甲醛固定后豚鼠器官组织内头孢噻肟钠含量测定

发布时间：2018-05-25 02:11

本文选题：甲醛固定 + 头孢噻肟钠　；参考：《吉林大学》2007年硕士论文

【摘要】： 本研究利用法医毒化分析原理,采用科学的分离、提取方法和高科技检验手段,旨在通过动物实验证明,经甲醛固定后的豚鼠组织器官,一定时间段内可以检出头孢噻肟钠,以及观察一定时间段内头孢噻肟钠的定性、定量受甲醛固定处理的影响程度。实验通过对实验动物豚鼠给予适量静脉注射头孢噻肟钠,一定时间内处死后取材,固定,并在相应时间内进行甲醛固定后豚鼠组织器官中头孢噻肟钠的分离、提取,采用高效液相色谱法进行定性、定量。结果表明,经甲醛固定后的豚鼠组织器官,一定时间内可以检出头孢噻肟钠,头孢噻肟钠检出线性良好,方法快速、简便、灵敏、准确,回收率及精密度高。最终结论:经甲醛固定后豚鼠组织器官内可以检出头孢噻肟钠。固定后1个损失较多,固定后1个月至3个月内头孢噻肟钠检出量受甲醛固定的影响不大。
[Abstract]:The purpose of this study was to use the principle of forensic toxicological analysis, scientific separation, extraction method and high-tech test to prove that cefotaxime sodium could be detected in the tissues and organs of guinea pigs fixed by formaldehyde in a certain period of time. The qualitative and quantitative effects of formaldehyde fixation on cefotaxime sodium were observed. In the experiment, the guinea pigs were given appropriate amount of cefotaxime sodium intravenously, then killed and fixed in a certain time. The cefotaxime sodium was isolated and extracted from the tissues and organs of guinea pigs after formaldehyde fixation. High performance liquid chromatography (HPLC) was used for qualitative and quantitative analysis. The results showed that cefotaxime sodium and cefotaxime sodium could be detected in the tissues and organs of guinea pigs fixed by formaldehyde in a certain time. The method was rapid, simple, sensitive, accurate, high recovery and high precision. Conclusion: cefotaxime sodium can be detected in tissues and organs of guinea pigs after formaldehyde fixation. The amount of cefotaxime sodium was not affected by formaldehyde fixation within 1 to 3 months after fixation.
【学位授予单位】：吉林大学
【学位级别】：硕士
【学位授予年份】：2007
【分类号】：D919

【参考文献】