阻抗谱用于骨愈合动态监测的研究

发布时间：2021-07-22 03:18

　　患有肌肉骨骼系统疾病和骨组织损伤的病人数量在逐年增加。在美国,占外伤病人总数的三分之二,每年约有630万人骨折,超过50万人需要进行骨移植。创伤性损伤造成的骨组织大量丢失为骨组织重建带来了巨大的临床挑战。在这些损伤中,严重的骨缺损特别难以修复,通常需要二次手术,最终导致骨组织不愈合。目前对于复杂骨折治疗常用的方法为利用自体髂嵴骨骺游离移植进行重建。但是该技术受限于可用于自体髂嵴骨骺数量有限,且会给患者本身带来巨大的疼痛进而造成愈合的难度。异体移植也常用于骨组织损伤治疗,但移植的条件相对于自体移植来说失败的风险随着患病时间也显著升高,并伴随着疾病传播的风险。另外,异种移植物在移植前必须经过灭菌过程,会导致多种骨诱导因子在移植材料内的失活,进而丧失功能。同时为了提高骨愈合,髓样棒和用于内部固定的金属设备有时也是必须的。然而,这些永久性的植入物由于是金属故不会被患者吸收融合,并且在长期使用期间容易发生疲劳性骨折,将这种金属器件与相邻的骨头结合是极具挑战性的。目前研究结果显示,利用生物陶瓷以及在植入物表面进行矿化涂层可以显著的提高与组织的相容性,但这些表面调节技术会导致植入装置的耐久性的下降。... 

【文章来源】：哈尔滨工业大学黑龙江省 211工程院校 985工程院校

【文章页数】：103 页

【学位级别】：博士

【文章目录】：
Abstract (In Chinese)
Abstract (In English)
Chapter 1 Introduction
    1.1 Background and significance of the subject
    1.2 Description of bone fracture healing process
    1.3 Development of EIS
        1.3.1 Theory of conducting EIS measurements of living tissues
        1.3.2 EIS Characteristics of Bone Tissue and Current Applications of EIS in the Study of Bone Tissue
    1.4 Research contents and technical route of this subject
        1.4.1 Main research contents
        1.4.2 Technical route
Chapter 2 Materials and methods
    2.1 Materials
        2.1.1 Animals and cell lines
        2.1.2 Regents and equipments
        2.1.3 Web site and software
    2.2 Methods
        2.2.1 Apparatus for electric impedance measurements
        2.2.2 Electrical stimulation
        2.2.3 Preparation of scanning electron microscope samples
        2.2.4 Immunofluorescence
        2.2.5 X-ray investigation technique
        2.2.6 Gross anatomy and histologic analyses
        2.2.7 Replication the bone healing process in vitro
        2.2.8 Creation of 3D Cell Culture S ystems
        2.2.9 Surgical procedure for creation rabbit bone CSD
        2.2.10 Statistical analysis
Chapter 3 The investigation of bone healing process with EIS using in vitromodel
    3.1 Introduction
    3.2 Investigation of in vitro bone healing process
        3.2.1 Investigation of the 3D models with different amount of collagen
        3.2.2 Investigation of the 3D models with different amount of HA
        3.2.3 Investigation of the 3D models with different amount of HAp
    3.3 Brief summary
Chapter 4 A dual-transduction-integrated biosensing system to examine the 3D cell culture for bone regeneration
    4.1 Introduction
    4.2 Investigation of bone-like 3D cell culture systems
        4.2.1 3D culture systems without cells and their characterization
        4.2.2 3D cell culture systems with living cells and their characterization
        4.2.3 Investigation of electrical stimulation of 3D cell culture system
    4.3 Brief summary
Chapter 5 The investigation of bone healing process with EIS using rabbitmodel
    5.1 Introduction
    5.2 Investigation in vivo bone healing process
        5.2.1 In vivo measurement of the electric impedance of bone healing
        5.2.2 Radiological findings
        5.2.3 Gross anatomy and histologic analyses
    5.3 Brief summary
Conclusions
References
Appendix
Papers published in the period of Ph.D. education
Acknowledgements
Resume



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