有序胶原支架复合SDF-1治疗骨软骨缺损的作用及机制研究

发布时间：2018-01-16 17:01

本文关键词：有序胶原支架复合SDF-1治疗骨软骨缺损的作用及机制研究　出处：《浙江大学》2017年硕士论文　论文类型：学位论文

【摘要】：目的胶原是治疗骨软骨缺损的一种常用生物材料,然而目前胶原支架的结构大多是无序的,因此不利于自体细胞向缺损部位迁移进行缺损修复。基质细胞衍生因子-1(SDF-1)被认为能够促进间充质干细胞向软骨下骨迁移。本实验目的是通过设计有取向通道的胶原支架复合SDF-1来促进细胞向缺损部位迁移,从而促进骨软骨缺损的修复和治疗。方法我们首先通过胶原冻干和热交联制作了无序胶原支架和有序胶原支架,分别用电镜对支架通道进行表征,并对它们进行了力学性能,细胞毒性,细胞增殖等检测。然后我们检测了骨髓间充质干细胞在材料内的迁移。最后,我们在新西兰兔上建立了骨软骨缺损模型,然后将两种胶原支架分别复合SDF-1移植到缺损部位,分别在6周和12周收集样本,通过ICRS评分、番红-固绿染色以及免疫组化染色检测软骨再生情况。结果有序胶原支架较无序胶原支架有更好的力学强度,两种材料对细胞增殖无明显影响。有序胶原支架可以促进骨髓间充质干细胞的迁移,该作用能够被SDF-1加强。动物实验结果表明结合有序胶原支架复合细胞因子SDF-1可以促进骨软骨缺损部位新生软骨的生长,同时能够抑制软骨细胞的终末分化和钙化。结论通过有序胶原支架复合SDF-1的移植是一种可行的骨软骨缺损疗法。
[Abstract]:Objective Collagen is a commonly used biomaterial for the treatment of osteochondral defects, but the structure of collagen scaffold is disordered. This is not conducive to the migration of autologous cells to the defect site for repair. Stromal Cell derived Factor-1 SDF-1). It is believed that mesenchymal stem cells can promote the migration of mesenchymal stem cells to subchondral bone. In order to promote the repair and treatment of osteochondral defects. Methods We first prepared the disorder collagen scaffold and the ordered collagen scaffold through the collagen freeze-drying and thermal crosslinking, and used electron microscope to characterize the scaffold channel. Then we detected the migration of bone marrow mesenchymal stem cells in materials. Finally, we established a bone cartilage defect model in New Zealand rabbits. Then the two collagen scaffolds combined with SDF-1 were transplanted to the defect site, and the samples were collected at 6 and 12 weeks, respectively, and the ICRS score was used. Results the ordered collagen scaffold had better mechanical strength than the disordered collagen scaffold. The two materials have no significant effect on cell proliferation. Ordered collagen scaffolds can promote the migration of bone marrow mesenchymal stem cells. The effect could be enhanced by SDF-1. The results of animal experiments showed that the combination of ordered collagen scaffold and cytokine SDF-1 could promote the growth of new cartilage in bone cartilage defect. At the same time, it can inhibit the terminal differentiation and calcification of chondrocytes. Conclusion the transplantation of SDF-1 combined with ordered collagen scaffold is a feasible treatment for osteochondral defects.
【学位授予单位】：浙江大学
【学位级别】：硕士
【学位授予年份】：2017
【分类号】：R318.08

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