通过miRNA基因表达谱的基因共表达网络构建对星形细胞瘤的基因靶标进行预测
发布时间：2021-12-30 15:15
星形细胞瘤为浸润性生长肿瘤,生长缓慢,多为隐形症状,难以早期发现。多数肿瘤切除后有复发可能,且复发后肿瘤可演变成间变性星形细胞瘤或多形性胶质母细胞瘤。因此寻找其生物标志物早期诊断,并研究新的治疗方法就十分重要。方法:通过选用GEO数据库的星形细胞瘤miRNA表达谱数据,进行差异分析以及靶基因预测,通过2者共表达网络的构建对筛选出的miRNA的研究价值进行探讨。结果:得出hsa-miR-29b-2-5p;hsa-miR-339-5p与hsa-miR-362-3p3个较为关键的miRNA及与3者关系密切的8个mRNA。结论:通过对8个mRNA在癌症中的作用的讨论,肯定了这3个关键miRNA作为潜在靶点的研究价值。
【文章来源】：生物信息学. 2020,18(04)
【文章页数】：7 页
【部分图文】：
GSE138764数据集中筛选后的各5个上调和下调的差异miRNA热图
表 2 通过miRBD与Starbase v2.0预测与验证的差异miRNA的靶基因Table 2 Target genes of differential miRNAs predicted and verified by miRBD and Starbase v2.0 miRNAID Tatget gene miRNAID Target gene hsa-miR-29b-2-5p POU2F2 hsa-miR-505-3p HMGB1 hsa-miR-29b-2-5p WNK1 hsa-miR-505-3p DHX15 hsa-miR-29b-2-5p LRRTM2 hsa-miR-505-3p VGLL3 hsa-miR-29b-2-5p TTC17 hsa-miR-505-3p GLYR1 hsa-miR-29b-2-5p ZFHX3 hsa-miR-505-3p RBMS3 hsa-miR-29b-2-5p GUCY1A2 hsa-miR-505-3p SCLT1 hsa-miR-29b-2-5p EIF5B hsa-miR-505-3p LRRCC1 hsa-miR-29b-2-5p SGIP1 hsa-miR-505-3p SNX30 hsa-miR-29b-2-5p FAM160B1 hsa-miR-505-3p CREBRF hsa-miR-29b-2-5p MAK16 hsa-miR-505-3p PTBP2 hsa-miR-377-5p TMEM30B hsa-miR-339-5p MAPRE1 hsa-miR-377-5p AGO1 hsa-miR-339-5p BACE1 hsa-miR-377-5p ZFP82 hsa-miR-339-5p FAM219B hsa-miR-377-5p TFPI hsa-miR-339-5p WDR81 hsa-miR-377-5p DDA1 hsa-miR-339-5p NXPH1 hsa-miR-377-5p EIF5A2 hsa-miR-339-5p SLC15A2 hsa-miR-377-5p BATF2 hsa-miR-339-5p KMT2A hsa-miR-377-5p PRRC1 hsa-miR-339-5p SORT1 hsa-miR-377-5p MSL2 hsa-miR-339-5p DIPK2A hsa-miR-377-5p TRIO hsa-miR-339-5p C2orf83 hsa-miR-431-3p WDR48 hsa-miR-424-5p PAPPA hsa-miR-431-3p P2RY13 hsa-miR-424-5p FASN hsa-miR-431-3p ITGA11 hsa-miR-424-5p UNC80 hsa-miR-431-3p JAZF1 hsa-miR-424-5p FGF2 hsa-miR-431-3p C15orf62 hsa-miR-424-5p TNRC6B hsa-miR-431-3p PDE7A hsa-miR-424-5p PTPN4 hsa-miR-431-3p COPS9 hsa-miR-424-5p PHF19 hsa-miR-431-3p RBPMS hsa-miR-424-5p UBE2Q1 hsa-miR-431-3p DRAXIN hsa-miR-424-5p LSM11 hsa-miR-431-3p MTRNR2L3 hsa-miR-424-5p ANKUB1 hsa-miR-770-5p GMFB hsa-miR-362-3p PPP2R2D hsa-miR-770-5p MAP3K1 hsa-miR-362-3p PTBP2 hsa-miR-770-5p SLC17A6 hsa-miR-362-3p BLCAP hsa-miR-770-5p CCND2 hsa-miR-362-3p TNRC6B hsa-miR-770-5p MARK1 hsa-miR-362-3p TENT4B hsa-miR-770-5p DSE hsa-miR-362-3p DLGAP4 hsa-miR-770-5p ARHGAP12 hsa-miR-362-3p SMNDC1 hsa-miR-770-5p UBR3 hsa-miR-362-3p SRSF4 hsa-miR-770-5p PPP3CA hsa-miR-362-3p SHB hsa-miR-770-5p TDP1 hsa-miR-362-3p KLHL2 hsa-miR-485-5p KCNB1 hsa-miR-181a-2-3p FLI1 hsa-miR-485-5p PLXNA4 hsa-miR-181a-2-3p SORBS1 hsa-miR-485-5p PHTF2 hsa-miR-181a-2-3p BLOC1S5 hsa-miR-485-5p GPN3 hsa-miR-181a-2-3p PELI2 hsa-miR-485-5p SLC36A3 hsa-miR-181a-2-3p ARID4A hsa-miR-485-5p MSI2 hsa-miR-181a-2-3p DAZL hsa-miR-485-5p OGT hsa-miR-181a-2-3p ACVR2B hsa-miR-485-5p ADAMTSL5 hsa-miR-181a-2-3p SOX6 hsa-miR-485-5p ZBTB39 hsa-miR-181a-2-3p NETO2 hsa-miR-485-5p CKS1B hsa-miR-181a-2-3p RNF135与hsa-miR-29b-2-5p关系密切的关键mRNA为POU2F2与CCND2。在转移性GC细胞系和患者样品中检测到POU2F2表达增加。 POU2F2是由核因子(NF)-κB的激活诱导的,进而调节ROBO1转录,因此在功能上有助于GC转移。研究表明,NF-κB和以POU2F2为中心的SLIT2 / ROBO1相互作用网络可能对肿瘤转移起关键作用,miR-218为已知的能够调控POU2F2的miRNA,前体阻断面向POU2F2的转移网络的激活[11]。细胞周期蛋白D2(CCND2)是D型细胞周期蛋白的成员,在细胞周期调节,分化和恶性转化中起着关键作用。有研究表明明CCND2表达的降低与启动子异常甲基化密切相关[12]。
本文编号：3558461
【文章来源】：生物信息学. 2020,18(04)
【文章页数】：7 页
【部分图文】：
GSE138764数据集中筛选后的各5个上调和下调的差异miRNA热图
表 2 通过miRBD与Starbase v2.0预测与验证的差异miRNA的靶基因Table 2 Target genes of differential miRNAs predicted and verified by miRBD and Starbase v2.0 miRNAID Tatget gene miRNAID Target gene hsa-miR-29b-2-5p POU2F2 hsa-miR-505-3p HMGB1 hsa-miR-29b-2-5p WNK1 hsa-miR-505-3p DHX15 hsa-miR-29b-2-5p LRRTM2 hsa-miR-505-3p VGLL3 hsa-miR-29b-2-5p TTC17 hsa-miR-505-3p GLYR1 hsa-miR-29b-2-5p ZFHX3 hsa-miR-505-3p RBMS3 hsa-miR-29b-2-5p GUCY1A2 hsa-miR-505-3p SCLT1 hsa-miR-29b-2-5p EIF5B hsa-miR-505-3p LRRCC1 hsa-miR-29b-2-5p SGIP1 hsa-miR-505-3p SNX30 hsa-miR-29b-2-5p FAM160B1 hsa-miR-505-3p CREBRF hsa-miR-29b-2-5p MAK16 hsa-miR-505-3p PTBP2 hsa-miR-377-5p TMEM30B hsa-miR-339-5p MAPRE1 hsa-miR-377-5p AGO1 hsa-miR-339-5p BACE1 hsa-miR-377-5p ZFP82 hsa-miR-339-5p FAM219B hsa-miR-377-5p TFPI hsa-miR-339-5p WDR81 hsa-miR-377-5p DDA1 hsa-miR-339-5p NXPH1 hsa-miR-377-5p EIF5A2 hsa-miR-339-5p SLC15A2 hsa-miR-377-5p BATF2 hsa-miR-339-5p KMT2A hsa-miR-377-5p PRRC1 hsa-miR-339-5p SORT1 hsa-miR-377-5p MSL2 hsa-miR-339-5p DIPK2A hsa-miR-377-5p TRIO hsa-miR-339-5p C2orf83 hsa-miR-431-3p WDR48 hsa-miR-424-5p PAPPA hsa-miR-431-3p P2RY13 hsa-miR-424-5p FASN hsa-miR-431-3p ITGA11 hsa-miR-424-5p UNC80 hsa-miR-431-3p JAZF1 hsa-miR-424-5p FGF2 hsa-miR-431-3p C15orf62 hsa-miR-424-5p TNRC6B hsa-miR-431-3p PDE7A hsa-miR-424-5p PTPN4 hsa-miR-431-3p COPS9 hsa-miR-424-5p PHF19 hsa-miR-431-3p RBPMS hsa-miR-424-5p UBE2Q1 hsa-miR-431-3p DRAXIN hsa-miR-424-5p LSM11 hsa-miR-431-3p MTRNR2L3 hsa-miR-424-5p ANKUB1 hsa-miR-770-5p GMFB hsa-miR-362-3p PPP2R2D hsa-miR-770-5p MAP3K1 hsa-miR-362-3p PTBP2 hsa-miR-770-5p SLC17A6 hsa-miR-362-3p BLCAP hsa-miR-770-5p CCND2 hsa-miR-362-3p TNRC6B hsa-miR-770-5p MARK1 hsa-miR-362-3p TENT4B hsa-miR-770-5p DSE hsa-miR-362-3p DLGAP4 hsa-miR-770-5p ARHGAP12 hsa-miR-362-3p SMNDC1 hsa-miR-770-5p UBR3 hsa-miR-362-3p SRSF4 hsa-miR-770-5p PPP3CA hsa-miR-362-3p SHB hsa-miR-770-5p TDP1 hsa-miR-362-3p KLHL2 hsa-miR-485-5p KCNB1 hsa-miR-181a-2-3p FLI1 hsa-miR-485-5p PLXNA4 hsa-miR-181a-2-3p SORBS1 hsa-miR-485-5p PHTF2 hsa-miR-181a-2-3p BLOC1S5 hsa-miR-485-5p GPN3 hsa-miR-181a-2-3p PELI2 hsa-miR-485-5p SLC36A3 hsa-miR-181a-2-3p ARID4A hsa-miR-485-5p MSI2 hsa-miR-181a-2-3p DAZL hsa-miR-485-5p OGT hsa-miR-181a-2-3p ACVR2B hsa-miR-485-5p ADAMTSL5 hsa-miR-181a-2-3p SOX6 hsa-miR-485-5p ZBTB39 hsa-miR-181a-2-3p NETO2 hsa-miR-485-5p CKS1B hsa-miR-181a-2-3p RNF135与hsa-miR-29b-2-5p关系密切的关键mRNA为POU2F2与CCND2。在转移性GC细胞系和患者样品中检测到POU2F2表达增加。 POU2F2是由核因子(NF)-κB的激活诱导的,进而调节ROBO1转录,因此在功能上有助于GC转移。研究表明,NF-κB和以POU2F2为中心的SLIT2 / ROBO1相互作用网络可能对肿瘤转移起关键作用,miR-218为已知的能够调控POU2F2的miRNA,前体阻断面向POU2F2的转移网络的激活[11]。细胞周期蛋白D2(CCND2)是D型细胞周期蛋白的成员,在细胞周期调节,分化和恶性转化中起着关键作用。有研究表明明CCND2表达的降低与启动子异常甲基化密切相关[12]。
本文编号：3558461
本文链接：https://www.wllwen.com/projectlw/swxlw/3558461.html
教材专著
