茶树精油对桃蚜的杀虫活性研究
发布时间：2021-08-30 11:01
茶树精油是桃金娘科（Myrtaceae）白千层属（Melaleucal）植物互叶白千层的新鲜枝叶经水蒸汽蒸馏而得无色至淡黄色的精油,目前被广泛应用于医疗、日化等领域中,具有良好的广谱杀菌抑菌保健作用。课题组前期分析了茶树精油的主要成分,并明确了茶树精油及其主要活性成分4-松油醇对储粮害虫具有良好的杀虫活性,但其对桃蚜的杀虫作用尚未有报道。本文结合前期工作基础,研究了茶树精油及其主要成分对桃蚜的生物活性,探讨了茶树精油对桃蚜体内基因表达的影响,并基于计算机辅助药物设计技术明确了 4-松油醇与作用靶点的结合模式,通过转录组分析初步研究了桃蚜应答4-松油醇熏蒸胁迫的相关基因表达模式,为进一步研究4-松油醇对蚜虫的杀虫分子机制提供了前期基础。主要结果如下:1、茶树精油对桃蚜的驱避和触杀活性研究生物活性测定实验结果表明,茶树精油对桃蚜的驱避和触杀作用较好,且具有时间和剂量效应;浓度为2g/L茶树精油处理试虫48 h后,其对桃蚜的驱避指数（deterrence index,DI）为0.75;浓度为4g/L茶树精油处理试虫72 h后,其对桃蚜的触杀效果可达92.58%;茶树精油处理24 h对桃蚜触杀...
【文章来源】：安徽农业大学安徽省
【文章页数】：83 页
【学位级别】：博士
【文章目录】：
ACKNOWLEDGEMENTS
ABSTRACT
摘要
1 Literature review
1.1 Introduction of M. persicae
1.1.1 Pest status
1.1.2 Life cycle
1.2 Control measure
1.2.1 Chemical management
1.2.2 Essential oils
1.3 Melaleuca alternifolia oil
1.4 Justification
1.5 Objective
1.6 Significance of study
2 Insecticidal and repellent activity of M. alternifolia essential oil against M. persicae
2.1 Materials and Method
2.1.1 Bio-safety
2.1.2 Myzus persicae culture
2.1.3 Deterrent bioassay
2.1.4 Contact bioassay
2.2 Results and Discussion
2.2.1 Repellency effect
2.2.2 Contact toxicity
2.2.3 Lethal concentrations
3 Gene expression profiling of M. aternifolia essential oil against M. persicae
3.1 Materials and Methods
3.1.1 Preparation of essential oil from M. alternifolia
3.1.2 Myzus persicae culture
3.1.3 Contact toxicity/ lethal concentration
3.1.4 Real-Time Quantitative PCR (qRT-PCR)
3.1.5 Analysis
3.2 Results and Discussion
3.2.1 Gene expression of OSD gene
3.2.2 Gene expression of TOL gene
3.2.3 Gene expression of ANT gene
3.2.4 Gene expression of HSP 60
3.2.5 Gene expression of FPPS Ⅰ
4 Explore insecticidal potential of M.alternifolia essential oil against M.persicae
4.1 Computation Methods
4.1.1 Sequence analysis and modelling
4.1.2 Ligand preparation and molecular docking
4.1.3 Molecular dynamics simulations
4.1.4 Free energy calculation
4.2 Result and Discussion
4.2.1 In-silico studies
4.2.2 Structural Description of the OSD,FPPS Ⅰ,and HSP 60 3D Model
4.2.3 Molecular docking and molecular dynamics simulation
4.2.4 Molecular dynamics simulation,MMGB/BSA
5 Fumigant effects and transcriptomix analysis of terpinen-4-ol on M.persicae
5.1 Materials and Method
5.1.1 Myzus persicae culture
5.1.2 Bioassay of M.persicae
5.1.3 Insecticide exposure,RNA extraction,library preparation and sequencing
5.1.4 Bioinformatics analysis of RNA-Seq and differential expression analysis
5.2 Results and Discussion
5.2.1 Fumigant bioassay
5.2.2 Synergistic effects
5.2.3 Transcriptome sequencing analysis of M.persicae treated with terpinen-4-ol
5.2.4 Go and KEGG analysis of DEGs
6 Conclusions
7 Reference
作者简介
本文编号：3372655
【文章来源】：安徽农业大学安徽省
【文章页数】：83 页
【学位级别】：博士
【文章目录】：
ACKNOWLEDGEMENTS
ABSTRACT
摘要
1 Literature review
1.1 Introduction of M. persicae
1.1.1 Pest status
1.1.2 Life cycle
1.2 Control measure
1.2.1 Chemical management
1.2.2 Essential oils
1.3 Melaleuca alternifolia oil
1.4 Justification
1.5 Objective
1.6 Significance of study
2 Insecticidal and repellent activity of M. alternifolia essential oil against M. persicae
2.1 Materials and Method
2.1.1 Bio-safety
2.1.2 Myzus persicae culture
2.1.3 Deterrent bioassay
2.1.4 Contact bioassay
2.2 Results and Discussion
2.2.1 Repellency effect
2.2.2 Contact toxicity
2.2.3 Lethal concentrations
3 Gene expression profiling of M. aternifolia essential oil against M. persicae
3.1 Materials and Methods
3.1.1 Preparation of essential oil from M. alternifolia
3.1.2 Myzus persicae culture
3.1.3 Contact toxicity/ lethal concentration
3.1.4 Real-Time Quantitative PCR (qRT-PCR)
3.1.5 Analysis
3.2 Results and Discussion
3.2.1 Gene expression of OSD gene
3.2.2 Gene expression of TOL gene
3.2.3 Gene expression of ANT gene
3.2.4 Gene expression of HSP 60
3.2.5 Gene expression of FPPS Ⅰ
4 Explore insecticidal potential of M.alternifolia essential oil against M.persicae
4.1 Computation Methods
4.1.1 Sequence analysis and modelling
4.1.2 Ligand preparation and molecular docking
4.1.3 Molecular dynamics simulations
4.1.4 Free energy calculation
4.2 Result and Discussion
4.2.1 In-silico studies
4.2.2 Structural Description of the OSD,FPPS Ⅰ,and HSP 60 3D Model
4.2.3 Molecular docking and molecular dynamics simulation
4.2.4 Molecular dynamics simulation,MMGB/BSA
5 Fumigant effects and transcriptomix analysis of terpinen-4-ol on M.persicae
5.1 Materials and Method
5.1.1 Myzus persicae culture
5.1.2 Bioassay of M.persicae
5.1.3 Insecticide exposure,RNA extraction,library preparation and sequencing
5.1.4 Bioinformatics analysis of RNA-Seq and differential expression analysis
5.2 Results and Discussion
5.2.1 Fumigant bioassay
5.2.2 Synergistic effects
5.2.3 Transcriptome sequencing analysis of M.persicae treated with terpinen-4-ol
5.2.4 Go and KEGG analysis of DEGs
6 Conclusions
7 Reference
作者简介
本文编号：3372655
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