线粒体DNA多态的变性高效液相色谱分析及其法医学应用研究

发布时间：2018-05-17 14:31

  本文选题：变性高效液相色谱(DHPLC) + 线粒体DNA(mtDNA)　； 参考：《四川大学》2004年博士论文

【摘要】：目的 建立新的mtDNA多态遗传标记的变性高效液相色谱检测技术,为法医学疑难检材的检测提供新的技术手段;利用变性高效液相色谱技术,寻找适合作为法医遗传标记的mtDNA编码区多态遗传标记,以期提高mtDNA在法医学检测中的应用价值。方法 应用primer3引物设计软件,对mtDNA控制区和编码区分别设计引物,用以进行PCR扩增。通过构建DNA池及样本间两两比较的方法,确定DHPLC发现异源双链的效能,从而建立mtDNA多态分析的DHPLC方法。用建立的方法,在成都汉族群体120名无关个体中,对mtDNA编码区各基因座进行多态性研究,获得等位基因及单倍型频率、变异度及标准误,并进行种属特异性、组织同一性以及陈旧骨骼、毛干等法医学疑难检材的DHPLC分析。结果 应用DHPLC技术,对两个mtDNA控制区基因座的扩增产物成功进行了异源双链分析,建立了适合法医学快速检测的DHPLC方法。针对mtDNA编码区总计1435bp范围,成功建立了DHPLC分析方法。根据我们设计的分析片段的位置,把此mtDNA编码区定义为7个基因座,分别称为C、D、E、F、G、H和I基因座。对七个mtDNA编码区基因座的多态性分析显示,变
[Abstract]:Objective to establish a new denaturing high performance liquid chromatography (DHPLC) technique based on mtDNA polymorphic genetic markers to provide a new method for the detection of difficult forensic materials, and to use denatured high performance liquid chromatography (DHPLC). In order to improve the application value of mtDNA in forensic medicine detection, the polymorphic genetic markers of mtDNA coding region were found to be suitable for forensic genetic markers. Methods primer3 primer design software was used to design primers for mtDNA control region and coding region for PCR amplification. The effectiveness of DHPLC in discovering heterologous double chains was determined by constructing a pairwise comparison between DNA pool and sample, and the DHPLC method for mtDNA polymorphism analysis was established. By using the established method, the allele and haplotype frequency, variation and standard error were obtained in 120 unrelated individuals in Chengdu Han nationality population. The allele and haplotype frequency, variation and species-specificity were obtained by studying the polymorphism of each locus in the mtDNA coding region. Tissue identity and DHPLC analysis of old bones, dried hair and other difficult forensic materials. Results the amplification products of two mtDNA control region loci were successfully analyzed by DHPLC, and a DHPLC method suitable for forensic rapid detection was established. Aiming at the total 1435bp range of mtDNA coding region, a DHPLC analysis method is established successfully. According to the position of the analyzed fragment, this mtDNA coding region is defined as seven loci, which are called Candruff FGG H and I loci, respectively. Polymorphic analysis of seven mtDNA loci showed that
【学位授予单位】：四川大学
【学位级别】：博士
【学位授予年份】：2004
【分类号】：D919

【参考文献】

相关期刊论文 前2条

1 侯一平,张思仲;温度调控高效液相色谱探索人类基因组变异的进展[J];中华医学遗传学杂志;2000年03期

2 廖林川,孟海英,侯一平,张思仲,颜有仪,苏智广,李英碧,吴瑾,张霁;用温度调控高效液相色谱探索基因组单核苷酸多态性的方法研究[J];中华医学遗传学杂志;2000年03期

，

本文编号：1901742