一起死亡事故中难辨尸体的个体基因识别

发布时间：2018-04-11 09:56

本文选题：事故 + DNA指纹法　；参考：《解放军医学杂志》2006年10期

【摘要】：目的对某地一起死亡事故中难辨认尸体进行个体基因识别。方法从遇难者尸体取深部肌肉和软骨组织少许。对部分组织进行常规病理检查。从尸体组织提取基因组DNA,采用荧光多重PCR技术,扩增尸体基因组DNA的16个短串联重复序列(STR)基因座。将遇难者STR基因型与父母的STR基因型进行比对,确定遇难者的家庭归属。结果常规病理检查示部分肌肉组织结构完全破坏,但软骨组织结构尚完整。肌肉组织基因组DNA的凝胶电泳呈3种类型:基本无降解、部分降解、完全降解。只有部分肌肉组织DNA可用于确定STR基因型,所有软骨的DNA样本均可用于STR-PCR。结论以STR基因型分析为基础的亲子鉴定技术是确定事故遇难者身份的
[Abstract]:Objective to identify the individual gene of an unrecognizable corpse in a death accident.Methods the deep muscle and cartilage were taken from the bodies of the victims.Routine pathological examination was performed on some tissues.Genomic DNA was extracted from cadaveric tissues, and 16 short tandem repeats (STR) loci of genomic DNA were amplified by fluorescence multiple PCR technique.The STR genotypes of the victims were compared with the STR genotypes of their parents to determine the family ownership of the victims.Results routine pathological examination showed that partial muscle structure was completely destroyed, but cartilage tissue structure was intact.There were three types of gel electrophoresis of genomic DNA in muscle tissue: no degradation, partial degradation and complete degradation.Only part of muscle tissue DNA can be used to determine STR genotype, and all cartilage DNA samples can be used for STR-PCR.Conclusion the paternity test technique based on STR genotypic analysis is used to determine the identity of the victims of the accident.
【作者单位】：南京军区福州总医院分子医学研究中心南京军区福州总医院分子医学研究中心南京军区福州总医院分子医学研究中心南京军区福州总医院分子医学研究中心南京军区福州总医院分子医学研究中心南京军区福州总医院分子医学研究中心南京军区福州总医院分子医学研究中心南京军区福州总医院分子医学研究中心南京军区福州总医院分子医学研究中心南京军区福州总医院分子医学研究中心南京军区福州总医院分子医学研究中心
【分类号】：D919

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