Runx3、P21蛋白在隆起糜烂性胃炎及胃癌组织中的表达及其相关性研究

发布时间：2018-03-01 07:01

本文关键词： 隆起糜烂性胃炎胃癌 Runx3 P21　出处：《福建中医药大学》2017年硕士论文　论文类型：学位论文

【摘要】：目的与意义:通过检测Runx3、P21蛋白在不同分型隆起糜烂性胃炎及胃癌组织中的表达情况,分析Runx3蛋白与P21蛋白在不同胃黏膜组织中表达的相关性,探讨不同分型隆起糜烂性胃炎与胃癌的关系及可能存在的癌变分子机制,为今后隆起糜烂性胃炎的临床干预提供更多的理论依据。研究方法:收集2016年2月至2016年12月于福建中医药大学附属人民医院消化内镜科行电子胃镜检查的符合研究要求的病例,分为慢性浅表性胃炎(chronic superficial gastritis,CSG)、隆起糜烂性胃炎(raised erosive gastritis,REG)未成熟型、REG 成熟型及胃癌(gastric cancer,GC)四组,每组各30例,通过免疫组织化学染色SP法检测Runx3、P21蛋白在四组患者胃黏膜组织中的表达情况。结果:1.REG未成熟型组及REG成熟型组的萎缩发生率分别为30.0%(9/30)、43.3%(13/30),肠上皮化生(简称肠化)发生率分别为13.3%(4/30)、36.7%(11/30),上皮内瘤变发生率分别为3.3%(1/30)、13.3%(4/30),REG成熟型组肠化发生率显著高于REG未成熟型组(P0.05),而两组胃黏膜的萎缩及上皮内瘤变发生率均无显著差异(P0.05)。2.CSG组、REG未成熟型组、REG成熟型组及GC组的Runx3蛋白表达阳性率逐渐下降,分别为:80.0%(24/30)、73.3%(22/30)、43.3%(13/30)、36.7%(11/30)。REG成熟型组Runx3蛋白表达的阳性率显著低于CSG组及REG未成熟型组(P0.05),但与GC组相比,差异无统计学意义(P0.05)。CSG组Runx3蛋白表达的阳性率显著高于REG成熟型组及GC组(P0.05),但与REG未成熟型组相比,差异无统计学意义(P0.05)。3.CSG组、REG未成熟型组、REG成熟型组及GC组的P21蛋白表达阳性率分别为:70.0%(21/30)、60.0%(18/30)、36.7%(11/30)、33.3%(10/30),呈逐渐下降趋势。REG成熟型组P21蛋白表达的阳性率显著低于CSG组(P0.05),但与GC组及REG未成熟型组表达相比,差异均无统计学意义(P0.05)。CSG组P21蛋白表达的阳性率显著高于REG成熟型组及GC组(P0.05),而与REG未成熟型相比,差异无统计学意义(P0.05)。4.REG成熟型组Runx3蛋白表达与肠上皮化生有相关性,且呈负相关(r=-0.386,P0.05);REG未成熟型组Runx3蛋白表达与肠上皮化生无相关性(r=-0.207,P0.05)。5.Runx3与P21蛋白在CSG组及REG未成熟型组的表达均无相关性(r= 0.218,P0.05;r= 0.277,P0.05);Runx3与P21蛋白在REG成熟型组的表达有相关性,且呈正相关(r= 0.591,P0.05);二者在GC组的表达亦有相关性,且呈正相关(r= 0.636,P0.05)。结论:Runx3和P21蛋白在胃癌组织中表达明显下降或缺失,提示Runx3和P21蛋白可能共同参与了胃黏膜癌变的过程;Runx3和P21蛋白在成熟型隆起糜烂性胃炎组织中表达亦明显下降或缺失,提示成熟型的隆起糜烂性胃炎已经具有癌变的潜能,需要临床早期干预。
[Abstract]:Objective and significance: by detecting the expression of Runx3p21 protein in erosive gastritis and gastric carcinoma, the relationship between Runx3 protein and P21 protein expression in different gastric mucosa was analyzed. To explore the relationship between erosive gastritis and gastric cancer and the possible molecular mechanism of carcinogenesis. To provide more theoretical basis for the clinical intervention of erosive gastritis in the future. Methods: to collect the coincidence of electronic gastroscopy in the Department of Digestive Endoscopy from February 2016 to December 2016 in the people's Hospital affiliated to Fujian University of traditional Chinese Medicine. The cases required by the study, The patients were divided into four groups: chronic superficial gastritis, raised erosive gastritis, immature type and gastric cancer with 30 cases in each group. Immunohistochemistry SP method was used to detect the expression of Runx3P21 protein in gastric mucosa of four groups of patients. Results 1. The incidence of atrophy in immature group and mature group of REG was 30.010 / 30 and 13.3 / 30 respectively, intestinal metaplasia occurred in intestinal metaplasia (abbreviated as intestinal metaplasia). The incidence of intraepithelial neoplasia was 3.3 / 30, respectively, and the incidence of intestinal metaplasia in the mature type group was significantly higher than that in the immature REG group, while there was no significant difference between the two groups in the incidence of gastric mucosal atrophy and intraepithelial neoplasia. 2. There was no significant difference in the incidence of gastric mucosal atrophy and intraepithelial neoplasia between the two groups. The positive rate of Runx3 protein expression in mature group and GC group decreased gradually. The positive rate of Runx3 protein expression in 13 / 30 / 30 and 36.7% mature type group was significantly lower than that in CSG group and REG immature type group (P 0.05), but compared with GC group, the positive rate of Runx3 protein expression was significantly lower than that in CSG group and REG immature type group. The positive rate of Runx3 protein expression in Runx3 group was significantly higher than that in REG mature type group and GC group, but it was significantly higher than that in REG immature type group. The positive rate of P21 protein expression in the mature type group and GC group were 70.021 / 30 and 60.018 / 30 respectively. The positive rate of P21 protein expression in the mature type group was significantly lower than that in the CSG group, and the positive rate of P21 protein expression in the mature type group was significantly lower than that in the CSG group, and the positive rate of P21 protein expression in the mature type group was significantly lower than that in the CSG group, and the positive rate of P21 protein expression in the mature group was significantly lower than that in the CSG group, and the positive rate of P21 protein expression was significantly lower in the mature type group than in the CSG group, and the positive rate of P21 protein expression was significantly lower than that in the control group and the GC group. Compared with the expression of REG immature group, The positive rate of P21 protein expression in P0.05G group was significantly higher than that in REG mature type group and GC group, but there was no significant difference compared with REG immature type. 4. There was no correlation between the expression of P21 protein and intestinal metaplasia in mature type group. There was no correlation between the expression of Runx3 protein and intestinal metaplasia in CSG group and REG immature type group. There was no correlation between the expression of P21 protein and the expression of P21 protein in REG mature type group, and there was no correlation between the expression of P21 protein and the expression of P21 protein in the REG mature type group, and there was no correlation between the expression of P21 protein and the expression of P21 protein in the REG mature type group, and there was no correlation between the expression of P21 protein and the expression of P21 protein in the REG mature type group, and there was no correlation between the expression of P21 protein and the expression of P21 protein in the REG mature type group. There was also a positive correlation between the expression of the two proteins in GC group, and a positive correlation between them. Conclusion the expression of the protein of: Runx3 and P21 is significantly decreased or absent in gastric carcinoma. These results suggest that Runx3 and P21 proteins may be involved in the process of gastric mucosal carcinogenesis. The expression of Runx3 and P21 proteins in mature protuberance erosive gastritis is also decreased or absent, suggesting that mature protuberance erosive gastritis has the potential of carcinogenesis. Early clinical intervention is needed.
【学位授予单位】：福建中医药大学
【学位级别】：硕士
【学位授予年份】：2017
【分类号】：R735.2;R573.3

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