Expression Pattern of Dmrt1Gene and the Effects of Octylphen

发布时间：2021-11-24 08:15

　　本研究克隆了中国林蛙（Rana Chensinensis） Dmrtl （double-sex and mab-3related transcription factor1）基因,并检测了该基因在中国林蛙成体不同组织的表达以及在精巢中的定位。然后,将林蛙暴露于不同浓度的辛基酚（octylphenol, OP,10-8,10-7,10-6,10-5mol/L）后,检测精巢中Dmrtl mRNA的表达变化,探讨OP对Dmrt1基因表达的影响,分析Dmrt1可能的生理功能。采用反转录聚合酶链式反应（RT-PCR）和cDNA拼接技术,克隆中国林蛙Dmrt1基因,对其所编码的氨基酸序列信息,进化关系,蛋白质理化性质,二三级结构,亲水/疏水性,跨膜结构,信号肽,细胞定位进行分析。通过RT-PCR分析Dmrt1在成体精巢、卵巢、肝脏、肾脏和脑组织中的分布；对幼体性腺中肾复合体（GMC）中Dmrt1的表达变化也用RT-PCR进行分析；雌雄基因组中Dmrt1水平的差异用PCR技术进行简单评估。用荧光实时定量PCR检测不同OP浓度和不同暴露时间对Dmrt1mRNA表达的影响；用原位杂交技术检测Dmrt1m... 

【文章来源】：陕西师范大学陕西省 211工程院校 教育部直属院校

【文章页数】：71 页

【学位级别】：硕士

【文章目录】：
摘要
Abstract
Unit 1 Review on estrogen effects of 4-tert-octylphenol (OP)on reproductive and development in animal
    1. The distribution of OP and its effect of estrogen
    2. The effects of OP on animal reproductive and development
    3. The mechanism of endocrine activity interfered by OP
        3.1 OP paly roles in combination with estrogen receptor(ER)
        3.2 OP paly roles by interfering with the synthesis and metabolic pathway of steroid hormones
        3.3 Other possible ways
    4. The problems and prospect
Unit 2 Clonging and expression pattern of Double-sex and mab-3 relatedtranscription factor 1(Dmrt1)in frog Rana Chensinensis
    1. Introduction
    2. Materials and methods
        2.1 Materials
        2.2 Method
    3. Results and analysis
        3.1 The results of total RNA extraction
        3.2 Cloning and structure analysis of a full-length Dmrt1
        3.3 Dmrt1 expression in different tissues
        3.4 Expression analysis of Dmrt1 during R.chensinensis gonad differentiation and development
        3.5 Dmrt1 gene cloning from genomic DNA
    4. Discussion
    5. Conclusion
Unit 3 Effects of 4-tert-octylphenol on Dmrtl mRNAexpression in testes of frog Rana Chensmensis
    1. Introduction
    2. Materials and methods
        2.1 Materials
        2.2 OP exposure methods and sample collection
        2.3 In situ hybridization
        2.4 Real time fluorescent quantitative RT-PCR
        2.5 Photography and Statistical analysis
    3. Results
        3.1 The results of in situ hybridization of Dmrt1 mRNA
        3.2 Effects of OP on Dmrt1 mRNA expression
    4. Discussion
    5. Conclusion
Summarize
References
致谢
攻读硕士学位期间的研究成果


【参考文献】：
期刊论文
[1]两栖动物性别决定相关基因的研究进展[J]. 刘佳,李忻怡,张育辉.  动物学杂志. 2011(06)
[2]壬基酚对中国林蛙精巢中StAR表达的影响[J]. 李忻怡,王慧,张育辉.  环境科学研究. 2011(05)
[3]Distribution patterns of octylphenol and nonylphenol in the aquatic system at Mai Po Marshes Nature Reserve,a subtropical estuarine wetland in Hong Kong[J]. LI Xiang-li1,2,LUAN Tian-gang2,LIANG Yan1,,WONG Ming-hung1,LAN Chong-yu2,1.Croucher Institute for Environmental Sciences and Department of Biology,Hong Kong Baptist University,Hong Kong,China.2.State Key Laboratory of Biocontrol,School of life Sciences,Sun Yat-Sen(Zhongshan)University,Guangzhou 510275,China..  Journal of Environmental Sciences. 2007(06)



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