石蒜科植物朱顶红中生物碱类成分的分离与结构鉴定
发布时间：2018-10-15 13:56
【摘要】：朱顶红Hippeastrum vittatum(Ait.)Herb为石蒜科(Amaryllidaceae)朱顶红属(Hippeastrum),多年生草本植物,主要为栽培品种,其鳞茎入药,味辛,性温,有小毒,具有解毒消肿的功效,主治痈疮肿毒。本论文对以下三个方面进行了研究:1、本文对朱顶红及其同属植物,对其化学成分和药理作用等方面的研究进展进行了综述。2、对朱顶红鳞茎中的生物碱类成分进行了提取分离:取朱顶红鳞茎采用甲醇浸提后,减压回收甲醇,将浓缩后的水溶液经D-101大孔树脂柱洗脱,收集70%甲醇洗脱部分,减压回收甲醇,得到朱顶红鳞茎中的生物碱类粗提取物。之后采用正相硅胶柱色谱法,分离得到9个生物碱类成分:I、II、III、IV、V、VI、VI I、VIII、IX。3、通过理化性质测定及现代波谱学方法,对分离得到的生物碱类成分进行了结构鉴定,分别为:山小星蒜碱(montanine)(I),11-表山小星蒜碱(11-epimontanine)(II),8-O-去甲基高石蒜碱(8-O-demethylhomolycorenine)(III),小星蒜碱(hippeastrine)(IV)、4a-表-小星蒜碱(4a-epi-hippeastrine)(V)、石蒜碱(lycorine)(VI)、伪石蒜碱(pseudolycorine)(VII)、恩其明(ungeremine)(VIII)、8-O-demethylvasconine(IX)。其中:化合物II、V为新化合物,化合物III、VII、VI II、IX为首次从朱顶红中分离得到。
[Abstract]:Hippeastrum vittatum (Ait.) Herb is a perennial herbaceous plant of the genus (Hippeastrum), of Lycoris (Amaryllidaceae). It is mainly cultivated. Its bulb is used in medicine, taste and Xin, sex is warm, it has small poison, it has the effect of detoxification and detumescence, and it is mainly used to treat carbuncle swelling poison. In this paper, the following three aspects were studied: 1. The advances in chemical composition and pharmacological action were reviewed. 2. The alkaloids in the bulb were extracted and separated: methanol was extracted from the bulb and methanol was recovered at low pressure. The concentrated aqueous solution was eluted on D-101 macroporous resin column, 70% methanol was collected and methanol was recovered at low pressure to obtain crude alkaloid extract from red bulb. After that, nine alkaloids were isolated by normal phase silica gel column chromatography. The structures of the alkaloids were identified by physicochemical properties and modern spectroscopic methods. The results are as follows: (montanine) (I), 11- 11-epimontanine) (II), 8-O- 8-O-demethylhomolycorenine) (III), allicin (hippeastrine) (IV), 4a- 4a-epi-hippeastrine) (V), allicin (lycorine) (VI), pseudoallicin (pseudolycorine) (VII), en Keemin (ungeremine) (VIII), 8-O-demethylvasconine (IX). Among them, compound II,V is a new compound and compound III,VII,VI II,IX is isolated from red for the first time.
【学位授予单位】：吉林大学
【学位级别】：硕士
【学位授予年份】：2017
【分类号】：R284.1
本文编号：2272760
[Abstract]:Hippeastrum vittatum (Ait.) Herb is a perennial herbaceous plant of the genus (Hippeastrum), of Lycoris (Amaryllidaceae). It is mainly cultivated. Its bulb is used in medicine, taste and Xin, sex is warm, it has small poison, it has the effect of detoxification and detumescence, and it is mainly used to treat carbuncle swelling poison. In this paper, the following three aspects were studied: 1. The advances in chemical composition and pharmacological action were reviewed. 2. The alkaloids in the bulb were extracted and separated: methanol was extracted from the bulb and methanol was recovered at low pressure. The concentrated aqueous solution was eluted on D-101 macroporous resin column, 70% methanol was collected and methanol was recovered at low pressure to obtain crude alkaloid extract from red bulb. After that, nine alkaloids were isolated by normal phase silica gel column chromatography. The structures of the alkaloids were identified by physicochemical properties and modern spectroscopic methods. The results are as follows: (montanine) (I), 11- 11-epimontanine) (II), 8-O- 8-O-demethylhomolycorenine) (III), allicin (hippeastrine) (IV), 4a- 4a-epi-hippeastrine) (V), allicin (lycorine) (VI), pseudoallicin (pseudolycorine) (VII), en Keemin (ungeremine) (VIII), 8-O-demethylvasconine (IX). Among them, compound II,V is a new compound and compound III,VII,VI II,IX is isolated from red for the first time.
【学位授予单位】：吉林大学
【学位级别】：硕士
【学位授予年份】：2017
【分类号】：R284.1
【参考文献】
相关期刊论文 前1条
1 徐宏峰;王富乾;张耕;;朱顶红鳞茎生物碱类成分及抗肿瘤活性研究[J];中国药学杂志;2014年22期
，本文编号：2272760
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