基于SSR和AFLP标记的东方百合群体遗传图谱构建及QTL定位

发布时间：2017-12-28 04:22

本文关键词：基于SSR和AFLP标记的东方百合群体遗传图谱构建及QTL定位　出处：《中国农业科学院》2016年博士论文　论文类型：学位论文

【摘要】：百合属植物是单子叶植物纲百合科的多年生球根花卉,其在全世界范围内分布约有100种(变种),是植物中基因组最大的类群之一。东方百合(Oriental Hybrid lily)是百合中一个重要的杂种系,不同的百合杂种系群体得到构建,如AA(亚洲系百合×亚洲系百合),AL(亚洲系百合×铁炮百合),OT(东方系百合×喇叭百合)等。在本实验室构建了OO(东方百合×东方百合)作图群体,利用将SSR和AFLP两种分子标记法构建了百合连锁群。其中,利用本实验室开发的495对SSR引物对群体进行检测。试验中最终用于构建连锁图谱的F1群体为100个单株。利用JoinMap4软件的CP模型对获得的分子标记数据进行处理分析,构建了30个遗传连锁群图谱,其中?Sorbonne’(母本)和?Gaudi’(父本)各为12个,F1代为6个。分析观赏和农艺性状后,用MapQTL 4.0软件定位遗传连锁图谱中的QTL。主要的研究结果总结如下:1.试验对940对引物组进行了筛选,筛选出172对合适的引物组合(包括96个个AFLP标记和76个SSR标记引物)用于构建图谱。最终共标记出616个位点,其中AFLP标记出465个位点,SSR标记出151个位点。最终获得616个多态性位点,其中AFLP位点465个,SSR位点151个。为了对标记进行分组,对连锁值(LOD)进行了2到10的梯度设置。在分组后,利用LOD为3.0-5.0构建了连锁图谱。整个图谱的长度为2144.2cM.在遗传图谱中共显示189个标记位点,其中AFLP位点142个,SSR位点47个。2.母系为12个连锁群,全长为851.6cM。父本最小和最大的连锁群的遗传距离分别为2.2cM和55.7cM。父本遗传连锁图谱共有52个标记位点,其中AFLP位点42个,SSR位点10个。在12个连锁群的父本遗传图谱中,连锁群全长为676 cM,标记位51个。结果表明,全长中AFLP标记位点46个,SSR标记位点5个。在母系图谱中的最小连锁群距离为0.1 cM,而最大距离为47.7cM。在F1代的6个连锁群中发现有86完全评估位点。结果还表明,AFLP标记数得分位点为54位点,SSR标记位点有32个。F1代的连锁群总长度为606.6 cM。F1代最小和最大的连锁群的距离分别为0.2 cM和26.8 cM。3.检测到控制重要百合性状的8个QTL位点,每一个QTL位点可解释表型变异的2.4%~89.5%。在LG-F1P2上,LOD LOD的最高值的最高值(35.21)下找到了4个QTL。母系的LG-M10上的两个标记位点间定位到一个有关叶数的QTL,其LOD值为7.08。以E-CGC/M-CGC-4为引物,将花被片长度的QTL定位在F1杂交图谱的LG-F1P2上。花被片宽度的QTL与LN相似,定位在母系的LG-M10上。PW2的QTL在父本的LG-F4。在F1、母本和父本图谱上定位有5个关于斑点数量的QTL,1个长度为51cM关于斑点大小的QTL在母系的LG-M8上,以E-CGC/M-CGC-4为引物在F1的LG-F1P2上定位到株高的QTL,其LOD值为12.54。
[Abstract]:Lilium is Monocotyledoneae Liliaceae perennial bulbous flower, its distribution in the whole world there are about 100 species (varieties), is one of the largest groups in the plant genome. Oriental Lily (Oriental Hybrid lily) is one of the most important hybrid lilies in different hybrids groups are constructed, such as AA (Asian Lilium * Asian lily), AL (Asian Lilium longiflorum x), OT (Oriental Lily Lily * horn). A group of OO (Oriental Lily and Oriental Lily) was constructed in this laboratory. The Lilium chain group was constructed by two molecular markers of SSR and AFLP. Among them, 495 pairs of SSR primers developed in our laboratory were used to detect the population. In the experiment, the F1 population, which was finally used to construct the linkage map, was 100 single plants. Based on the CP model of JoinMap4 software, we processed and analyzed the obtained molecular marker data, and constructed 30 genetic linkage map. Among them, Sorbonne and Gaudi were 12 and F1 6, respectively. After analyzing the ornamental and agronomic traits, the MapQTL 4 software was used to locate the QTL in the genetic linkage map. The main results are summarized as follows: 1.. 940 pairs of primers were screened out, and 172 pairs of suitable primer combinations (including 96 AFLP markers and 76 SSR primers) were screened out for constructing map. At last, 616 loci were labeled, of which 465 loci were labeled by AFLP and 151 loci were marked by SSR. In the end, 616 polymorphic loci were obtained, including 465 AFLP loci and 151 SSR loci. In order to group the tags, a gradient setting of 2 to 10 of the chain value (LOD) is performed. After grouping, LOD was used to construct a linkage map for 3.0-5.0. The length of the entire map is 2144.2cM. in the genetic map showing 189 marker loci, including 142 AFLP loci and 47 SSR loci. The 2. matrilineal group is 12 chain groups with a total length of 851.6cM. The genetic distances of the smallest and largest paternal linkage groups were 2.2cM and 55.7cM, respectively. There are 52 marker loci in the paternal genetic linkage map, including 42 AFLP loci and 10 SSR loci. In the paternal genetic map of 12 chain groups, the total length of the linkage group was 676 cM and the marker position was 51. The results showed that there were 46 AFLP marker loci and 5 SSR marker loci in the whole length. The minimum distance of the linkage group in the maternal linkage map is 0.1 cM, and the maximum distance is 47.7cM. 86 complete evaluation sites were found in 6 F1 generation chain groups. The results also showed that the score site of AFLP markers was 54, and there were 32 SSR markers. The total length of the F1 generation chain group was 606.6 cM. The distance between the smallest and the largest group of F1 generation is 0.2 cM and 26.8 cM, respectively. 3. the 8 QTL loci for controlling the important Lily traits were detected, and each QTL locus could explain the 2.4%~89.5% of the phenotypic variation. On LG-F1P2, 4 QTL are found at the highest value (35.21) of the maximum value of the LOD LOD. The two marker loci on the maternal LG-M10 are located between the QTL of the number of leaves and the LOD value of 7.08. Using E-CGC/M-CGC-4 as primers, the QTL of the length of the perianth was located on the LG-F1P2 of the F1 cross map. The width of the perianth of the QTL is similar to that of LN, and is located on the LG-M10 of the maternal line. The QTL of PW2 is in the father's LG-F4. In F1, maternal and paternal maps, there are 5 QTL spots about the number of spots. The 1 51cM is QTL on the maternal LG-M8, with E-CGC/M-CGC-4 as primer, and QTL on the F1 LG-F1P2, the LOD value is 12.54.
【学位授予单位】：中国农业科学院
【学位级别】：博士
【学位授予年份】：2016
【分类号】：S682.29

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