基于食物过敏的傣药“雅解沙把”解“食物毒”作用机制研究
发布时间：2018-09-08 18:55
【摘要】：目的傣医解"食物毒"是"雅解"理论的重要组成部分,近年来,在"雅解"理论指导下的解"食物毒"及其方药一直是傣医药领域研究的重点和热点。研究发现"食物毒"与食物过敏密切相关;"雅解沙把"是解"食物毒"方药之一,临床观察及实验研究均证实其有较好的解"食物毒"作用,但目前研究尚不能全面揭示其解"食物毒"作用机理。故在前期研究的基础上,本研究拟达到以下目的:1运用统计学方法对傣医解"食物毒"单、验方的规律进行分析,以期证实傣医"食物毒"与食物过敏有相关性,食物过敏是最能体现"食物毒"内涵的疾病之一;"雅解沙把"是解"食物毒"代表方;肠道是研究解"食物毒"的角度或途径,从而为开展实验研究及临床运用提供依据。2根据上述理论研究,基于食物过敏小鼠模型,从肠黏膜屏障的生物屏障、物理屏障、免疫屏障为切入点研究傣药"雅解沙把"抗食物过敏可能的作用机制,从而揭示其解"食物毒"的作用机制。方法1理论研究运用文献分析法收集解"食物毒"相关"雅解"单方、验方,并进行规范化整理。运用频数分析法对其使用的药物进行统计分析,分析总结其类别、性、味、归塔,主治类别、主治范围及主治症状等方面的频数结构及分布情况。在对药物进行频数分析的基础上,运用聚类分析法对药物类别进行聚类分析。2实验研究2.1复制卵清蛋白过敏小鼠模型40只BALB/c雌鼠,6-8周龄,随机分为正常对照组、OVA模型组、"雅解沙把"低剂量组(0.39g/kg)、"雅解沙把"中剂量组(1.17g/kg)、"雅解沙把"高剂量组(3.51g/kg)。小鼠于实验第0天腹腔注射致敏液(10μgOVA加1mg氢氧化铝),第14天腹腔注射致敏液(含10μgOVA),第30天、34天灌胃致敏液(含80mgOVA)。正常对照组用超纯水代替进行致敏和激发。2.2药物干预实验在实验第30天-34天予OVA灌胃激发前1小时,对药物干预组小鼠以灌胃方式给予"雅解沙把"低、中、高剂量治疗;正常对照组和模型组小鼠以灌胃方式给予生理盐水治疗,共5天。2.3 卵清蛋白过敏模型评价指标检测在两次OVA灌胃激发后1小时内,观察各组小鼠过敏症状、腹泻情况。在末次OVA灌胃激发1小时后,取血清标本,采用酶联免疫吸附试验法(ELISA)检测特异性OVA-IgE水平。2.4 肠道菌群结构检测实验末取小鼠粪便液氮中冻存,利用Illumina高通量测序平台对各组小鼠粪便标本中的16S rRNA gene V3-V4区进行定性分析,探索小鼠肠道菌群结构的变化。2.5 肠黏膜通透性检测采用荧光定量PCR方法检测小鼠空肠紧密连接蛋白Occludin mRNA、Claudin 3 mRNA的含量。2.6 肠黏膜免疫检测采用酶联免疫吸附试验法(ELISA)检测血清IL-4、IFN-γ、IL-4/IFN-γ及小鼠肠黏膜sIgA水平;荧光定量PCR方法检测小鼠空肠IL-17、TGF-β1、TGF-β1/IL-17mRNA的含量。结果1 解"食物毒"方药物规律分析解"食物毒"相关方药物中,共收集到57首258味次、115味单方、验方。通过频次分析得出,药物类别以解药类为主,占45.74%;药物使用频率超过或等于2次的药物共计48味、占总味数的41.74%;药性主要以凉、寒为主,占比分别为:40.31%和20.54%;药味主要以苦味为主,占45.35%;药物入塔主要以风塔为主,占29.20%。"食物毒"类别以误食毒物及食物中毒为主,占35.27%和31.40%;主治范围以消化系统为主,占54.64%。主治症状主要以腹泻、呕吐为主,各占18.06%。通过频数分析与聚类分析,解"食物毒"药物可归为7类药物组合方,其中相类方"雅解沙把"符合安全性、可操作性、可获取性等临床应用推广条件。2 卵清蛋白过敏小鼠模型评价指标结果2.1 过敏症状第一次OVA灌胃激发后,"雅解沙把"低、中、高剂量组小鼠过敏症状分数较模型组均降低,但差异无统计学意义(P0.05)。第二次OVA灌胃激发后,"雅解沙把"低、中、高剂量组小鼠过敏症状分数较模型组明显降低,差异有统计学意义(P0.01)。2.2腹泻症状第一次OVA灌胃激发后,"雅解沙把"低、中、高剂量组小鼠腹泻分数较模型组均降低,但只有中剂量组有显著性差异(P0.05)。第二次OVA灌胃激发后,药物干预组小鼠腹泻分数较模型组均明显降低,差异有统计学意义(P0.01)。2.3血清OVA-IgE水平药物干预组小鼠血清OVA-IgE水平较模型组均降低,差异均有统计学意义(P0.05)。3肠道菌群结构结果3.1小鼠粪便菌群Alpha多样性及和丰富度药物干预组小鼠肠道总微生物群落丰度指标ACE、Chao1指数较模型组无差异(P0.05),但"雅解沙把"高剂量有增加两者的趋势。同时药物干预组小鼠Shannon指数较模型组有升高趋势;而Simpson指数有下降趋势,但只有中剂量组有统计学意义(P0.05)。3.2小鼠肠道微生态构成及分布丰度在"门"水平上,与模型组相比,药物干预组小鼠厚壁菌门细菌比例呈减少趋势,以高剂量组明显,差异有统计学意义(P0.05);而拟杆菌门细菌比例呈增加趋势,以高剂量组明显,差异有统计学意义(P0.05)。在"属" 水平上,与模型组相比,乳酸杆菌(Lactobacillus)属细菌呈减少趋势,但差异无统计学意义(P0.05);而Barnesiella属细菌呈增加趋势,以高剂量显著,差异有统计学意义(P0.05)。3.3肠道微生物群落结构差别与模型组相比,药物干预组小鼠微生物群落样品在空间分布上相对独立,能明显分开。4肠黏膜通透性结果与模型组相比,药物干预组小鼠肠上皮紧密连接蛋白Occludin mRNA表达量明显增高,差异均有统计学意义(P0.05);而肠上皮紧密连接蛋白Claudin3mRNA表达量无明显变化,无统计学意义(P0.05)。5肠黏膜免疫结果与模型组相比,"雅解沙把"低、中剂量组小鼠血清IL-4浓度有升高趋势,但无统计学差异(P0.05),而"雅解沙把"高剂量组小鼠血清IL-4浓度明显降低,差异有统计学意义(P0.05)。与模型组相比,药物干预组小鼠血清IFN-γ浓度明显升高,差异有统计学意义(P0.01)。药物干预组均有下调小鼠血清IL-4/IFN-γ比值的趋势,但只有高剂量比值显著降低,差异统计学意义(P0.05)。与模型组相比,药物干预组小鼠IL-17mRNA表达量、TGF-β1mRNA表达量、TGF-β1/IL-17比值及sIgA水平均无差异(P0.05)。结论1 解"食物毒"方药规律以解药类药物为主;药性以凉为主,药味以苦味为主;配伍以复方为主,遵循寒热并用,调平"四塔"原则。2 傣医部分"食物毒"与食物过敏有相关性,食物过敏是最能体现"食物毒"内涵的疾病之一。"雅解沙把"是解"食物毒"代表方之一,肠道是研究的角度和途径,腹泻是"食物毒"的主要症状之一。3 "雅解沙把"通过降低过敏症状分数、腹泻分数、血清OVA-IgE水平,缓解卵清蛋白过敏小鼠症状。4 "雅解沙把"通过升高小鼠Shannon指数,降低Simpson指数,增加菌落多样性,调节失衡的肠道菌群。5 在肠道微生态的门水平上,"雅解沙把"能降低小鼠厚壁菌门细菌比例,增加拟杆菌门细菌比例,恢复失衡的肠道菌群,且以"雅解沙把"高剂量效果显著。6 在肠道微生态的属水平上,"雅解沙把"能降低小鼠乳酸杆菌属细菌比例,增加Barnesiella属细菌比例,恢复失衡的肠道菌群,且以"雅解沙把"高剂量效果显著。7 "雅解沙把"可以恢复小鼠肠道微生物群落结构差别。8 "雅解沙把"通过提高小鼠Occludin mRNA的含量,保护肠黏膜上皮的完整性,降低通透性,其对Claudin 3 mRNA的含量无影响。9 "雅解沙把"通过降低小鼠血清IL-4浓度,升高IFN-γ浓度,下调IL-4/IFN-γ比值,可对免疫细胞Th2优势所致的免疫失衡状态进行调节,恢复Th1/Th2平衡。10 "雅解沙把"对 IL-17 mRNA、TGF-β1 mRNA 表达量,TGF-β1/IL-17 比值及 sIgA水平无影响。11 "雅解沙把"治疗食物过敏具有应用前景,尤其是高剂量效果显著。
[Abstract]:OBJECTIVE To understand food poisoning in Dai medicine is an important part of Yajie theory. In recent years, it has been the focus and hotspot in the field of Dai medicine to solve food poisoning and its prescriptions under the guidance of Yajie theory. It has been proved that it has a good effect on "food poisoning", but the mechanism of "food poisoning" can not be fully revealed in the present study. Therefore, on the basis of previous studies, this study intends to achieve the following purposes: 1. Using statistical methods to analyze the rules of Dai medicine's "food poisoning" list and prescriptions, in order to confirm the Dai medicine's "food poisoning" and food allergy. Relatively, food allergy is one of the diseases that can best reflect the connotation of "food toxicity"; Yajiesha Ba is the representative prescription of "food toxicity"; intestinal tract is the angle or way to study the understanding of "food toxicity", so as to provide the basis for experimental research and clinical application. Object barrier, physical barrier, immune barrier as the breakthrough point to study the possible mechanism of Dai medicine "Yajieshaba" anti-food allergy, so as to reveal the mechanism of its anti-food toxicity. Methods 1 Theoretical research using literature analysis method to collect the "Yajie" related to "food toxicity" single, test prescriptions, and standardize. The frequency structure and distribution of the drugs were analyzed and summarized in terms of category, sex, taste, attribution, main treatment category, main treatment range and main symptoms. 0 female BALB/c mice aged 6-8 weeks were randomly divided into normal control group, OVA model group, low dose group (0.39 g/kg), middle dose group (1.17 g/kg) and high dose group (3.51 g/kg). The mice were intraperitoneally injected with sensitizing solution (10 mg OVA plus 1 mg aluminium hydroxide) on day 0, day 14 and day 30 respectively. The normal control group was given super pure water instead of sensitizing and stimulating. 2.2 Drug intervention experiment was given OVA 1 hour before stimulating by gavage on the 30th and 34th days of the experiment. The mice in the drug intervention group were given Yajieshabao by gavage at low, medium and high doses; the mice in the normal control group and the model group were given Yajieshabao by gavage. The allergic symptoms and diarrhea of mice in each group were observed within 1 hour after two stimulations of OVA. After the last stimulation of OVA, serum samples were taken and the specific OVA-IgE level was detected by enzyme linked immunosorbent assay (ELISA). The 16S rRNA gene V3-V4 region was analyzed qualitatively by Illumina high-throughput sequencing platform to explore the changes of intestinal flora structure in mice. 2.5 Intestinal mucosal permeability was detected by fluorescence quantitative PCR to detect the tight junction protein Occludin m in mouse jejunum. Enzyme-linked immunosorbent assay (ELISA) was used to detect the levels of serum IL-4, IFN-gamma, IL-4/IFN-gamma and sIgA in mouse intestinal mucosa; fluorescence quantitative PCR was used to detect the contents of IL-17, TGF-beta 1, TGF-beta 1/IL-17 mRNA in mouse jejunum. Among the prescriptions, 57 258 were collected, 115 were single, and the prescriptions were tested. Through frequency analysis, it was concluded that antidotes were the main types of drugs, accounting for 45.74%; 48 drugs were used more frequently than or equal to two times, accounting for 41.74%; medicinal properties were mainly cold, accounting for 40.31% and 20.54% respectively; bitterness was the main flavor. The main types of food poisoning were mistaken poisons and food poisoning, accounting for 35.27% and 31.40%; the main treatment areas were digestive system, accounting for 54.64%. The main symptoms were diarrhea and vomiting, accounting for 18.06% respectively. 2. Evaluation index of ovalbumin allergic mice model 2. 1 Allergic symptoms after the first OVA stimulation, the "Yajieshaba" was low, the middle and high dose group of mice allergic symptoms were lower than the model group, but the difference was not unified. The scores of allergic symptoms in the middle and high dose groups were significantly lower than those in the model group (P 0.01). 2.2 After the first OVA stimulation of diarrhea symptoms, the scores of diarrhea in the middle and high dose groups were lower than those in the model group, but only in the middle dose group. There was a significant difference between the two groups (P 0.05). After the second OVA stimulation, the diarrhea fraction of the drug intervention group was significantly lower than that of the model group (P 0.01). 2.3 The serum OVA-IgE level of the drug intervention group was significantly lower than that of the model group (P 0.05). 3 The intestinal flora structure of the drug intervention group was significantly lower than that of the model group (P 0.05). The diversity and richness of Alpha microflora in feces of mice were similar to those of the model group (P 0.05), but the high dosage of Yajieshabao increased both of them. But only the middle dose group had statistical significance (P 0.05). 3.2 mice intestinal microecological composition and distribution of abundance in the "gate" level, compared with the model group, the drug intervention group mice sclerenchyma bacterial proportion showed a decreasing trend, the high dose group was obvious, the difference was statistically significant (P 0.05); and the proportion of Bacteroides bacteria showed an increasing trend, with high dose. There was significant difference between the two groups (P 0.05). Compared with the model group, the number of Lactobacillus bacteria decreased, but the difference was not statistically significant (P 0.05), while the number of Barnesiella bacteria increased, with significant difference in high dose (P 0.05). Compared with the model group, the intestinal mucosal permeability of the drug intervention group was significantly higher than that of the model group, and the expression of Occludin mRNA in the intestinal epithelium of the drug intervention group was significantly higher than that of the model group (P 0.05). There was no significant difference in the expression of in3 mRNA between the two groups (P 0.05). 5 Compared with the model group, the immunological results of intestinal mucosa were lower. The serum IL-4 concentration of the middle dose group was higher, but there was no significant difference (P 0.05). The serum IL-4 concentration of the high dose group was significantly lower than that of the model group (P 0.05). Compared with the model group, the serum IFN-gamma concentration of the drug intervention group was significantly higher, the difference was statistically significant (P 0.01). The drug intervention group had a tendency to lower the serum IL-4/IFN-gamma ratio, but only the high dose ratio was significantly lower (P 0.05). Compared with the model group, the expression of IL-17 mRNA and TGF-beta 1 mRNA in the drug intervention group were significantly lower. There was no significant difference in the expression of TGF-1/IL-17 and sIgA levels (P 0.05). Conclusion 1 The antidote drugs were the main prescriptions for "food poisoning"; the main medicinal properties were cooling, and the main flavor was bitterness; the main compatibility was compound prescriptions, followed the principle of "four towers" and "cold and heat" were balanced. "Yajieshaba" is one of the representative prescriptions of "food poisoning". Intestinal tract is the angle and way of study. Diarrhea is one of the main symptoms of "food poisoning". 3 "Yajieshaba" alleviates the symptoms of ovalbumin-allergic mice by reducing the allergic symptoms, diarrhea, serum OVA-IgE levels. Over-elevating Shannon index, reducing Simpson index, increasing colony diversity and regulating the imbalance of intestinal flora. 5 At the gate level of intestinal microecology, "Yajiesha Bag" can reduce the proportion of sclerenchyma bacteria in mice, increase the proportion of Bacteroides bacteria, restore the imbalance of intestinal flora, and the high dose of "Yajiesha Bat" has a significant effect in the intestine. At the genus level of Tao micro-ecology, Yajieshaba could reduce the proportion of Lactobacillus, increase the proportion of Barnesiella, restore the unbalanced intestinal flora, and the effect of high dose Yajieshaba was significant. 7 Yajieshaba could restore the difference of intestinal microbial community structure in mice. 8 Yajieshaba could increase the Occludin of mice by increasing the proportion of Barnesiella. The content of mRNA, the integrity of intestinal mucosa epithelium and the permeability of intestinal mucosa were protected, but the content of Claudin 3 mRNA was not affected. 9 "Yajieshabao" could regulate the immune imbalance caused by Th2 dominance of immune cells and restore the Th1/Th2 balance by lowering serum IL-4 concentration, increasing IFN-gamma concentration and decreasing the ratio of IL-4 to IFN-gamma in mice. "No effect on the expression of IL-17 mRNA, TGF-beta 1 mRNA, TGF-beta 1/IL-17 ratio and sIgA level was observed. 11" Yajiesha "has a bright future in the treatment of food allergy, especially in high dosage.
【学位授予单位】：北京中医药大学
【学位级别】：博士
【学位授予年份】：2017
【分类号】：R29
本文编号：2231396
[Abstract]:OBJECTIVE To understand food poisoning in Dai medicine is an important part of Yajie theory. In recent years, it has been the focus and hotspot in the field of Dai medicine to solve food poisoning and its prescriptions under the guidance of Yajie theory. It has been proved that it has a good effect on "food poisoning", but the mechanism of "food poisoning" can not be fully revealed in the present study. Therefore, on the basis of previous studies, this study intends to achieve the following purposes: 1. Using statistical methods to analyze the rules of Dai medicine's "food poisoning" list and prescriptions, in order to confirm the Dai medicine's "food poisoning" and food allergy. Relatively, food allergy is one of the diseases that can best reflect the connotation of "food toxicity"; Yajiesha Ba is the representative prescription of "food toxicity"; intestinal tract is the angle or way to study the understanding of "food toxicity", so as to provide the basis for experimental research and clinical application. Object barrier, physical barrier, immune barrier as the breakthrough point to study the possible mechanism of Dai medicine "Yajieshaba" anti-food allergy, so as to reveal the mechanism of its anti-food toxicity. Methods 1 Theoretical research using literature analysis method to collect the "Yajie" related to "food toxicity" single, test prescriptions, and standardize. The frequency structure and distribution of the drugs were analyzed and summarized in terms of category, sex, taste, attribution, main treatment category, main treatment range and main symptoms. 0 female BALB/c mice aged 6-8 weeks were randomly divided into normal control group, OVA model group, low dose group (0.39 g/kg), middle dose group (1.17 g/kg) and high dose group (3.51 g/kg). The mice were intraperitoneally injected with sensitizing solution (10 mg OVA plus 1 mg aluminium hydroxide) on day 0, day 14 and day 30 respectively. The normal control group was given super pure water instead of sensitizing and stimulating. 2.2 Drug intervention experiment was given OVA 1 hour before stimulating by gavage on the 30th and 34th days of the experiment. The mice in the drug intervention group were given Yajieshabao by gavage at low, medium and high doses; the mice in the normal control group and the model group were given Yajieshabao by gavage. The allergic symptoms and diarrhea of mice in each group were observed within 1 hour after two stimulations of OVA. After the last stimulation of OVA, serum samples were taken and the specific OVA-IgE level was detected by enzyme linked immunosorbent assay (ELISA). The 16S rRNA gene V3-V4 region was analyzed qualitatively by Illumina high-throughput sequencing platform to explore the changes of intestinal flora structure in mice. 2.5 Intestinal mucosal permeability was detected by fluorescence quantitative PCR to detect the tight junction protein Occludin m in mouse jejunum. Enzyme-linked immunosorbent assay (ELISA) was used to detect the levels of serum IL-4, IFN-gamma, IL-4/IFN-gamma and sIgA in mouse intestinal mucosa; fluorescence quantitative PCR was used to detect the contents of IL-17, TGF-beta 1, TGF-beta 1/IL-17 mRNA in mouse jejunum. Among the prescriptions, 57 258 were collected, 115 were single, and the prescriptions were tested. Through frequency analysis, it was concluded that antidotes were the main types of drugs, accounting for 45.74%; 48 drugs were used more frequently than or equal to two times, accounting for 41.74%; medicinal properties were mainly cold, accounting for 40.31% and 20.54% respectively; bitterness was the main flavor. The main types of food poisoning were mistaken poisons and food poisoning, accounting for 35.27% and 31.40%; the main treatment areas were digestive system, accounting for 54.64%. The main symptoms were diarrhea and vomiting, accounting for 18.06% respectively. 2. Evaluation index of ovalbumin allergic mice model 2. 1 Allergic symptoms after the first OVA stimulation, the "Yajieshaba" was low, the middle and high dose group of mice allergic symptoms were lower than the model group, but the difference was not unified. The scores of allergic symptoms in the middle and high dose groups were significantly lower than those in the model group (P 0.01). 2.2 After the first OVA stimulation of diarrhea symptoms, the scores of diarrhea in the middle and high dose groups were lower than those in the model group, but only in the middle dose group. There was a significant difference between the two groups (P 0.05). After the second OVA stimulation, the diarrhea fraction of the drug intervention group was significantly lower than that of the model group (P 0.01). 2.3 The serum OVA-IgE level of the drug intervention group was significantly lower than that of the model group (P 0.05). 3 The intestinal flora structure of the drug intervention group was significantly lower than that of the model group (P 0.05). The diversity and richness of Alpha microflora in feces of mice were similar to those of the model group (P 0.05), but the high dosage of Yajieshabao increased both of them. But only the middle dose group had statistical significance (P 0.05). 3.2 mice intestinal microecological composition and distribution of abundance in the "gate" level, compared with the model group, the drug intervention group mice sclerenchyma bacterial proportion showed a decreasing trend, the high dose group was obvious, the difference was statistically significant (P 0.05); and the proportion of Bacteroides bacteria showed an increasing trend, with high dose. There was significant difference between the two groups (P 0.05). Compared with the model group, the number of Lactobacillus bacteria decreased, but the difference was not statistically significant (P 0.05), while the number of Barnesiella bacteria increased, with significant difference in high dose (P 0.05). Compared with the model group, the intestinal mucosal permeability of the drug intervention group was significantly higher than that of the model group, and the expression of Occludin mRNA in the intestinal epithelium of the drug intervention group was significantly higher than that of the model group (P 0.05). There was no significant difference in the expression of in3 mRNA between the two groups (P 0.05). 5 Compared with the model group, the immunological results of intestinal mucosa were lower. The serum IL-4 concentration of the middle dose group was higher, but there was no significant difference (P 0.05). The serum IL-4 concentration of the high dose group was significantly lower than that of the model group (P 0.05). Compared with the model group, the serum IFN-gamma concentration of the drug intervention group was significantly higher, the difference was statistically significant (P 0.01). The drug intervention group had a tendency to lower the serum IL-4/IFN-gamma ratio, but only the high dose ratio was significantly lower (P 0.05). Compared with the model group, the expression of IL-17 mRNA and TGF-beta 1 mRNA in the drug intervention group were significantly lower. There was no significant difference in the expression of TGF-1/IL-17 and sIgA levels (P 0.05). Conclusion 1 The antidote drugs were the main prescriptions for "food poisoning"; the main medicinal properties were cooling, and the main flavor was bitterness; the main compatibility was compound prescriptions, followed the principle of "four towers" and "cold and heat" were balanced. "Yajieshaba" is one of the representative prescriptions of "food poisoning". Intestinal tract is the angle and way of study. Diarrhea is one of the main symptoms of "food poisoning". 3 "Yajieshaba" alleviates the symptoms of ovalbumin-allergic mice by reducing the allergic symptoms, diarrhea, serum OVA-IgE levels. Over-elevating Shannon index, reducing Simpson index, increasing colony diversity and regulating the imbalance of intestinal flora. 5 At the gate level of intestinal microecology, "Yajiesha Bag" can reduce the proportion of sclerenchyma bacteria in mice, increase the proportion of Bacteroides bacteria, restore the imbalance of intestinal flora, and the high dose of "Yajiesha Bat" has a significant effect in the intestine. At the genus level of Tao micro-ecology, Yajieshaba could reduce the proportion of Lactobacillus, increase the proportion of Barnesiella, restore the unbalanced intestinal flora, and the effect of high dose Yajieshaba was significant. 7 Yajieshaba could restore the difference of intestinal microbial community structure in mice. 8 Yajieshaba could increase the Occludin of mice by increasing the proportion of Barnesiella. The content of mRNA, the integrity of intestinal mucosa epithelium and the permeability of intestinal mucosa were protected, but the content of Claudin 3 mRNA was not affected. 9 "Yajieshabao" could regulate the immune imbalance caused by Th2 dominance of immune cells and restore the Th1/Th2 balance by lowering serum IL-4 concentration, increasing IFN-gamma concentration and decreasing the ratio of IL-4 to IFN-gamma in mice. "No effect on the expression of IL-17 mRNA, TGF-beta 1 mRNA, TGF-beta 1/IL-17 ratio and sIgA level was observed. 11" Yajiesha "has a bright future in the treatment of food allergy, especially in high dosage.
【学位授予单位】：北京中医药大学
【学位级别】：博士
【学位授予年份】：2017
【分类号】：R29
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1 李琼超;基于食物过敏的傣药“雅解沙把”解“食物毒”作用机制研究[D];北京中医药大学;2017年
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