三种植物蛋白源分别替代鱼粉对凡纳滨对虾幼虾生长、血清生化指数和抗氧化功能的影响
发布时间：2022-01-11 18:59
本研究以大豆浓缩蛋白（Soybean Protein Concentrate,SPC）、小麦谷朊粉（Wheat Gluten Meal,WGM）和玉米酒精糟（Dried Distiller Grains with Solubles,DDGS）三种植物蛋白源分别替代鱼粉,进行养殖实验,分别考察饲料中SPC、WGM和DDGS替代FM对凡纳滨对虾（Litopaeneaus vannamei）生长、血清生化、血清抗氧化功能和抗病力的影响,以评估不同蛋白源替代鱼粉的适宜比例。实验一,选取凡纳滨对虾（初重为0.23±0.00 g）随机分为7组,每组3个重复,分别投喂大豆浓缩蛋白替代鱼粉蛋白水平为0%（1组）、3.08%（2组）、6.16%（3组）、9.24%（4组）、12.32%（5组）、15.40%（6组）和18.48%（7组）的饲料。结果显示:第6组的生长性能显著高于第1组（P<0.05）,且两组存活率均在90%-95%之间。各组的全虾体粗蛋白、水分和粗灰分含量无显著差异（P>0.05）。随着替代水平的升高,血清酸性磷酸酶（ACP）、碱性磷酸酶（AKP）、高密度脂蛋白胆固醇（HDL...
【文章来源】：广东海洋大学广东省
【文章页数】：124 页
【学位级别】：硕士
【文章目录】：
Abstract
摘要
LIST OF ABBREVIATIONS
1.GENERAL INTRODUCTION
1.1 Background study
1.1.1.Replacement of FM with SPC
1.1.2.Replacement of FM with WGM
1.1.3.Replacement of fishmeal with DDGS
1.2.Literature review
1.2.1.Animal species
1.2.2.Biology,morphology taxonomy and production of shrimp
1.2.3.Scientific classification
1.2.4.Distribution
1.2.5.Life stages
1.2.6.Protein requirement for L.vannamei
1.2.7.Amino acids in L.vannamei
1.3.Objectives
1.4.Significant of study
1.5.The null hypothesis to be tested
2.Effects of replacing fishmeal with dietary soybean protein concentrate(SPC)on growth,serum biochemical indices,and antioxidative functions for juvenile shrimp Litopenaeus vannamei
2.1.Introduction
2.2.Materials and methods
2.2.1.Experimental Diets
2.2.2.Diet preparation
2.2.3.Experimental conditions and sample collection
2.2.3.1.Experimental conditions
2.2.3.2.Sample collection
2.2.3.3.Growth performance index
2.2.4.Proximate composition analysis
2.2.5.Intestine and hepatopancreas sample collection
2.2.6.Serum biochemical analysis
2.2.7.Histological analysis
2.2.8.Intestinal flora analysis
2.2.9.Bacteria challenge test
2.2.10.Statistical analysis
2.3.Results
2.3.1.Effects of replacing FM with SPC on growth performance for juvenile Litopenaeus vannamei
2.3.2.Effects of replacing FM with SPC on proximate analysis of juvenile Litopenaeus vannamei
2.3.3.Effects of replacing FM with SPC on serum biochemical indices and antioxidant activities in juvenile Litopenaeus vannamei
2.3.4.Intestinal and hepatopancreas histological analysis
2.3.4.1.Intestinal histology analysis
2.3.4.2.Histological sections of the hepatopancreas
2.3.5.Intestinal flora analysis in shrimp gut
2.3.6.Bacteria challenge test results
2.4.Discussion
2.5.Conclusion
3.Effects of replacing fishmeal with dietary wheat gluten meal(WGM)on growth,serum biochemical indices,and antioxidative functions for juvenile shrimp Litopenaeus vannamei
3.1.Introduction
3.2.Materials and methods
3.2.1.Experimental diets and preparation
3.2.2.Experimental conditions and sampling
3.2.3.Sample collection and analysis
3.2.3.1.Growth performance
3.2.4.Feed and whole-body analysis
3.2.4.1.Blood biochemical measurements
3.2.4.2.Hepatic biochemical measurements
3.2.5.Histological assay of the midgut
3.2.6.Intestinal flora analysis
3.2.7.Challenge study
3.2.7.1.Preparation of inoculum bacteria
3.2.7.2.Calculation of fifty percent(50%)endpoint(Lethal Dose50)
3.2.7.3.Disease resistance test
3.2.8.Statistics
3.3.Results
3.3.1.Growth performance,survival,feed utilization
3.3.2.Proximate composition on diet
3.3.3.Effects of WGM on serum biochemical indices and antioxidant effects on shrimp
3.3.4.Histological analysis
3.3.5.Intestinal flora
3.3.6.Challenge test
3.4.Discussion
3.5.Conclusion
4.Effects of replacing fishmeal with dietary Dried Distiller Grains with Solubles(DDGS) on growth,serum biochemical indices,and antioxidative functions for juvenile shrimp Litopenaeus vannamei
4.1.Introduction
4.2.Materials and methods
4.2.1.Ingredients
4.2.1.2.Feed production using extrusion and pelleting
4.2.2.Fish husbandry and sampling
4.2.2.1.Growth measurements
4.2.3.Chemical analysis
4.2.4.Plasma analysis and antioxidant enzyme activities analysis
4.2.5.Intestinal morphometry
4.2.6.Gut microbiota
4.2.6.1.Total DNA extraction of the gut microbiome from shrimp
4.2.7.Challenge test
4.2.8.Statistical analysis
4.3.Results
4.3.1.Growth and survival performance
4.3.2.Proximate body composition in shrimp
4.3.3.Biochemical parameters of serum and antioxidant functions
4.3.3.1.Antioxidant activity and non-specific immunity
4.3.4.Histological analysis
4.3.5.Intestinal flora
4.3.6.Challenge test
4.4.Discussion
4.5.Conclusion
References
Acknowledgements
Author's profile
Supervisor's profile
【参考文献】：
期刊论文
[1]Antioxidant capacity and phytochemical content of 16 sources of corn distillers dried grains with solubles（DDGS）[J]. Eui-Cheol Shin,Gerald C.Shurson,Daniel D.Gallaher. Animal Nutrition. 2018(04)
[2]Effect of Replacing Fish Meal with Extruded Soybean Meal on Growth, Feed Utilization and Apparent Nutrient Digestibility of Juvenile White Shrimp（Litopenaeus vannamei）[J]. YANG Qihui,TAN Beiping,DONG Xiaohui,CHI Shuyan,LIU Hongyu. Journal of Ocean University of China. 2015(05)
本文编号：3583290
【文章来源】：广东海洋大学广东省
【文章页数】：124 页
【学位级别】：硕士
【文章目录】：
Abstract
摘要
LIST OF ABBREVIATIONS
1.GENERAL INTRODUCTION
1.1 Background study
1.1.1.Replacement of FM with SPC
1.1.2.Replacement of FM with WGM
1.1.3.Replacement of fishmeal with DDGS
1.2.Literature review
1.2.1.Animal species
1.2.2.Biology,morphology taxonomy and production of shrimp
1.2.3.Scientific classification
1.2.4.Distribution
1.2.5.Life stages
1.2.6.Protein requirement for L.vannamei
1.2.7.Amino acids in L.vannamei
1.3.Objectives
1.4.Significant of study
1.5.The null hypothesis to be tested
2.Effects of replacing fishmeal with dietary soybean protein concentrate(SPC)on growth,serum biochemical indices,and antioxidative functions for juvenile shrimp Litopenaeus vannamei
2.1.Introduction
2.2.Materials and methods
2.2.1.Experimental Diets
2.2.2.Diet preparation
2.2.3.Experimental conditions and sample collection
2.2.3.1.Experimental conditions
2.2.3.2.Sample collection
2.2.3.3.Growth performance index
2.2.4.Proximate composition analysis
2.2.5.Intestine and hepatopancreas sample collection
2.2.6.Serum biochemical analysis
2.2.7.Histological analysis
2.2.8.Intestinal flora analysis
2.2.9.Bacteria challenge test
2.2.10.Statistical analysis
2.3.Results
2.3.1.Effects of replacing FM with SPC on growth performance for juvenile Litopenaeus vannamei
2.3.2.Effects of replacing FM with SPC on proximate analysis of juvenile Litopenaeus vannamei
2.3.3.Effects of replacing FM with SPC on serum biochemical indices and antioxidant activities in juvenile Litopenaeus vannamei
2.3.4.Intestinal and hepatopancreas histological analysis
2.3.4.1.Intestinal histology analysis
2.3.4.2.Histological sections of the hepatopancreas
2.3.5.Intestinal flora analysis in shrimp gut
2.3.6.Bacteria challenge test results
2.4.Discussion
2.5.Conclusion
3.Effects of replacing fishmeal with dietary wheat gluten meal(WGM)on growth,serum biochemical indices,and antioxidative functions for juvenile shrimp Litopenaeus vannamei
3.1.Introduction
3.2.Materials and methods
3.2.1.Experimental diets and preparation
3.2.2.Experimental conditions and sampling
3.2.3.Sample collection and analysis
3.2.3.1.Growth performance
3.2.4.Feed and whole-body analysis
3.2.4.1.Blood biochemical measurements
3.2.4.2.Hepatic biochemical measurements
3.2.5.Histological assay of the midgut
3.2.6.Intestinal flora analysis
3.2.7.Challenge study
3.2.7.1.Preparation of inoculum bacteria
3.2.7.2.Calculation of fifty percent(50%)endpoint(Lethal Dose50)
3.2.7.3.Disease resistance test
3.2.8.Statistics
3.3.Results
3.3.1.Growth performance,survival,feed utilization
3.3.2.Proximate composition on diet
3.3.3.Effects of WGM on serum biochemical indices and antioxidant effects on shrimp
3.3.4.Histological analysis
3.3.5.Intestinal flora
3.3.6.Challenge test
3.4.Discussion
3.5.Conclusion
4.Effects of replacing fishmeal with dietary Dried Distiller Grains with Solubles(DDGS) on growth,serum biochemical indices,and antioxidative functions for juvenile shrimp Litopenaeus vannamei
4.1.Introduction
4.2.Materials and methods
4.2.1.Ingredients
4.2.1.2.Feed production using extrusion and pelleting
4.2.2.Fish husbandry and sampling
4.2.2.1.Growth measurements
4.2.3.Chemical analysis
4.2.4.Plasma analysis and antioxidant enzyme activities analysis
4.2.5.Intestinal morphometry
4.2.6.Gut microbiota
4.2.6.1.Total DNA extraction of the gut microbiome from shrimp
4.2.7.Challenge test
4.2.8.Statistical analysis
4.3.Results
4.3.1.Growth and survival performance
4.3.2.Proximate body composition in shrimp
4.3.3.Biochemical parameters of serum and antioxidant functions
4.3.3.1.Antioxidant activity and non-specific immunity
4.3.4.Histological analysis
4.3.5.Intestinal flora
4.3.6.Challenge test
4.4.Discussion
4.5.Conclusion
References
Acknowledgements
Author's profile
Supervisor's profile
【参考文献】：
期刊论文
[1]Antioxidant capacity and phytochemical content of 16 sources of corn distillers dried grains with solubles（DDGS）[J]. Eui-Cheol Shin,Gerald C.Shurson,Daniel D.Gallaher. Animal Nutrition. 2018(04)
[2]Effect of Replacing Fish Meal with Extruded Soybean Meal on Growth, Feed Utilization and Apparent Nutrient Digestibility of Juvenile White Shrimp（Litopenaeus vannamei）[J]. YANG Qihui,TAN Beiping,DONG Xiaohui,CHI Shuyan,LIU Hongyu. Journal of Ocean University of China. 2015(05)
本文编号：3583290
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