大鼠脑外伤后神经细胞凋亡及脑红蛋白表达的实验研究

发布时间：2018-03-10 01:18

本文选题：外伤性脑损伤　切入点：脑红蛋白　出处：《四川大学》2006年硕士论文　论文类型：学位论文

【摘要】：研究背景及目的：脑红蛋白(Neuroglobin，Ngb)是一种主要位于脑内神经元的携氧球白，能可逆地结合O_2。在脑缺血缺氧时，脑红蛋白在神经元中表达增加，作为一种内源性神经保护因子可保护神经元免受缺血性损害。目前，对脑组织Ngb mRNA表达的研究主要通过采用颈动脉阻断的方法来建立缺血缺氧性脑损伤模型，而用外伤造成局灶性脑损伤模型来研究Ngb表达变化规律的方法，国内外文献尚未见报道。本研究采用硬膜外局部打击的方法建立大鼠外伤性局灶性脑损伤模型，研究伤后不同时间段和不同脑损伤区细胞凋亡和Ngb在mRNA水平表达变化规律，探讨脑外伤后Ngb对神经细胞的保护作用及其机制，以期为法医学损伤时间推断和颅脑损伤鉴定提供一种新的指标。方法：健康雄性SD大鼠随机分为正常对照组和脑损伤组。脑损伤组大鼠予木棒以70°的角度在硬膜外局部打击的方法造成SD大鼠外伤性局灶性脑挫伤模型。 (1)检测打击后脑挫伤区脑血流变化情况。 (2)正常对照组直接断颈处死大鼠，脑损伤组于伤后的不同时间段断颈处死大鼠，，取脑组织应用HE染色、TUNEL技术、半定量反转录—聚合酶链式反应(半定量RT-PCR)方法和图像分析技术对脑外伤后神经细胞凋亡、Ngb的表达变化规律进行研究。结果：(1)打击后脑损伤区血流量变化为：先短时升高，然后下降，并维持在低于正常的水平。(2)脑损伤组大鼠的脑损伤区Ngb表达在伤后15min开
[Abstract]:Background & AIM: Neuroglobinngb) is a oxygen-carrying globular white mainly located in the neurons of the brain, which can reversibly bind to O _ 2.The expression of neuroglobin in neurons increases during cerebral ischemia and hypoxia. As an endogenous neuroprotective factor, it can protect neurons from ischemic injury. At present, the expression of Ngb mRNA in brain tissue is mainly studied by carotid artery occlusion to establish the model of ischemic hypoxic brain injury. However, the method of studying the changes of Ngb expression in the model of traumatic focal brain injury has not been reported in domestic and foreign literature. In this study, the model of traumatic focal brain injury was established by using the method of epidural local strike in rats. To study the changes of apoptosis and expression of Ngb at mRNA level at different time points and different brain injury areas after injury, and to explore the protective effect of Ngb on nerve cells and its mechanism after brain injury. In order to provide a new index for forensic injury time estimation and craniocerebral injury identification. Methods: healthy male Sprague-Dawley rats were randomly divided into normal control group and brain injury group. 1) the changes of cerebral blood flow in the contusion area after striking were detected. (2) the rats in the normal control group were killed by direct cervical amputation, and the rats in the brain injury group were killed at different time points after injury. The brain tissue was taken out by HE staining and Tunel technique. Semi-quantitative reverse transcription-polymerase chain reaction (RT-PCR) and image analysis were used to study the expression of NGB in neuronal apoptosis after traumatic brain injury. Results the changes of blood flow in brain injury area after brain injury were as follows: (1) the expression of Ngb in brain injury area of rats in brain injury group was opened 15 minutes after injury, then decreased, and remained below the normal level.
【学位授予单位】：四川大学
【学位级别】：硕士
【学位授予年份】：2006
【分类号】：D919

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