温州汉族群体3个X-STR基因座遗传多态性
发布时间:2018-07-05 08:54
本文选题:法医物证学 + X染色体 ; 参考:《中国法医学杂志》2011年03期
【摘要】:正本文对402名温州汉族无关个体DXS9898、DXS6800、DXS6810等3个X-STR基因座遗传多态性多态性进行了调查,现报道如下。1材料与方法样本及DNA提取402名温州汉族无关个体抗凝血液样本,其中女220人、男182人,系本实验室日常检案积累。采用Chelex-100法[1]提取DNA。扩增及检测3个基因座的引物序列参照基因组数据库(www.gdb.org),由上海生工生物工程公司合成。PCR扩增条件为94℃3min;94℃35s,58℃
[Abstract]:The genetic polymorphisms of three X-STR loci were investigated in 402 unrelated individuals of Wenzhou Han nationality, including DXS9898, DXS6800 and DXS6810. The results are as follows: 1 samples of materials and methods and 402 samples of anticoagulant blood extracted by DNA from unrelated individuals of Wenzhou Han nationality, of which 220 were women. Male 182 people, is this laboratory daily inspection case accumulates. The method of Chelex-100 was used to extract DNA. The primer sequences for amplification and detection of three loci were amplified with reference to the genomic database (www.gdb.org). The conditions for the amplification of the primers from Shanghai Bioengineering Company were as follows: 94 鈩,
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