氢气对盐胁迫下喜盐鸢尾氧化损伤保护机制研究

发布时间：2019-03-26 15:41

【摘要】：全球土壤盐渍化日益严重,我国盐渍土面积已显著高于全球平均水平。盐胁迫严重影响植物生长发育,盐生植物与盐敏感植物相比在应答盐胁迫的过程中可能存在独特的调控机制,本研究利用耐盐植物喜盐鸾尾(Iris.halophila Pall.)作为研究对象来探讨其耐盐机理。氢气(Hydrogen,H2)作为一种新型调节植物生理过程中的信号分子,可通过影响植物激素、调节离子平衡的转运蛋白及其转录因子、抗氧化酶、根际微生物等参与和调节植物生长发育、成熟衰老、胁迫耐受等多种生理过程。本研究对H2是否可以增强喜盐鸢尾盐耐受性展开研究,以期为进一步提升耐盐植物耐受性提供新的思路。主要研究结果如下:1.喜盐鸢尾受到盐胁迫后通过提高抗氧化酶活性、可溶性蛋白含量及控制Na+转运相关基因以减缓盐处理对愈伤组织氧化损伤程度。2.用 50、100、500mmol/L NaCl 及 25%、50%、100%富氢水(Hydrogen-rich water,HRW)混合溶液处理喜盐鸢尾愈伤组织,结果显示25%、50%、100%HRW可不同程度缓解盐胁迫对喜盐鸢尾造成的损伤,其中100%HRW效果最佳。3.HRW处理可提高抗氧化酶(SOD、POD、可溶性蛋白)的活性,减缓盐处理对愈伤组织氧化损伤程度。4.HRW处理可显著提高喜盐鸢尾NHX基因的表达量,降低钠钾离子比,增强喜盐鸢尾耐盐性。5.HRW处理可提高喜盐鸢尾内源NO含量,而NO已被证明可参与到植物耐盐调控中。本研究采用生理生化、化学及分子手段对HRW的保护作用进行探究,研究结果表明HRW处理喜盐鸢尾可提高其抗氧化酶活性,增加NHX基因表达量,降低钠钾离子比,提高内源NO含量,说明HRW可能通过调节Na+转运及提高抗氧化活性等方式减轻盐胁迫损伤,这一结果为进一步探究氢气的胁迫保护作用提供了坚实基础。
[Abstract]:The salinization of the global soil is becoming more and more serious, and the area of saline soil in China has been significantly higher than that of the global average. Salt stress seriously affects the growth and development of the plant, and the salt-sensitive plant may have a unique control mechanism in response to salt stress in response to salt stress. ) The salt-tolerant mechanism of the study was discussed. hydrogen (HEMAgen, H2) is used as a signal molecule in a novel regulation plant physiological process, and can participate in and regulate the growth and development of a plant by influencing the plant hormone, regulating the transport protein of the ion balance and the transcription factor, the antioxidant enzyme, the rhizosphere microorganism, and the like, Stress tolerance and other physiological processes. In order to provide a new way to further improve the tolerance of salt-tolerant plants, the study was conducted to investigate whether H2 could enhance the tolerance of salt-tolerant plants. The main results are as follows:1. The effect of salt stress on the activity of antioxidant enzymes, the content of soluble protein and the control of Na + transport-related genes to reduce the degree of oxidative damage to the callus after salt stress. The results showed that 50%,100,500 mmol/ L NaCl and 25%,50%,100% hydrogen-rich water (HRW) were used to treat the callus of the iris. The results showed that 25%,50%,100% HRW could alleviate the damage to the iris of the iris by salt stress, and the effect of 100% HRW was the best.3. The treatment of HRW could improve the antioxidant enzymes (SOD, POD, 4. The treatment of HRW could increase the content of endogenous NO in the iris of the iris. NO has been shown to be involved in plant salt tolerance regulation. In this study, the effects of physiological, biochemical, chemical and molecular mechanisms on the protection of HRW were studied. The results showed that HRW could increase the activity of antioxidant enzymes, increase the expression of NHX, decrease the ratio of sodium and potassium ions, and improve the content of endogenous NO. It is suggested that HRW can reduce the salt stress damage by adjusting Na + transport and improving the anti-oxidation activity. The result provides a solid foundation for further exploring the stress protection of hydrogen.
【学位授予单位】：中央民族大学
【学位级别】：硕士
【学位授予年份】：2017
【分类号】：Q945.78

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