛋白质、葡萄糖含量的研究[J]. 罗日祥. 海洋与湖沼. 1996(05)
[4]一种优良淡水鱼——团头鲂(Megalobrama amblycephala)的繁殖和饲养[J]. 柯鸿文. 水生生物学集刊. 1975(03)
本文编号：2931254
【文章来源】：南京农业大学江苏省 211工程院校 教育部直属院校
【文章页数】：91 页
【学位级别】：硕士
【文章目录】：
ABSTRACT
摘要
CHAPTER 1 Literature Review and Study Plan
1.1 Dietary folic acid
1.1.1 Functions of Folic Acid
1.2 Fish Specific Stressors
1.2.1 Capture, Transport, and Handling
1.2.2 Contaminants
1.2.3 Crowding
1.3 Stress Responses and organ system
1.3.1 Gills
1.3.2 Liver
1.3.3 Integument
1.4 An overview of the nutrition of M. amblycephala
1.5 Aim of the study
1.6 Hypothesis of the study
CHAPTER 2 Effect of dietary Folic acid on growth performance and muscle chemical composition ofblunt snout bream M. amblycephala fingerling
1 Introduction
2 Materials and Methods
2.1 Formulation and preparation of experimental diets
2.2 Research facility and fish rearing
2.3 Sampling and fish weighing
2.4 Calculations
2.5 Proximate composition analysis
2.6 Statistical analysis
3 Results
3.1 Growth performance and feed utilization
3.2 Effect of dietary folic acid level on the muscle proximate chemical composition
4 Discussion
4.1 Growth performance and feed utilization
4.2 Effect of dietary folic acid level on the muscle proximate chemical composition
5 Conclusion
CHAPTER 3 Effect of dietary Folic acid on blood hematological parameters and Digestive enzymesactivities of blunt snout bream fingerling M. amblycephala
1 Introduction
2 Materials and Method
2.1 Formulation and preparation of experimental diets
2.2 Research facility and fish rearing
2.3 Sample collection
2.4 Effect of dietary folic acid on Hematological measurements
2.5 Effect of dietary folic acid on digestive enzyme activity
2.6 Statistical analysis
3 Results
3.1 Effect of dietary folic acid on blood Hematological parameters
3.2 Effect of dietary folic acid on digestive enzyme activity
4 Discussion
4.1 Effect of dietary folic acid on blood hematology
4.2 Effect of dietary folic acid on digestive enzyme activity
5 Conclusion
CHAPTER 4 Effect of dietary folic acid on biochemical parameters, Antioxidant enzymes activities andgene expression of three heat shock proteins （HSPs） in blunt snout bream Megalobrama amblycephalafingerling under acute high temperature stress
1 Introduction
2 Materials and Method
2.1 Formulation and preparation of experimental diets
2.2 Research facility and fish rearing
2.3 Collection of Sample
2.4 Biochemical measurements
2.5 Immune and antioxidant parameters
2.6 Real-time PCR analysis of hepatic HSP60, HSP70 and HSP90
2.7 Statistical analysis
3 Results
3.1 Effect of dietary folic acid level on the serum TP, TC, TG and Urea under acute hightemperature challenge
3.2 Effect of dietary folic acid level on the serum Cortisol, Glucose, ACTH, ALT, AST andALP under acute high temperature challenge
3.3 Effect of dietary folic acid level on the serum T3 and T4 under acute high temperaturechallenge
3.4 Effect of dietary folic acid level on the serum C3,C4 and IgM under acute hightemperature challenge
3.5 Effect of dietary folic on hepatic anti-oxidization enzyme activity under acute hightemperature challenge
3.6 Effect of dietary folic acid on gene expression of three heat shock proteins （HSPs） underacute high temperature stress
4 Discussion
4.1 Effect of dietary folic acid level on the serum TP, TC, TG and Urea under acute hightemperature challenge
4.2 Effect of dietary folic acid level on the serum Glucose, Cortisol, ACTH, ALT, AST andALP under acute high temperature challenge
4.3 Effect of dietary folic acid level on the serum T3 and T4 under acute high temperatureStress
4.4 Effect of dietary folic acid level on the serum C3,C4 and IgM under acute hightemperature challenge
4.5 Effect of dietary folic on hepatic anti-oxidization enzyme activity under acute hightemperature challenge
4.6 Effect of dietary folic acid on gene expression of three heat shock proteins （HSPs） underacute high temperature stress
5 Conclusion
Conclusion of the whole study
References
Appendix
Acknowledgements
Meetings attended and publications
Awards
【参考文献】：
期刊论文
[1]叶酸的提取及抗氧化性研究[J]. 迟晓星,张涛,赵静,冯江北. 现代食品科技. 2011(10)
[2]低温胁迫对鲤血液学和血清生化指标的影响[J]. 常玉梅,匡友谊,曹鼎臣,梁利群,孙效文,雷清泉. 水产学报. 2006(05)
[3]中国对虾血淋巴蛋白质、葡萄糖含量的研究[J]. 罗日祥. 海洋与湖沼. 1996(05)
[4]一种优良淡水鱼——团头鲂(Megalobrama amblycephala)的繁殖和饲养[J]. 柯鸿文. 水生生物学集刊. 1975(03)
本文编号：2931254
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