HIF-1α、IGF-1与脑损伤时间关系的实验研究

发布时间：2018-06-27 10:18

本文选题：低氧诱导因子-1α + 胰岛素样生长因子-1　；参考：《山西医科大学》2007年硕士论文

【摘要】： 目的:探讨大鼠脑损伤后低氧诱导因子-1α和胰岛素样生长因子-1表达的变化规律,旨为法医病理学中脑损伤时间的推断提供科学的参考依据。方法:健康成年Wistar大鼠72只,体重(250±10)g,雌雄不限,随机分为对照组、假手术组、伤后2h、6h、12h、24h、2d、3d、5d、7d、10d、14d组,每组6只。参照Feeneys法建立大鼠脑损伤模型,在伤后各时间点灌流固定处死大鼠,矢状面取材。通过HE染色和冰冻切片免疫组织化学法(SABC法)对各组HIF-1α、IGF-1蛋白的表达进行检测,用BI-2000图像分析系统测定阳性反应物平均灰度,用SPSS11.5统计软件进行统计学处理。结果:正常对照组神经细胞偶见HIF-1α表达,IGF-1表达呈弱阳性。脑损伤后HIF-1α和IGF-1的表达主要位于大脑皮质、脑干和海马的神经元细胞和神经胶质细胞中,并以神经胶质细胞为主,各部位变化趋势基本一致。伤后2h损伤区周围神经细胞可见HIF-1α蛋白阳性表达增加,6h后表达明显增强,24h达高峰,随后阳性反应细胞逐渐减少,7d后仍可见少量表达,14d后恢复(与对照组比较P=0.053);损伤后6h可见IGF-1蛋白表达增加,损伤后12h IGF-1表达显著增加,伤后3d达到峰值。此后逐渐回落,伤后10d表达仍高于对照组(P＜0.01),伤后14d基本恢复正常(与假手术组比较P=0.06)。损伤组与对照组及各相邻损伤组之间比较有统计学差异(P＜0.05)。结论:脑损伤后HIF-1α、IGF-1蛋白的表达具有一定的时间规律性,可望运用于法医学推断脑损伤时间。
[Abstract]:Objective: To investigate the changes in the expression of hypoxia inducible factor -1 alpha and insulin-like growth factor -1 after brain injury in rats, so as to provide a scientific reference for the inference of brain injury time in forensic pathology.
Methods: 72 healthy adult Wistar rats, body weight (250 + 10) g, and male and female, were randomly divided into control group, sham operation group, 2h, 6h, 12h, 24h, 2D, 3D, 5D, 7d, 10d, 6 in each group after injury. The expression of HIF-1 alpha and IGF-1 protein in each group was detected by histochemical method (SABC method). The average gray level of the positive reactant was measured by BI-2000 image analysis system, and the statistical software of SPSS11.5 was used for statistical processing.
Results: the expression of HIF-1 alpha was found in the normal control group, and the expression of IGF-1 was weakly positive. The expression of HIF-1 alpha and IGF-1 after brain injury was mainly located in the cerebral cortex, brain stem and hippocampus neurons and glial cells, mainly with neuroglia cells, and the changes in each part of the nerve cells were basically the same. The peripheral nerve cells in the 2H damaged area after injury were basically the same. The expression of HIF-1 alpha protein was increased, the expression of 24h was obviously enhanced, the expression of 24h reached the peak, and the positive reaction cells gradually decreased. After 7d, a small amount of expression was still visible. After 14d, the expression of IGF-1 protein was recovered (P=0.053). The expression of IGF-1 protein increased in 6h after injury and the expression of 12h IGF-1 increased significantly after injury, and the 3D reached the peak after injury, and then gradually fell, after the injury. The expression of 10d after injury was still higher than that in the control group (P < 0.01), and the 14d was basically recovered after injury (compared with the sham operation group P=0.06). There was a statistically significant difference between the injured group and the control group and the adjacent injury groups (P < 0.05).
Conclusion: the expression of HIF-1 alpha and IGF-1 protein after brain injury has certain time regularity, which is expected to be used to deduce the time of brain injury in forensic medicine.
【学位授予单位】：山西医科大学
【学位级别】：硕士
【学位授予年份】：2007
【分类号】：D919

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