大鼠脑组织缺血后Ngb mRNA表达与缺血时间推断的研究
发布时间：2019-05-19 08:46
【摘要】： 1.大鼠脑组织缺血后Ngb mRNA表达与缺血时间推断的研究 目的:探讨大鼠脑组织缺血后不同时间脑红蛋白(Neuroglobin,Ngb)mRNA表达,旨为法医病理学实践中推断损伤时间提供科学的参考依据。 方法采用夹闭双侧颈总动脉法建立Wistar大鼠脑组织缺血模型,提取大鼠在脑缺血0min(对照组)、1min、5min、10min、30min、60min时脑组织中的总RNA,经质量评价后,无降解的RNA进行一步法逆转录-聚合酶链式反应(one step RT-PCR),以GAPDH为内参照,通过针对Ngb mRNA、GAPDH mRNA的特异性引物进行扩增,对扩增后的产物进行1.5%的琼脂糖凝胶电泳,然后利用凝胶图像分析技术检测Ngb mRNA和内参GAPDHmRNA的表达,并将二者的比值(Ngb mRNA/GAPDH mRNA)与缺血间隔时间进行相关性分析。 结果:(1)缺血后,Ngb mRNA在大鼠脑组织有明确而大量的表达。 (2)脑缺血后Ngb mRNA表达水平迅速升高,提示Ngb对缺氧反应非常敏感,可能对神经元起保护作用。 (3)Ngb mRNA的表达水平与缺血间隔时间有一定的相关性:缺血1min达高峰,与对照组比较差异有显著性(P＜0.01);缺血5min表达迅速降低,至10min仍维持在较低水平,与对照组相比差异无显著性(P＞0.05):随缺血时间延长,表达水平再度逐渐增强,与对照组相比差异有显著性(P＜0.01)。 结论:大鼠脑缺血缺氧后脑红蛋白的转录水平迅速增高,说明脑红蛋白对缺氧的反应非常敏感,可能参与了脑对缺氧的保护性反应。大鼠脑组织缺血后Ngb mRNA的表达随缺血时间的变化而有所不同,其与脑缺血间隔时间存在明显相关性,可在法医实践中用于早期脑缺血时间的推断。 2.人体腓骨性别判别分析 目的:探讨应用腓骨进行性别判别的方法,建立腓骨性别判别的函数判别式。 方法:采用腓骨5项测量指标,用人体测量仪器,按体质人类学测量方法,对国内9个不同省区、已知确切性别的305具尸骨(男274例,女31例,年龄范围18-84岁)的左、右侧共610根腓骨进行测量。将所测量数据作为变量输入计算机;使用社会科学统计软件包11.5(SPSS11.5),对左、右侧腓骨和男、女腓骨的统计学数据分别依据侧别和性别进行分组,然后进行两独立样本的t检验;筛选出具有显著性意义的测量指标,用Fisher判别分析法,建立腓骨性别判定的函数判别式。 结果:(1)男性或女性腓骨的左、右侧指标测量值的侧别差异均无统计学意义(P＞0.05),可以选择单侧进行研究。 (2)腓骨5项指标测量值的性别差异均具有极显著意义(P＜0.01),可以利用其进行性别判别。 (3)获得腓骨5个单指标函数判别式及1个多指标函数判别式,前者综合判别率在61.64%～75.74%,后者综合判别率达82.62%。 结论:腓骨单指标和多指标函数判别式可用于国人的性别判定,多指标函数判别式更具应用价值。
[Abstract]:1. Study on the expression of Ngb mRNA and the inference of ischemic time after cerebral ischemia in rats objective: to investigate the expression of brain red protein (Neuroglobin,Ngb) mRNA at different time after cerebral ischemia in rats. The purpose of this study is to provide a scientific reference for inferring the time of injury in the practice of forensic pathology. Methods the cerebral ischemia model of Wistar rats was established by clamping bilateral common carotid artery method. The total RNA, in brain tissue of rats with cerebral ischemia 0min (control group) was extracted at 10 min, 30 min and 60 min after 1 min, 5 min, 30 min and 60 min, and the total RNA, in brain tissue was evaluated. One-step reverse transcription-polymerase chain reaction (one step RT-PCR) of non-degraded RNA was carried out, and GAPDH was used as internal reference to amplify Ngb mRNA,GAPDH mRNA by specific primers. The amplified products were subjected to 1.5% agarose gel electrophoresis, and then the expression of Ngb mRNA and internal reference GAPDHmRNA was detected by gel image analysis, and the correlation between the ratio of Ngb mRNA/GAPDH mRNA and ischemic interval was analyzed. Results: (1), Ngb mRNA was clearly and heavily expressed in rat brain tissue after ischemia. (2) the expression of Ngb mRNA increased rapidly after cerebral ischemia, suggesting that Ngb is very sensitive to hypoxia and may play a protective role in neurons. (3) there was a certain correlation between the expression level of) Ngb mRNA and the interval between ischemia and ischemia: the peak of ischemic 1min reached the peak, which was significantly different from that of the control group (P < 0.01). The expression of ischemic 5min decreased rapidly and remained at a low level until 10min, which was not significantly different from that of the control group (P > 0.05). With the prolongation of ischemia time, the expression level increased gradually again. There was significant difference between the two groups (P < 0.01). Conclusion: the transcription level of brain red protein increases rapidly after cerebral ischemia and hypoxia in rats, which indicates that brain red protein is very sensitive to hypoxia and may be involved in the protective response of brain to hypoxia. The expression of Ngb mRNA after cerebral ischemia in rats is different with the change of ischemic time, and it is significantly correlated with the interval of cerebral ischemia, which can be used to infer the time of early cerebral ischemia in forensic practice. 2. Objective: to explore the method of sex discrimination of fibula and to establish a functional discriminant of sex discrimination of fibula. Methods: the left of 305 corpses of known sex (274males and 31 females, age range 18 鈮，
本文编号：2480579
[Abstract]:1. Study on the expression of Ngb mRNA and the inference of ischemic time after cerebral ischemia in rats objective: to investigate the expression of brain red protein (Neuroglobin,Ngb) mRNA at different time after cerebral ischemia in rats. The purpose of this study is to provide a scientific reference for inferring the time of injury in the practice of forensic pathology. Methods the cerebral ischemia model of Wistar rats was established by clamping bilateral common carotid artery method. The total RNA, in brain tissue of rats with cerebral ischemia 0min (control group) was extracted at 10 min, 30 min and 60 min after 1 min, 5 min, 30 min and 60 min, and the total RNA, in brain tissue was evaluated. One-step reverse transcription-polymerase chain reaction (one step RT-PCR) of non-degraded RNA was carried out, and GAPDH was used as internal reference to amplify Ngb mRNA,GAPDH mRNA by specific primers. The amplified products were subjected to 1.5% agarose gel electrophoresis, and then the expression of Ngb mRNA and internal reference GAPDHmRNA was detected by gel image analysis, and the correlation between the ratio of Ngb mRNA/GAPDH mRNA and ischemic interval was analyzed. Results: (1), Ngb mRNA was clearly and heavily expressed in rat brain tissue after ischemia. (2) the expression of Ngb mRNA increased rapidly after cerebral ischemia, suggesting that Ngb is very sensitive to hypoxia and may play a protective role in neurons. (3) there was a certain correlation between the expression level of) Ngb mRNA and the interval between ischemia and ischemia: the peak of ischemic 1min reached the peak, which was significantly different from that of the control group (P < 0.01). The expression of ischemic 5min decreased rapidly and remained at a low level until 10min, which was not significantly different from that of the control group (P > 0.05). With the prolongation of ischemia time, the expression level increased gradually again. There was significant difference between the two groups (P < 0.01). Conclusion: the transcription level of brain red protein increases rapidly after cerebral ischemia and hypoxia in rats, which indicates that brain red protein is very sensitive to hypoxia and may be involved in the protective response of brain to hypoxia. The expression of Ngb mRNA after cerebral ischemia in rats is different with the change of ischemic time, and it is significantly correlated with the interval of cerebral ischemia, which can be used to infer the time of early cerebral ischemia in forensic practice. 2. Objective: to explore the method of sex discrimination of fibula and to establish a functional discriminant of sex discrimination of fibula. Methods: the left of 305 corpses of known sex (274males and 31 females, age range 18 鈮，
本文编号：2480579
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