鳞头犬牙南极鱼卵壳蛋白的功能初步研究

发布时间：2018-04-10 19:28

  本文选题：卵壳蛋白 + CHO细胞　； 参考：《生物学杂志》2017年06期

【摘要】：已有研究表明卵壳蛋白ZPC5(Dissostichus mawsoni zona pellucida c5,Dm ZPC5)在南极鱼卵巢中大量表达,但是其具体功能尚不清楚。为了明确ZPC5是否参与鱼类抗寒过程,研究在中国仓鼠卵巢细胞(Chinese hamster ovary,CHO)中过表达南极鱼ZPC5,随之给予CHO细胞一定程度的低温处理,通过统计CHO细胞的存活率,来探讨zpc5在鱼类抗寒过程中的作用。存活率检测结果发现,过表达ZPC5的CHO细胞在0℃处理16 h后,其存活率较对照组显著上升(P0.001),证明zpc5在低温下可以促进细胞的存活。Western Blot检测结果显示,zpc5能在CHO细胞中高效表达,0℃处理后,CHO细胞内的ZPC5表达量显著上调,证明了低温可以诱导ZPC5的表达。研究结果表明,ZPC5有可能参与南极鱼鱼卵抗寒过程。
[Abstract]:It has been shown that ZPC5(Dissostichus mawsoni zona pellucida c5Dm ZPC5 is highly expressed in Antarctic fish ovary, but its specific function is still unclear.In order to determine whether ZPC5 is involved in the chilling resistance of fish, the expression of ZPC5 in Chinese hamster ovaries was studied in Chinese hamster ovary cells, and then CHO cells were treated with a certain degree of hypothermia, and the survival rate of CHO cells was calculated.To explore the role of zpc5 in the cold resistance of fish.The results of survival test showed that CHO cells expressing ZPC5 were treated at 0 鈩，

本文编号：1732583