大肠湿热证管状腺瘤与P53、bFGF表达的相关性研究

发布时间：2018-01-31 19:28

本文关键词： 大肠管状腺瘤大肠湿热证 P53 bFGF　出处：《福建中医药大学》2017年硕士论文　论文类型：学位论文

【摘要】：目的:探讨大肠湿热证管状腺瘤与P53、bFGF表达的相关性。方法:随机选取就诊福建省第二人民医院门诊/病房行电子结肠镜检查发现息肉并行息肉摘除术,经病理诊断确诊为大肠管状腺瘤伴IN并符合纳入标准的患者60例,按中医辨证分为研究组(大肠湿热证组)、对照组(脾胃虚弱证组)各30例,并设立正常对照组20例(来源于本院体检中心)。通过免疫组织化学方法检测研究组、对照组及正常对照组肠黏膜组织中P53及bFGF的表达水平,统计上述3组P53、bFGF表达并进行对比分析,探讨它们之间的相关性。结果:1、对大肠湿热证组、脾胃虚弱证组和正常对照组的年龄、性别进行比较,P均0.05,无统计学意义,三组间的性别、年龄无显著差异,具有可比性。对研究组和对照组的瘤变程度进行比较,P0.05,两中医证型间腺瘤瘤变程度具有可比性。2、统计大肠管状腺瘤P53、bFGF的表达与性别、年龄、上皮内瘤变程度的关系,发现P53、bFGF的表达在性别、年龄上无差异(P0.05);在低高级别上皮内瘤变程度中的表达差异经检验有统计学意义(P0.05),且成正相关。3、P53、bFGF在大肠湿热证组、脾胃虚弱证组表达均明显高于正常对照组,差异有统计学意义(P0.05);大肠湿热证组中P53、bFGF表达与脾胃虚弱证组比较差异无统计学意义(P0.05)。4、大肠管状腺瘤同一高级别上皮内瘤变大肠湿热证P53表达高于脾胃虚弱证,具统计学意义(P0.05);不同瘤变程度大肠湿热证组、脾胃虚弱证组中bFGF表达均无差异(P0.05)。5、大肠湿热证组和脾胃虚弱证组中P53、bFGF在高级别上皮内瘤变中的表达均明显高于低级别上皮内瘤变,具有统计学意义(P均0.05)。结论:1、大肠管状腺瘤高级别上皮内瘤变中P53表达与大肠湿热证可能存在相关。2、P53、bFGF在大肠管状腺瘤组织中的表达明显高于正常肠黏膜组织,且高级别上皮内瘤变中表达明显升高,提示P53及bFGF在腺瘤恶变过程中可能起重要作用。
[Abstract]:Objective: to investigate the relationship between tubular adenoma and p53 in large intestine damp-heat syndrome. Correlation of bFGF expression. Methods: polyps were detected by electronic colonoscopy in outpatient / ward of the second people's Hospital of Fujian Province. Sixty patients with tubular adenoma of large intestine with IN and according to the inclusion criteria were divided into two groups according to TCM syndrome differentiation (30 cases in the group of damp-heat syndrome of large intestine and 30 cases in the group of deficiency of spleen and stomach). The expression levels of p53 and bFGF in intestinal mucosa of the study group, the control group and the normal control group were detected by immunohistochemical method. The expression of P53 bFGF in the above three groups was analyzed and compared, and the correlation between them was discussed. Results: 1. The age of the group with damp-heat syndrome of large intestine, the group of deficiency of spleen and stomach and the normal control group. Gender comparison P 0.05, no statistical significance, three groups of gender, age no significant difference, comparable. The study group and the control group of the degree of neoplasia were compared P0.05. The expression of P53 bFGF in colorectal tubular adenoma was correlated with sex, age and degree of intraepithelial neoplasia. The expression of bFGF was not different in sex and age (P 0.05). The difference of expression in the low and high grade intraepithelial neoplasia was statistically significant (P 0.05), and the positive correlation was found in the large intestine dampness and heat syndrome group. The expression of spleen and stomach deficiency syndrome group was significantly higher than that of normal control group, and the difference was statistically significant (P 0.05). The expression of P53 bFGF in the large intestine damp-heat syndrome group was not significantly different from that in the spleen and stomach asthenia group. The expression of p53 in large intestine damp-heat syndrome was higher than that in spleen and stomach asthenia group (P 0.05). There was no difference in the expression of bFGF in the groups with different degrees of tumorigenesis of dampness and heat of large intestine and deficiency of spleen and stomach, and p53 in the group of damp-heat of large intestine and the group of deficiency of spleen and stomach. The expression of bFGF in high grade intraepithelial neoplasia was significantly higher than that in low grade intraepithelial neoplasia (P < 0.05). The expression of p53 in high grade intraepithelial neoplasia of colorectal tubular adenoma may be associated with damp-heat syndrome of large intestine. The expression of P53 bFGF in colorectal tubular adenoma is significantly higher than that in normal intestinal mucosa. The expression of p53 and bFGF may play an important role in the malignant transformation of adenoma.
【学位授予单位】：福建中医药大学
【学位级别】：硕士
【学位授予年份】：2017
【分类号】：R735.34

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