蓝莓花青素在人体细胞模型中调节糖脂代谢和血管舒张的作用机制
发布时间：2021-04-12 05:07
花青素是类黄酮化合物,具有多种生物活性,可预防和治疗多种与氧化应激相关的慢性疾病,包括心血管疾病、神经退行性疾病、癌症以及糖尿病。蓝莓果实中含有大量的花青素,在改善糖脂代谢、调节血压、预防糖尿病和高血压方面发挥重要作用。本项目研究了蓝莓花青素在HepG2细胞内对糖脂代谢的影响,并通过AMPK信号通路探讨蓝莓花青素对糖脂代谢的调控作用机理。此外,基于HUVEC细胞中eNOS-NO-PI3K-PKB/AKT-PPARγ通路,研究蓝莓花青素的抗氧化和血管舒张作用及其机制。本研究从细胞和分子水平上解析了蓝莓花青素的降血糖、降血脂和降血压作用,为开发和应用蓝莓花青素作为功能性食品成分或营养保健品提供理论依据。1、本研究采用超声波辅助提取法从南京种植的蓝莓果实中提取花青素。响应面法成功地用于优化蓝莓花青素的提取。最佳提取条件为:提取剂为0.02%盐酸溶液酸化的72.50%乙醇溶,液固比为20:1,提取时间为24 h,提取温度为30℃。此时花青素的产量达到16.21 ± 0.44 mg/g。采用AB-8大孔树脂纯化粗提物,最佳纯化条件为:洗脱液为0.02%盐酸溶液酸化的70.0%乙醇,吸附时间24h...
【文章来源】：南京师范大学江苏省 211工程院校
【文章页数】：114 页
【学位级别】：硕士
【文章目录】：
摘要
ABSTRACT
ABBREVATIONS AND ACRONYMS
CHAPTER Ⅰ. LITERATURE REVIEW
1. Blueberries
1.1. The characteristics of blueberries
1.2. Nutritional values of blueberries
1.3. Bioactive compounds of blueberries
1.4. Chemical structures of blueberry anthocyanins
1.5. Malvidin and its derivatives
2. Diabetes and glucolipid metabolism
2.1. Diabetes
2.2. Glucolipid metabolism
2.2.1. Glucose metabolism
2.2.2. Lipid metabolism
2.3. Hepatic oxidative stress and diabetes
3. Cardiovascular disease and vascular endothelium
3.1. Cardiovascular diseases
3.2. Vascular endothelium
3.3. Vascular oxidative stress and cardiovascular diseases
4. Functions of blueberry anthocyanins in human health
4.1. Anti-diabetes
4.2. Anti-hypertension
5. The aim of the study
5.1. Research objectives
5.2. The main research contents
CHAPTER Ⅱ. EXTRACTION, PURIFICATION, AND IDENTIFICATION OFANTHOCYANINS FROM BLUEBERRY FRUITS
1. Materials and equipments
1.1. Materials
1.2. Equipments
2. Methods
2.1. Single factor experiments in ultrasound-assisted extraction
2.2. Optimization using Box-Behnken response surface methodology
2.3. Determination of the total anthocyanin yield
2.4. Anthocyanin purification by AB-8 macroporous resin
2.5. Determination of adsorption capacity
2.6. Determination of the optimal concentration of eluent
2.7. Resin purification
2.8. Anthocyanin identification by HPLC
2.9. Anthocyanin identification by LC-ESI-MS
2.10. Statistical analysis
3. Results and discussion
3.1. Single factor experimental analysis of blueberry anthocyanin extraction
3.2. Optimization of blueberry anthocyanin extraction yield
3.3. Adsorption capacity, adsorption rate, and resolution rate
3.4. Optimum adsorption time
3.5. Optimum eluent concentration
3.6. Best purification conditions verification experiments
3.7. Identification of blueberry anthocyanins by HPLC and LC-ESI-MS
4. Conclusion
CHAPTER Ⅲ. ANTI-DIABETIC EFFECTS OF BLUEBERRY ANTHOCYANINEXTRACT ON GLUCOLIPID METABOLISM IN HIGH GLUCOSE-STIMULATED HEPG2 CELLS
1. Materials and equipments
1.1. Materials
1.2. Equipments
2. Methods
2.1. Cell culture and high glucose-stimulated diabetes treatments
2.2. MTT assay cell viability
2.3. ROS fluorescence visualization
2.4. ELISA
2.5. Western Blot
2.6. Statistical analysis
3. Results and discussion
3.1. Anti-diabetic effects of Mv,Mv-3-glc, Mv-3-gal, and BAE on cell viabilityin high glucose-stimulated HepG2 cells
3.2. Anti-oxidant effects of Mv, Mv-3-glc, Mv-3-gal, and BAE on ROS levelsin high glucose-stimulated HepG2 cells
3.3. Anti-diabetic effects of Mv,Mv-3-glc, Mv-3-gal, and BAE on glucose andlipid metabolism in high glucose-stimulated HepG2 supernatant and cells
3.3.1. The effects of Mv, Mv-3-glc, Mv-3-gal, and BAE on AMPKactivation
3.3.2. The effects of Mv, Mv-3-glc, Mv-3-gal, and BAE ongluconeogenesis
3.3.3. The effects of Mv, Mv-3-glc, Mv-3-gal, and BAE onglycogenolysis
3.3.4. The effects of Mv, Mv-3-glc, Mv-3-gal, and BAE on glucosetransporter 2
3.3.5. The effects of Mv, Mv-3-glc, Mv-3-gal, and BAE onlipogenesis
4. Conclusion
CHAPTER Ⅳ. ANTI-HYPERTENSIVE EFFECTS OF BLUEBERRYANTHOCYANIN EXTRACT ON ENDOTHELIAL VASODILATION IN HIGHGLUCOSE-STIMULATED HUVEC CELLS
1. Materials and equipments
1.1. Materials
1.2. Equipments
2. Methods
2.1. Cell culture and high glucose-stimulated hypertension treatments
2.2. MTT assay cell viability
2.3. ROS fluorescence visualization
2.4. ELISA
2.5. Western Blot
2.6. Statistical analysis
3. Results and discussion
3.1. Anti-hypertensive effects of Mv, Mv-3-glc, Mv-3-gal,and BAE on cellviability in high glucose-stimulated HUVEC cells
3.2. Anti-oxidant effects of Mv, Mv-3-glc, Mv-3-gal,and BAE on ROS levelsin high glucose-stimulated HUVEC cells
3.3. Anti-hypertensive effects of Mv, Mv-3-glc, Mv-3-gal, and BAE onendothelial vasodilatory in high glucose-stimulated HUVEC supernatant andcells
3.3.1. The effects of Mv, Mv-3-glc, Mv-3-gal, and BAE on the keyenzymes-induced vasodilation
3.3.2. The effects of Mv, Mv-3-glc, Mv-3-gal,and BAE on the keyenzymes-induced vasoconstriction
4. Conclusion
SUMMARY
INNOVATION AND FUTURE RESEARCH
REFERENCES
ACADEMIC PAPERS & PATENTS
ACKNOWLEDGEMENTS
本文编号：3132680
【文章来源】：南京师范大学江苏省 211工程院校
【文章页数】：114 页
【学位级别】：硕士
【文章目录】：
摘要
ABSTRACT
ABBREVATIONS AND ACRONYMS
CHAPTER Ⅰ. LITERATURE REVIEW
1. Blueberries
1.1. The characteristics of blueberries
1.2. Nutritional values of blueberries
1.3. Bioactive compounds of blueberries
1.4. Chemical structures of blueberry anthocyanins
1.5. Malvidin and its derivatives
2. Diabetes and glucolipid metabolism
2.1. Diabetes
2.2. Glucolipid metabolism
2.2.1. Glucose metabolism
2.2.2. Lipid metabolism
2.3. Hepatic oxidative stress and diabetes
3. Cardiovascular disease and vascular endothelium
3.1. Cardiovascular diseases
3.2. Vascular endothelium
3.3. Vascular oxidative stress and cardiovascular diseases
4. Functions of blueberry anthocyanins in human health
4.1. Anti-diabetes
4.2. Anti-hypertension
5. The aim of the study
5.1. Research objectives
5.2. The main research contents
CHAPTER Ⅱ. EXTRACTION, PURIFICATION, AND IDENTIFICATION OFANTHOCYANINS FROM BLUEBERRY FRUITS
1. Materials and equipments
1.1. Materials
1.2. Equipments
2. Methods
2.1. Single factor experiments in ultrasound-assisted extraction
2.2. Optimization using Box-Behnken response surface methodology
2.3. Determination of the total anthocyanin yield
2.4. Anthocyanin purification by AB-8 macroporous resin
2.5. Determination of adsorption capacity
2.6. Determination of the optimal concentration of eluent
2.7. Resin purification
2.8. Anthocyanin identification by HPLC
2.9. Anthocyanin identification by LC-ESI-MS
2.10. Statistical analysis
3. Results and discussion
3.1. Single factor experimental analysis of blueberry anthocyanin extraction
3.2. Optimization of blueberry anthocyanin extraction yield
3.3. Adsorption capacity, adsorption rate, and resolution rate
3.4. Optimum adsorption time
3.5. Optimum eluent concentration
3.6. Best purification conditions verification experiments
3.7. Identification of blueberry anthocyanins by HPLC and LC-ESI-MS
4. Conclusion
CHAPTER Ⅲ. ANTI-DIABETIC EFFECTS OF BLUEBERRY ANTHOCYANINEXTRACT ON GLUCOLIPID METABOLISM IN HIGH GLUCOSE-STIMULATED HEPG2 CELLS
1. Materials and equipments
1.1. Materials
1.2. Equipments
2. Methods
2.1. Cell culture and high glucose-stimulated diabetes treatments
2.2. MTT assay cell viability
2.3. ROS fluorescence visualization
2.4. ELISA
2.5. Western Blot
2.6. Statistical analysis
3. Results and discussion
3.1. Anti-diabetic effects of Mv,Mv-3-glc, Mv-3-gal, and BAE on cell viabilityin high glucose-stimulated HepG2 cells
3.2. Anti-oxidant effects of Mv, Mv-3-glc, Mv-3-gal, and BAE on ROS levelsin high glucose-stimulated HepG2 cells
3.3. Anti-diabetic effects of Mv,Mv-3-glc, Mv-3-gal, and BAE on glucose andlipid metabolism in high glucose-stimulated HepG2 supernatant and cells
3.3.1. The effects of Mv, Mv-3-glc, Mv-3-gal, and BAE on AMPKactivation
3.3.2. The effects of Mv, Mv-3-glc, Mv-3-gal, and BAE ongluconeogenesis
3.3.3. The effects of Mv, Mv-3-glc, Mv-3-gal, and BAE onglycogenolysis
3.3.4. The effects of Mv, Mv-3-glc, Mv-3-gal, and BAE on glucosetransporter 2
3.3.5. The effects of Mv, Mv-3-glc, Mv-3-gal, and BAE onlipogenesis
4. Conclusion
CHAPTER Ⅳ. ANTI-HYPERTENSIVE EFFECTS OF BLUEBERRYANTHOCYANIN EXTRACT ON ENDOTHELIAL VASODILATION IN HIGHGLUCOSE-STIMULATED HUVEC CELLS
1. Materials and equipments
1.1. Materials
1.2. Equipments
2. Methods
2.1. Cell culture and high glucose-stimulated hypertension treatments
2.2. MTT assay cell viability
2.3. ROS fluorescence visualization
2.4. ELISA
2.5. Western Blot
2.6. Statistical analysis
3. Results and discussion
3.1. Anti-hypertensive effects of Mv, Mv-3-glc, Mv-3-gal,and BAE on cellviability in high glucose-stimulated HUVEC cells
3.2. Anti-oxidant effects of Mv, Mv-3-glc, Mv-3-gal,and BAE on ROS levelsin high glucose-stimulated HUVEC cells
3.3. Anti-hypertensive effects of Mv, Mv-3-glc, Mv-3-gal, and BAE onendothelial vasodilatory in high glucose-stimulated HUVEC supernatant andcells
3.3.1. The effects of Mv, Mv-3-glc, Mv-3-gal, and BAE on the keyenzymes-induced vasodilation
3.3.2. The effects of Mv, Mv-3-glc, Mv-3-gal,and BAE on the keyenzymes-induced vasoconstriction
4. Conclusion
SUMMARY
INNOVATION AND FUTURE RESEARCH
REFERENCES
ACADEMIC PAPERS & PATENTS
ACKNOWLEDGEMENTS
本文编号：3132680
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