水稻顶端穗退化tsr突变体的基因定位及候选基因的克隆
发布时间：2019-07-04 15:48
【摘要】：水稻顶端穗退化往往会导致秃尖现象的发生,因而带来穗粒数减少,单株产量降低,低结实率,产量大幅下降。因此,引起了许多育种家的重视,但其遗传基础并不十分清晰。本实验利用SSR和INDEL分子标记技术结合BSA分析法来对顶端小穗退化突变体tsr基因定位和候选基因的克隆。主要研究结果如下：(1)tsr突变体穗顶部的颖花发育不完整,在成熟的后期这些发育不完整的颖花变干脱落,呈现形态上的秃穗。与原品种168比较,tsr突变体的株高较矮,稻穗变小、粒数减少、结实率降低,且存在包颈现象。(2)遗传分析表明,该顶端小穗退化突变体受一对隐性核基因控制。(3)将顶端小穗退化突变体与籼稻品种5364s杂交后自交的F2代作为定位群体。采用BSA和SSR分子标记相结合的方法将穗退化基因初步定位在第3号染色体短臂RM157A瀣处。(4)通过Indel引物的设计,最终将穗退化基因精细定位在两个紧密连锁的Indel分子标记hc42和hc58的67kb之间,该区间共有9个候选基因。(5)对候选基因Os03g0319300和Os03g0320000测序,结果发现Os03g0320000基因的第129氨基酸位点发生C→G的点突变,由丙氨酸变为甘氨酸。
[Abstract]:The degeneration of top panicle of rice often leads to the occurrence of baldness, which leads to the decrease of grain number per panicle, the decrease of yield per plant, the low seed setting rate and the decrease of yield. Therefore, many breeders pay attention to it, but its genetic basis is not very clear. In this experiment, SSR and INDEL molecular markers combined with BSA analysis were used to locate the apical spikelet mutant tsr gene and clone the candidate genes. The main results are as follows: (1) the spikelet at the top of tsr mutant is incomplete, and these incomplete spikelets become dry and fall off at the later stage of maturity, showing morphological baldness. Compared with the original variety 168, the plant height of tsr mutant was shorter, the panicle became smaller, the grain number decreased, and the seed setting rate decreased, and there was a phenomenon of neck inclusion. (2) genetic analysis showed that the apical spikelet degeneration mutant was controlled by a pair of recessive nuclear genes. (3) the F2 generation inbred with indica rice variety 5364s was used as the mapping population. The ear degeneration gene was initially located in the short arm RM157A of chromosome 3 by the combination of BSA and SSR molecular markers. (4) through the design of Indel primers, the ear degeneration gene was finely mapped between two closely linked Indel molecular markers hc42 and hc58 67kb, and there were 9 candidate genes in this region. (5) the candidate genes Os03g0319300 and Os03g0320000 were sequenced. The results showed that C 鈮，
本文编号：2510029
[Abstract]:The degeneration of top panicle of rice often leads to the occurrence of baldness, which leads to the decrease of grain number per panicle, the decrease of yield per plant, the low seed setting rate and the decrease of yield. Therefore, many breeders pay attention to it, but its genetic basis is not very clear. In this experiment, SSR and INDEL molecular markers combined with BSA analysis were used to locate the apical spikelet mutant tsr gene and clone the candidate genes. The main results are as follows: (1) the spikelet at the top of tsr mutant is incomplete, and these incomplete spikelets become dry and fall off at the later stage of maturity, showing morphological baldness. Compared with the original variety 168, the plant height of tsr mutant was shorter, the panicle became smaller, the grain number decreased, and the seed setting rate decreased, and there was a phenomenon of neck inclusion. (2) genetic analysis showed that the apical spikelet degeneration mutant was controlled by a pair of recessive nuclear genes. (3) the F2 generation inbred with indica rice variety 5364s was used as the mapping population. The ear degeneration gene was initially located in the short arm RM157A of chromosome 3 by the combination of BSA and SSR molecular markers. (4) through the design of Indel primers, the ear degeneration gene was finely mapped between two closely linked Indel molecular markers hc42 and hc58 67kb, and there were 9 candidate genes in this region. (5) the candidate genes Os03g0319300 and Os03g0320000 were sequenced. The results showed that C 鈮，
本文编号：2510029
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