地中海果蝇bHLH基因及昆虫ASC基因鉴定与系统发生分析

发布时间：2021-01-01 07:17

　　碱性螺旋-环-螺旋（bHLH）蛋白组成了一个存在于真核生物中的转录因子超家族,在微小如酵母、大到如人类的生物中都有发现。它们在调控真核生物的神经发育、性别决定、肌肉生成、细胞命运决定以及响应环境信号等发育过程中发挥重要作用。bHLH这一名称来自于高度保守的结构基序,即包含约60个氨基酸、由一个碱性（basic）区域和两个被可变长度环分开的螺旋区域（HLH）组成的结构域。目前,根据bHLH蛋白与DNA结合的特性将其分为6个大组;此外,根据它们在调控不同发育与生理过程中的作用将它们分为45个家族。地中海果蝇（Ceratitis capitata）是广泛分布于世界各地的一种果蝇。它们以水果为食并在其中繁殖,因而对水果造成很大的物理及经济损害。为了控制地中海果蝇的危害,了解其生长发育机理具有重要意义。地中海果蝇的基因组测序计划在2016年9月即已完成,而其基因组中编码了多少个bHLH基因还未见报道。因此,我们开展了Blast搜索与系统发生分析来建立地中海果蝇bHLH（CcbHLH）基因的清单。无刚毛-盾片复合体（achaete-scute complex,ASC）基因编码在神经发育中发挥重要作... 

【文章来源】：江苏大学江苏省

【文章页数】：67 页

【学位级别】：硕士

【文章目录】：
ACKNOWLEDGEMENT
ABSTRACT
摘要
List of Abbreviations
Chapter1 Introduction
    1.1 bHLH transcription factors
        1.1.1 Animal bHLH transcription factors
        1.1.2 Insect bHLH family members
    1.2 Achaete-scute complex genes
        1.2.1 Functions of achaete-scute complex genes
        1.2.2 Classification of achaete-scute complex genes
    1.3 The Mediterranean fruit fly
        1.3.1 Morphology and habitat
        1.3.2 Reproduction and development
    1.4 Insects and food production
        1.4.1 Pests control in agriculture
        1.4.2 Insects as food
    1.5 Objectives and significance of this study
        1.5.1 Objectives
        1.5.2 Significance
    1.6 Research programs and technological schemes
        1.6.1 Research programs
        1.6.2 Technological schemes
Chapter2 Identification and phylogenetic analyses of Mediterranean fruit fly bHLH genes
    2.1 Materials and methods
        2.1.1 The45 representative bHLH motifs
        2.1.2 Blastp searches
        2.1.3 Multiple sequence alignment
        2.1.4 Phylogenetic analysis
        2.1.5 tBlastn searches
        2.1.6 Intron location
    2.2 Results and discussion
        2.2.1 Identification of CcbHLH family members
        2.2.2 Identification of orthologous families
        2.2.3 Identification of CcbHLH protein sequences
        2.2.4 Genomic coding regions of CcBHLH motifs
    2.3 Summary
Chapter3 Identification and phylogenetic analyses of insect achaete-scute complex genes
    3.1 Materials and methods
        3.1.1 Insect and other animal species
        3.1.2 Search and identification of ASC family members
        3.1.3 Phylogenetic analysis
        3.1.4 Display of conservative amino acids
        3.1.5 Location of ASC family genes
    3.2 Results and discussion
        3.2.1 New ASC gene family
        3.2.2 bHLH gene duplication in ASCa family
        3.2.3 Evolution of ASC genes in insects
    3.3 Summary
Chapter4 Conclusions and perspectives
    4.1 Conclusions
    4.2 Perspectives
References
Publications at Jiangsu University
Appendix



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