人参皂苷Rg3抑制人骨肉瘤细胞增殖及其分子机制研究
发布时间：2021-07-10 17:14
研究背景:骨肉瘤是发源于间充质干细胞的恶性肿瘤。骨肉瘤的发病年龄呈现双峰分布,常发病于青少年或老年人。由于大多数的骨肉瘤患者在诊断时时就有转移或微转移性,故几乎所有患者在手术切除的之前或之后都会接受多种药物联合化疗。患者往往耐受度较差,且复发几率较高。人参是一种原产于东北亚的草本植物,在东亚地区的传统医学中有着广泛的应用。作为人参中主要的活性物质之一,人参皂苷Rg3的抗肿瘤作用在近年来越来越引起人们的重视。人参皂苷Rg3的甾体结构使得它可以与细胞膜、膜结合离子通道、以及胞内和胞外的受体相互作用来改变细胞的转录水平以来发挥抗肿瘤作用。研究方法:本实验应用MTT实验、流式细胞术、细胞划痕实验、Transwell细胞侵袭实验检测了人参皂苷Rg3对骨肉瘤细胞系MG-63、HOS和U2OS增殖、凋亡、迁移、侵袭的影响和对细胞周期作用。我们又进一步选择MG-63细胞作为研究对象,应用western blot、定量PCR、流式细胞术、小干扰RNA等实验手段进一步探究人参皂苷Rg3诱导细胞自噬、凋亡以及细胞周期阻滞的具体作用机制。实验结果:MTT实验发现人参皂苷Rg3以剂量依赖的方式抑制三种骨肉瘤细胞...
【文章来源】：吉林大学吉林省 211工程院校 985工程院校 教育部直属院校
【文章页数】：110 页
【学位级别】：博士
【部分图文】：
人参皂苷Rg3的分子结构
28胞中，人参皂苷在160-1280nM的浓度范围内，可以显著抑制MG-63细胞的生长，1280nM时抑制作用最强。三种细胞系在24、48和72h时的抑制作用相近，如图2.1-3所示。图2.1MTT法检测人参皂苷Rg3对MG-63细胞增殖的抑制作用Fig2.1MTTassayshowedtheinhibitoryeffectofginsenosideRg3ontheproliferationosteosarcomaMG-63cells
29图2.2MTT法检测人参皂苷Rg3对HOS细胞增殖的抑制作用Fig2.2MTTassayshowedtheinhibitoryeffectofginsenosideRg3ontheproliferationofosteosarcomaHOScells图2.3MTT法检测人参皂苷Rg3对U2OS细胞增殖的抑制作用Fig2.MTTassayshowedtheinhibitoryeffectofginsenosideRg3ontheproliferationofosteosarcomaU2OScells[
【参考文献】：
期刊论文
[1]Ginsenoside Rg3 inhibit hepatocellular carcinoma growth via intrinsic apoptotic pathway[J]. Jian-Wen Jiang,Xin-Mei Chen,Xin-Hua Chen,Shu-Sen ZhengJian-Wen Jiang,Xin-Hua Chen,Shu-Sen Zheng,The Key Lab of Combined Multi-organ Transplantation,Ministry of Public Health,The Department of Hepatobiliary and Pancreatic Surgery,First Affiliated Hospital,School of Medicine,Zhejiang University,Hangzhou 310003,Zhejiang Province,China Xin-Mei Chen,The Department of Pharmacy,Shandong Traditional Chinese Medicine University,Jinan 250355,Shandong Province,China. World Journal of Gastroenterology. 2011(31)
[2]Inhibitory effect of ginsenoside Rg3 combined with cyclophosphamide on growth and angiogenesis of ovarian cancer[J]. XU Tian-min XIN Ying CUI Man-hua JIANG Xin GU Li-ping Department of Obstetrics and Gynaecology,Second Hospital of Jilin University,Changchun 130041,China Key Laboratory of Pathobiology,Ministry of Education,Jilin University,Changchun 130021,China Department of Radiotherapy,Second Hospital of Jilin University,Changchun 130041,China. Chinese Medical Journal. 2007(07)
本文编号：3276336
【文章来源】：吉林大学吉林省 211工程院校 985工程院校 教育部直属院校
【文章页数】：110 页
【学位级别】：博士
【部分图文】：
人参皂苷Rg3的分子结构
28胞中，人参皂苷在160-1280nM的浓度范围内，可以显著抑制MG-63细胞的生长，1280nM时抑制作用最强。三种细胞系在24、48和72h时的抑制作用相近，如图2.1-3所示。图2.1MTT法检测人参皂苷Rg3对MG-63细胞增殖的抑制作用Fig2.1MTTassayshowedtheinhibitoryeffectofginsenosideRg3ontheproliferationosteosarcomaMG-63cells
29图2.2MTT法检测人参皂苷Rg3对HOS细胞增殖的抑制作用Fig2.2MTTassayshowedtheinhibitoryeffectofginsenosideRg3ontheproliferationofosteosarcomaHOScells图2.3MTT法检测人参皂苷Rg3对U2OS细胞增殖的抑制作用Fig2.MTTassayshowedtheinhibitoryeffectofginsenosideRg3ontheproliferationofosteosarcomaU2OScells[
【参考文献】：
期刊论文
[1]Ginsenoside Rg3 inhibit hepatocellular carcinoma growth via intrinsic apoptotic pathway[J]. Jian-Wen Jiang,Xin-Mei Chen,Xin-Hua Chen,Shu-Sen ZhengJian-Wen Jiang,Xin-Hua Chen,Shu-Sen Zheng,The Key Lab of Combined Multi-organ Transplantation,Ministry of Public Health,The Department of Hepatobiliary and Pancreatic Surgery,First Affiliated Hospital,School of Medicine,Zhejiang University,Hangzhou 310003,Zhejiang Province,China Xin-Mei Chen,The Department of Pharmacy,Shandong Traditional Chinese Medicine University,Jinan 250355,Shandong Province,China. World Journal of Gastroenterology. 2011(31)
[2]Inhibitory effect of ginsenoside Rg3 combined with cyclophosphamide on growth and angiogenesis of ovarian cancer[J]. XU Tian-min XIN Ying CUI Man-hua JIANG Xin GU Li-ping Department of Obstetrics and Gynaecology,Second Hospital of Jilin University,Changchun 130041,China Key Laboratory of Pathobiology,Ministry of Education,Jilin University,Changchun 130021,China Department of Radiotherapy,Second Hospital of Jilin University,Changchun 130041,China. Chinese Medical Journal. 2007(07)
本文编号：3276336
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