miR-338-3p靶向SIRT2调控Wnt/β-catenin信号通路影响胃癌细胞迁移和侵袭的机制研究
发布时间：2022-02-12 12:58
胃癌作为消化道常见的恶性肿瘤,多因胃上皮细胞的恶性转化而发生,是世界上第四大恶性肿瘤。2012年,全球新出现的胃癌患者在94万左右,其中至少有70万人因胃癌而死亡。胃癌的诱发因素十分复杂,幽门螺杆菌的定植,肥胖,特异性的饮食习惯等都可能是胃癌发生的原因,其早期症状不明显,大多数患者确诊时就处于肿瘤转移的中晚期治疗阶段,中位生存时间多在4-11个月,5年生存率则让人难以接受,还不足10%。虽然几年来早期诊断方法如内镜诊断和影像学诊断,以及治疗方法取得了许多革新,患者的整体存活率有所改善。但远处转移性胃癌患者的治疗仍面临巨大挑战,晚期胃癌患者的死亡多归因于腹膜种植,血行转移和淋巴结转移,因而,有必要探讨胃癌进展的作用机制,阐明胃癌细胞增殖、侵袭等生物学过程的发生原理。大量研究表明,非编码RNA对于胃癌的演变不可或缺。非编码RNA是不编码蛋白质,基于长度,非编码RNA被分成了不同的类别。大RNA包括长的非编码RNA（long non-coding RNA,lncRNA）,小核仁RNA,环状RNA,tRNA和rRNA,其长度都大于50核苷酸。小RNA包括微小RNA（microRNA,miRNA...
【文章来源】：重庆医科大学重庆市
【文章页数】：101 页
【学位级别】：博士
【部分图文】：
miR-338-3p在胃癌组织与邻近正常组织中的表达
重庆医科大学博士研究生学位论文28<0.05，图1.1）。此外，胃癌细胞系MGC-803，HGC-27，MGC-803，SGC-7901，BGC-823中miR-338-3p的表达水平显着低于GES-1胃粘膜上皮细胞（P<0.05，图1.2）。HGC-27细胞中miR-338-3p的表达水平最低，用于后续实验。图1.1miR-338-3p在胃癌组织与邻近正常组织中的表达。以qRT-PCR检测胃癌组织与邻近正常组织中miR-338-3p表达，以2-ΔΔCt法进行比较。*P＜0.05，与正常组比较。Fig.1.1ExpressionofmiR-338-3pingastriccancertissuesandadjacentnormaltissues.TheexpressionofmiR-338-3pingastriccancertissuesandadjacentnormaltissueswasdetectedbyqRT-PCRandcomparedby2-ΔΔCtmethod.*P<0.05,comparedwiththenormalgroup图1.2miR-338-3p在胃癌细胞系与胃粘膜上皮细胞中的表达。以qRT-PCR检测胃癌细胞MGC-803，HGC-27，MGC-803，SGC-7901，BGC-823与正常胃粘膜细胞GES-1中miR-338-3p表达差异，以2-ΔΔCt法进行比较。*P＜0.05，与GES-1比较。Fig.1.2ExpressionofmiR-338-3pingastriccancercelllinesandgastricmucosalepithelialcells.qRT-PCRwasusedtodetectthedifferenceofmiR-338-3pexpressionbetweengastriccancercellsMGC-803,HGC-27,MGC-803,SGC-7901,BGC-823andnormalgastricmucosalcellsGES-1,andcomparedby2-ΔΔCtmethod.*P<0.05,comparedtoGES-1.
重庆医科大学博士研究生学位论文292.2miR-338-3pmimics/inhibitor转染效率验证将miR-338-3pmimics/inhibitor转染到HGC-27细胞中，miR-338-3p的表达水平显着增加/减少，差异均有统计学意义（P<0.05，图1.3），mimics和inhibitor的相应对照的转染对于miR-338-3p表达均无明显影响（P>0.05，图1.3）。图1.3miR-338-3p转染效率验证。将miR-338-3pmimics/inhibitor转染到HGC-27细胞中，qRT-PCR检测miR-338-3p的表达水平改变，以2-ΔΔCt法进行比较。*P＜0.05，与miR-con组比较。Fig.1.3miR-338-3ptransfectionefficiencywasverified.miR-338-3pmimics/inhibitorwastransfectedintoHGC-27cells,andtheexpressionlevelofmiR-338-3pwasdetectedbyqRT-PCRandcomparedby2-ΔΔCtmethod.*P<0.05,comparedwiththemiR-congroup.2.3miR-338-3p上调对胃癌细胞增殖的影响MTT实验结果表明，与miR-con组相比，培养48h及72h时，miR-338-3p组的细胞增殖活性显着降低（P<0.05，图1.4）。
【参考文献】：
期刊论文
[1]MicroRNAs as non-invasive diagnostic biomarkers for gastric cancer:Current insights and future perspectives[J]. Alexander Link,Juozas Kupcinskas. World Journal of Gastroenterology. 2018(30)
[2]Three-microRNA signature identified by bioinformatics analysis predicts prognosis of gastric cancer patients[J]. Cheng Zhang,Chun-dong Zhang,Ming-hui Ma,Dong-qiu dai. World Journal of Gastroenterology. 2018(11)
[3]Yangzheng Sanjie decoction regulates proliferation and apoptosis of gastric cancer cells by enhancing let-7a expression[J]. Hai-Xia Deng,Ya-Ya Yu,Ao-Qiang Zhou,Jing-Li Zhu,Li-Na Luo,Wan-Qun Chen,Ling Hu,Geng-Xin Chen. World Journal of Gastroenterology. 2017(30)
[4]Micro RNA-145 exerts tumor-suppressive and chemoresistance lowering effects by targeting CD44 in gastric cancer[J]. Jian-Feng Zeng,Xiao-Qiu Ma,Lin-Pei Wang,Wei Wang. World Journal of Gastroenterology. 2017(13)
[5]Role of mi RNAs and their potential to be useful as diagnostic and prognostic biomarkers in gastric cancer[J]. Kelly Cristina da Silva Oliveira,Taíssa Maíra Thomaz Araújo,Camila Inagaki Albuquerque,Gabriela Alcantara Barata,Carolina Oliveira Gigek,Mariana Ferreira Leal,Fernanda Wisnieski,Fernando Augusto Rodrigues Mello Junior,André Salim Khayat,Paulo Pimentel de Assump??o,Rommel Mário Rodriguez Burbano,Marília Cardoso Smith,Danielle Queiroz Calcagno. World Journal of Gastroenterology. 2016(35)
[6]New blood markers detection technology: A leap in the diagnosis of gastric cancer[J]. Maneesh K Beeharry,Wen-tao Liu,Min Yan,Zheng-gang Zhu. World Journal of Gastroenterology. 2016(03)
[7]Emerging roles of non-coding RNAs in gastric cancer: Pathogenesis and clinical implications[J]. Shan-Shan Xie,Juan Jin,Xiao Xu,Wei Zhuo,Tian-Hua Zhou. World Journal of Gastroenterology. 2016(03)
[8]Recent advances in the molecular diagnostics of gastric cancer[J]. Mitsuro Kanda,Yasuhiro Kodera. World Journal of Gastroenterology. 2015(34)
[9]MiR-30b suppresses tumor migration and invasion by targeting EIF5A2 in gastric cancer[J]. Shu-Bo Tian,Jian-Chun Yu,Yu-Qin Liu,Wei-Ming Kang,Zhi-Qiang Ma,Xin Ye,Chao Yan. World Journal of Gastroenterology. 2015(31)
[10]Primary analysis and screening of microRNAs in gastric cancer side population cells[J]. Hai-Hong Zhang,Guo-Li Gu,Xiang-Yang Zhang,Feng-Zhi Li,Li Ding,Qin Fan,Ran Wu,Wei Shi,Xin-Yan Wang,Lin Chen,Xue-Ming Wei,Xiao-Ying Yuan. World Journal of Gastroenterology. 2015(12)
本文编号：3621763
【文章来源】：重庆医科大学重庆市
【文章页数】：101 页
【学位级别】：博士
【部分图文】：
miR-338-3p在胃癌组织与邻近正常组织中的表达
重庆医科大学博士研究生学位论文28<0.05，图1.1）。此外，胃癌细胞系MGC-803，HGC-27，MGC-803，SGC-7901，BGC-823中miR-338-3p的表达水平显着低于GES-1胃粘膜上皮细胞（P<0.05，图1.2）。HGC-27细胞中miR-338-3p的表达水平最低，用于后续实验。图1.1miR-338-3p在胃癌组织与邻近正常组织中的表达。以qRT-PCR检测胃癌组织与邻近正常组织中miR-338-3p表达，以2-ΔΔCt法进行比较。*P＜0.05，与正常组比较。Fig.1.1ExpressionofmiR-338-3pingastriccancertissuesandadjacentnormaltissues.TheexpressionofmiR-338-3pingastriccancertissuesandadjacentnormaltissueswasdetectedbyqRT-PCRandcomparedby2-ΔΔCtmethod.*P<0.05,comparedwiththenormalgroup图1.2miR-338-3p在胃癌细胞系与胃粘膜上皮细胞中的表达。以qRT-PCR检测胃癌细胞MGC-803，HGC-27，MGC-803，SGC-7901，BGC-823与正常胃粘膜细胞GES-1中miR-338-3p表达差异，以2-ΔΔCt法进行比较。*P＜0.05，与GES-1比较。Fig.1.2ExpressionofmiR-338-3pingastriccancercelllinesandgastricmucosalepithelialcells.qRT-PCRwasusedtodetectthedifferenceofmiR-338-3pexpressionbetweengastriccancercellsMGC-803,HGC-27,MGC-803,SGC-7901,BGC-823andnormalgastricmucosalcellsGES-1,andcomparedby2-ΔΔCtmethod.*P<0.05,comparedtoGES-1.
重庆医科大学博士研究生学位论文292.2miR-338-3pmimics/inhibitor转染效率验证将miR-338-3pmimics/inhibitor转染到HGC-27细胞中，miR-338-3p的表达水平显着增加/减少，差异均有统计学意义（P<0.05，图1.3），mimics和inhibitor的相应对照的转染对于miR-338-3p表达均无明显影响（P>0.05，图1.3）。图1.3miR-338-3p转染效率验证。将miR-338-3pmimics/inhibitor转染到HGC-27细胞中，qRT-PCR检测miR-338-3p的表达水平改变，以2-ΔΔCt法进行比较。*P＜0.05，与miR-con组比较。Fig.1.3miR-338-3ptransfectionefficiencywasverified.miR-338-3pmimics/inhibitorwastransfectedintoHGC-27cells,andtheexpressionlevelofmiR-338-3pwasdetectedbyqRT-PCRandcomparedby2-ΔΔCtmethod.*P<0.05,comparedwiththemiR-congroup.2.3miR-338-3p上调对胃癌细胞增殖的影响MTT实验结果表明，与miR-con组相比，培养48h及72h时，miR-338-3p组的细胞增殖活性显着降低（P<0.05，图1.4）。
【参考文献】：
期刊论文
[1]MicroRNAs as non-invasive diagnostic biomarkers for gastric cancer:Current insights and future perspectives[J]. Alexander Link,Juozas Kupcinskas. World Journal of Gastroenterology. 2018(30)
[2]Three-microRNA signature identified by bioinformatics analysis predicts prognosis of gastric cancer patients[J]. Cheng Zhang,Chun-dong Zhang,Ming-hui Ma,Dong-qiu dai. World Journal of Gastroenterology. 2018(11)
[3]Yangzheng Sanjie decoction regulates proliferation and apoptosis of gastric cancer cells by enhancing let-7a expression[J]. Hai-Xia Deng,Ya-Ya Yu,Ao-Qiang Zhou,Jing-Li Zhu,Li-Na Luo,Wan-Qun Chen,Ling Hu,Geng-Xin Chen. World Journal of Gastroenterology. 2017(30)
[4]Micro RNA-145 exerts tumor-suppressive and chemoresistance lowering effects by targeting CD44 in gastric cancer[J]. Jian-Feng Zeng,Xiao-Qiu Ma,Lin-Pei Wang,Wei Wang. World Journal of Gastroenterology. 2017(13)
[5]Role of mi RNAs and their potential to be useful as diagnostic and prognostic biomarkers in gastric cancer[J]. Kelly Cristina da Silva Oliveira,Taíssa Maíra Thomaz Araújo,Camila Inagaki Albuquerque,Gabriela Alcantara Barata,Carolina Oliveira Gigek,Mariana Ferreira Leal,Fernanda Wisnieski,Fernando Augusto Rodrigues Mello Junior,André Salim Khayat,Paulo Pimentel de Assump??o,Rommel Mário Rodriguez Burbano,Marília Cardoso Smith,Danielle Queiroz Calcagno. World Journal of Gastroenterology. 2016(35)
[6]New blood markers detection technology: A leap in the diagnosis of gastric cancer[J]. Maneesh K Beeharry,Wen-tao Liu,Min Yan,Zheng-gang Zhu. World Journal of Gastroenterology. 2016(03)
[7]Emerging roles of non-coding RNAs in gastric cancer: Pathogenesis and clinical implications[J]. Shan-Shan Xie,Juan Jin,Xiao Xu,Wei Zhuo,Tian-Hua Zhou. World Journal of Gastroenterology. 2016(03)
[8]Recent advances in the molecular diagnostics of gastric cancer[J]. Mitsuro Kanda,Yasuhiro Kodera. World Journal of Gastroenterology. 2015(34)
[9]MiR-30b suppresses tumor migration and invasion by targeting EIF5A2 in gastric cancer[J]. Shu-Bo Tian,Jian-Chun Yu,Yu-Qin Liu,Wei-Ming Kang,Zhi-Qiang Ma,Xin Ye,Chao Yan. World Journal of Gastroenterology. 2015(31)
[10]Primary analysis and screening of microRNAs in gastric cancer side population cells[J]. Hai-Hong Zhang,Guo-Li Gu,Xiang-Yang Zhang,Feng-Zhi Li,Li Ding,Qin Fan,Ran Wu,Wei Shi,Xin-Yan Wang,Lin Chen,Xue-Ming Wei,Xiao-Ying Yuan. World Journal of Gastroenterology. 2015(12)
本文编号：3621763
本文链接：https://www.wllwen.com/shoufeilunwen/yxlbs/3621763.html
