三株乳酸菌对小鼠肝脏抗氧化能力及肠道粘膜免疫的影响

发布时间：2018-09-10 08:00

【摘要】：选用三株乳酸菌LX5、HH7、AS8作为实验菌株,通过对小鼠肝脏抗氧化细胞因子及肠道粘膜免疫因子含量的检测,评价菌株对小鼠抗氧化能力及免疫力的影响。实验结果如下:1.试验期间LX5、HH7组及C组(对照组)小鼠体重均有明显的增加,增长率分别为41.6%、38.6%、23.2%;AS8组的小鼠体重稍有增加,增长率为9%,其中LX5与HH7组小鼠体重增长的最为迅速。灌胃期间,LX5、HH7组小鼠肠道菌群的乳酸菌、双歧杆菌数目增长,肠球菌、肠杆菌及产气芽孢梭菌数量下降;AS8组小鼠肠道菌群的乳酸菌、肠球菌数量增加,双歧杆菌数目略有降低,肠杆菌和产气芽孢杆菌数目降低;C组小鼠肠道菌群的数量基本无变化。2.灌胃组小鼠的肝重、脾重、肝体比和脾体比等指标,均与C组无明显差别(P0.05)。3.菌株LX5显著提高了 GSH-PX的含量(P0.05),显著降低了 MDA的含量(P0.05);菌株HH7显著或极显著增加了 T-AOC、T-SOD、GSH-PX的含量(P0.05,P0.01),显著降低了 MDA 的含量(P0.05);菌株 AS8 对 T-AOC、T-SOD、CAT、MDA、GSH-PX均无显著影响(P0.05)。总体来看,受试乳酸菌抗氧化能力由高到低为 HH7LX5AS8。4.菌株LX5显著或极显著升高了 SIgA、抗炎因子IL-2的含量(P0.05,P0.01),对促炎因子 TNF-α、IL-6、IL-8、NF-κB、iNOS、JNK、AP-1 及对抗炎因子 IL-10无显著影响(P0.05);菌株HH7显著或极显著提高了 SIgA、抗炎因子IL-2、IL-10的含量(P0.05,P0.01),显著或极显著降低了促炎因子TNF-α、NF-κB、iNOS的含量(P0.05,P0.01),对促炎因子 IL-6、IL-8、JNK、AP-1 无显著影响(P0.05);菌株AS8显著或极显著提高了 SIgA、抗炎因子IL-2、IL-10和促炎因子NF-κB的含量(P0.05,P0.01),对促炎因子 TNF-α、IL-6、IL-8、iNOS、JNK、AP-1 无显著影响(P0.05)。三株试验菌株均对小鼠肠道免疫有一定的影响,且三者存在菌株特异性。菌株LX5、HH7均有抗炎作用,且菌株HH7的抗炎作用要优于菌株LX5。5.菌株HH7鉴定为干酪乳杆菌(Lactobacilluscasei);菌株LX5鉴定为副干酪乳杆菌(Lactobacillus paracasei);菌株 AS8 鉴定为屎肠球菌(Enterococcus faecium)。6.AS8组小鼠小肠肠道电镜扫描,电镜结果显示:肠道绒毛杂乱无章,撕裂,倒伏,呈枯萎状,肠内壁裸露、多孔,AS8组小鼠肠道受损,表明AS8菌株存在一定的安全性问题。
[Abstract]:Three strains of lactic acid bacteria (LX5,HH7,AS8) were used as experimental strains to evaluate the effects of Lactic acid bacteria (LX5,HH7,AS8) on the antioxidant capacity and immunity of mice by detecting the contents of antioxidant cytokines and intestinal mucosal immune factors in mice. The results of the experiment are as follows: 1. During the trial period, the body weight of LX5,HH7 group and C group (control group) increased obviously, the growth rate was 41.6% and 38.6% respectively, and the growth rate was 9% in AS8 group, especially in LX5 group and HH7 group. During intragastric administration, the number of Lactobacillus and Bifidobacterium in the intestinal flora of mice in the LX5 / HH7 group increased, while the number of Enterococcus, Enterobacter and Clostridium aerogenes decreased. The number of enterococci increased and the number of Bifidobacterium decreased slightly in the AS8 group. The number of Enterobacter and Bacillus aerogenes decreased the number of intestinal microflora in group C was basically unchanged. 2. 2. There was no significant difference in liver weight, spleen weight, liver-body ratio and splenic body ratio between group C and group C (P0.05). Strain LX5 significantly increased the content of GSH-PX (P0.05), significantly decreased the content of MDA (P0.05), significantly or extremely significantly increased the content of T-AOCT-SODX GSH-PX (P0.05P0.01), significantly decreased the content of MDA (P0.05), and strain AS8 had no significant effect on T-AOCT-SODCATDAMDA-GSH-PX (P0.05). In general, the antioxidant capacity of lactic acid bacteria tested was from high to low to HH7LX5AS8.4.. LX5 significantly or very significantly increased the content of SIgA, anti-inflammatory factor IL-2 (P0.05, P0.01), but had no significant effect on TNF- 伪 IL-6, IL-8, NF- 魏 BN-iNOS-1 and anti-inflammatory factor IL-10 (P0.05), while HH7 significantly or extremely increased the content of SIgA, anti-inflammatory factor IL-2,IL-10 (P0.05P0.01), significantly or very significantly (P0.01), and significantly or extremely significantly increased the content of SIgA, anti-inflammatory factor IL-2,IL-10 (P0.05, P0.01), and significantly or extremely significantly increased the content of SIgA, anti-inflammatory factor IL-2,IL-10 (P0.05P0.01). The content of TNF- 伪 -NF- 魏 B iNOS was significantly decreased (P0.05, P0.01), and the content of SIgA, anti-inflammatory factor IL-2,IL-10 and NF- 魏 B was significantly increased (P0.05, P0.01), while the content of SIgA, anti-inflammatory factor IL-2,IL-10 and pro-inflammatory factor NF- 魏 B was significantly increased (P0.05, P0.01), while the content of SIgA, anti-inflammatory factor IL-2,IL-10 and pro-inflammatory factor NF- 魏 B was significantly increased (P0.05, P0.01), but it had no significant effect on the pro-inflammatory factor TNF- 伪 IL-8IL-8iNOSNK-JNKAP-1 (P0.05), but the content of SIgA, anti-inflammatory factor IL-2,IL-10 and NF- 魏 B was significantly increased (P 0.05, P 0.01). All of the three strains had a certain effect on the intestinal immunity of mice, and the three strains were specific. All strains of LX5,HH7 have anti-inflammatory effect, and the anti-inflammatory effect of strain HH7 is better than that of strain LX5.5.. HH7 was identified as Lactobacillus casei (Lactobacilluscasei);, LX5 was identified as Lactobacillus casei (Lactobacillus paracasei);, AS8 was identified as Enterococcus faecium (Enterococcus faecium) .6.The intestinal tract of mice in AS8 group was scanned by electron microscope. The results of electron microscope showed that the intestinal villi were disorganized, torn and lying down. The intestinal tract was damaged in AS8 group, indicating that the AS8 strain had some safety problems.
【学位授予单位】：内蒙古农业大学
【学位级别】：硕士
【学位授予年份】：2017
【分类号】：TS201.3

【相似文献】