当归多糖对大鼠软骨细胞氧化应激保护作用的实验研究

发布时间：2018-03-07 18:23

  本文选题：当归多糖　切入点：双氧水　出处：《南京医科大学》2017年博士论文　论文类型：学位论文

【摘要】：目的:本实验主要研究当归多糖(angelica sinensis polysaccharide,ASP)在双氧水(hydrogenperoxide,H_2O_2)所诱导的大鼠软骨细胞氧化应激损伤中所起的作用,并探讨其作用机制。方法:取SD大鼠双侧膝关节软骨细胞行体外分离培养,在体外培养的软骨细胞中加入H_2O_2,建立大鼠软骨细胞氧化应激损伤模型。在体外培养的大鼠软骨细胞中加入不同浓度的ASP并与H_2O_2共培养,通过检测大鼠软骨细胞的细胞活性、细胞凋亡水平、肿瘤坏死因子-α(tumournecrosis factor-α,TNF-α)和白细胞介素-1β(interleukin-1β,IL-1β)水平、超氧化物歧化酶(superoxide dismutase,SOD)和过氧化氢酶(catalase,CAT)活性以及丙二醛(malondialdehyde,MDA)水平来评价ASP的保护作用。同时应用实时定量逆转录聚合酶链反应(quantitative real-time reverse transcription polymerase chain reaction,qRT-PCR)来估测骨性关节炎(osteoarthritis,OA)相关基因的相对表达水平,其中包括II型胶原(typeⅡcollagen,Col2a1)、aggrecan、SOX9、基质金属蛋白酶(matrix metalloproteinase,MMP)-1,-3,-9以及组织基质金属蛋白酶抑制剂(tissue inhibitor of matrix metalloproteinase,TIMP)-1。结果:在H_2O_2所诱导的大鼠软骨细胞氧化应激损伤中,ASP通过其抗氧化、抗凋亡以及抗炎作用机制在体外对大鼠软骨细胞氧化应激损伤起保护作用,使大鼠软骨细胞免于氧化应激所致细胞损伤。结论:ASP可作为一种替代性药物在骨性关节炎的临床治疗中起到一定作用。
[Abstract]:Objective: to investigate the role of Angelica sinensis polysaccharide in oxidative stress injury of chondrocytes in rats induced by hydrogen peroxide peroxide (H _ 2O _ 2). Methods: the chondrocytes of bilateral knee joint of SD rats were isolated and cultured in vitro. The oxidative stress injury model of rat chondrocytes was established by adding H _ S _ 2O _ 2 into the chondrocytes cultured in vitro. Different concentrations of ASP were added to the chondrocytes cultured in vitro and co-cultured with H _ 2O _ 2 to detect the cellular activity of rat chondrocytes. The levels of apoptosis, tumor necrosis factor- 伪 (TNF- 伪) and interleukin-1 尾 interleukin-1 尾 (IL-1 尾) were measured. The activity of superoxide dismutase (SOD) and catalase (catalase) and the level of malondialdehyde (MDA) were used to evaluate the protective effect of ASP. The real-time quantitative reverse transcriptase polymerase chain reaction (RT-PCR) was used to estimate the osteoarthritis of osteoarthritis by quantitative real-time transcription polymerase chain reactionation (qRT-PCR). The relative level of expression of the related gene, These include type II collagen type 鈪，

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