大鼠心脏钝力性损伤的法医学实验研究

发布时间：2018-03-03 11:21

本文选题：钝性心脏损伤　切入点：基质金属蛋白酶　出处：《山西医科大学》2004年硕士论文　论文类型：学位论文

【摘要】：目的建立大鼠心脏钝力性损伤模型；观察损伤后正常区,损伤区心肌的病理变化；MMP-1和MMP-2的表达强度出现的规律；试图为心脏钝力性损伤的法医学鉴定提供客观的形态学依据，并探讨其可能的发生机制。方法健康成年Wistar大鼠50只随机分为七组即0h,6h,12h，24h,3天，5天，7天，0h为对照组：开胸，暴露心脏，逐层关胸，，待各组成活至相应时间时处死，取材。其余各组为：开胸，暴露心脏，打击，逐层关胸，到实验所需时间取心脏制作标本。HE染色，光镜观察；MMP-1及MMP-2免疫组织化学染色，计算机图象分析，统计学分析。结果（1）随时间的延长，MMP-1和MMP-2的表达强度呈现规律变化：MMP-1在伤后12h开始明显表达，逐渐增强，3d达高峰；MMP-2在伤后24h开始明显表达，五天达高峰。（2）HE染色可见心肌组织淤血，出血，炎细胞浸润，时间长的可有纤维化。（3）各损伤组图象分析结果与对照组结果经统计分析p＜0.05。结论（1）心脏损伤模型建立较成功；（2）对照组与实验组HE染色结果与免疫组织化学结果有较好的一致性，各指标的分布规律说明MMP-1及MMP-2可作为检测心肌损伤的指标加以研究。
[Abstract]:Objective to establish a rat model of cardiac blunt force injury, observe the pathological changes of myocardium in normal area and injured area after injury, and provide objective morphological basis for forensic identification of cardiac blunt force injury. The possible mechanism of its occurrence was also discussed. Methods Fifty healthy adult Wistar rats were randomly divided into seven groups: 0 h, 6 h, 12 h, 24 h, 3 days, 5 days, 7 days and 0 h as the control group: open chest, expose heart, close chest layer by layer, then kill each group after surviving to the corresponding time, the other groups were open chest and exposed heart. Beating, closing the chest layer by layer, taking the time to make heart specimen. HE staining, light microscope observation of MMP-1 and MMP-2 immunohistochemical staining, computer image analysis, statistical analysis. Results (1) the expression intensity of MMP-1 and MMP-2 showed a regular change with the extension of time, and the expression of MMP-1 began to appear at 12 hours after injury, gradually enhanced the expression of MMP-2 at the peak of 3 days after injury, and reached the peak at 24 hours after injury, and on the fifth day, congestion and bleeding of myocardial tissue were observed by HE staining. The results of image analysis of each injury group were compared with those of the control group (p < 0.05). Conclusion 1) the results of HE staining in the control group and the experimental group are in good agreement with the immunohistochemical results. The distribution of each index indicates that MMP-1 and MMP-2 can be used as indicators for the detection of myocardial injury.
【学位授予单位】：山西医科大学
【学位级别】：硕士
【学位授予年份】：2004
【分类号】：D919

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