蛋鸡肝脏功能和DNA甲基化与产蛋率的关系

发布时间：2018-04-13 10:14

本文选题：产蛋率 + 肝脏功能　；参考：《山东农业大学》2017年硕士论文

【摘要】：为研究产蛋后期产蛋率与肝脏功能及肝脏全基因组甲基化水平的关系,以海兰褐蛋鸡为试验材料,对不同周龄、产蛋率蛋鸡的肝功能指标和全基因组甲基化情况进行了观察分析。肝脏功能指标包括脂肪代谢、氧化应激、内质网应激、免疫应激这四个方面,肝脏全基因组甲基化情况主要从全基因组DNA甲基化图谱、全基因组甲基化水平、差异甲基化基因、GO注释、KEGG通路富集等方面进行了观察分析。试验一,不同周龄蛋鸡的肝脏功能研究。根据蛋鸡不同的周龄(25w和85w),选取16只高产蛋率的海兰褐蛋鸡分成两个组:25w高产蛋率组、85w高产蛋率组。结果显示,这2组蛋鸡在血清乳酸脱氢酶、碱性磷酸酶、谷草转氨酶、总蛋白、血清白蛋白、甘油三酯、总胆固醇、谷丙转氨酶水平上没有差异,85W蛋鸡血清葡萄糖水平显著升高(P0.05),SOD活性显著降低(P0.05)。试验二,不同产蛋率蛋鸡的肝脏功能研究。挑选85周龄蛋鸡不同的产蛋率(100%、70-80%、30-50%)共计24只海兰褐蛋鸡分成三个组:高产蛋率组、中等产蛋率组、低产蛋率组。结果表明,低产蛋率组蛋鸡血清碱性磷酸酶有下降趋势(P=0.0572)、葡萄糖水平有下降趋势(P=0.0832),中产蛋率组蛋鸡相比其他两组肝脏CAT活性显著降低(P0.05),蛋白质羰基活性显著升高(P0.05),ACC、FAS mRNA表达显著降低(P0.05),85W-middle组蛋鸡肝脏LXR mRNA表达显著升高(P0.05),SOD1 mRNA表达有降低趋势(P=0.0932),GPx1 mRNA表达有升高趋势(P=0.0911)。低产蛋率组蛋鸡肝脏甘油三酯含量显著升高(P0.05),TNF-αmRNA表达显著降低(P0.05)。试验三,不同产蛋率蛋鸡的肝脏甲基化研究。挑选70周龄蛋鸡不同产蛋率(100%、40-60%),16只海兰褐蛋鸡分成两个组:高产蛋率组、低产蛋率组。采用亚硫酸氢盐高通量测序技术,分析其全基因组甲基化。全基因组甲基化水平分别是56.98%、57.75%,不同染色体中C的甲基化水平、不同基因区域上各类型C甲基化水平、不同基因原件区域上各类型C甲基化水平结果中,低产蛋率组均高于高产蛋率组。由DMR(差异甲基化区域)得差异基因共3155,其中上调基因2172,下调基因983,低产蛋率组显著性上调基因远远多于下调基因。在upstream区域和CDS区域,功能富集分析得到显著富集GO分类注释18条(P0.05),得到显著富集pathway共三条(P0.05):嘌呤代谢、磷酸戊糖途径、甘油磷脂代谢。低产蛋率组(low laying rate)蛋鸡肝脏ATIC基因甲基化CDS区域上调,其表达水平显著升高(P0.05);低产蛋率组(low laying rate)蛋鸡肝脏ALDOB基因甲基化CDS区域上调,其表达水平显著升高(P0.05)。低产蛋率组蛋鸡肝脏LCPT1表达水平显著升高(P0.05),LXR表达水平显著升高(P0.05),ME1基因表达水平有降低趋势(P=0.0656)。综合以上结果,不同周龄产蛋率相近蛋鸡肝功能指标没有明显差别,同周龄不同产蛋率蛋鸡间肝功能及肝脏全基因组甲基化存在明显差异。
[Abstract]:In order to study the relationship between egg laying rate and liver function and the whole genome methylation level of liver, Hailan brown laying hens were used as experimental materials.The indexes of liver function and the whole genome methylation of laying hens were observed and analyzed.The liver function indexes include fat metabolism, oxidative stress, endoplasmic reticulum stress and immune stress. The whole genome methylation of liver is mainly based on the whole genome DNA methylation map and the whole genome methylation level.The enrichment of KEGG pathway of differential methylation gene (go) was observed and analyzed.Experiment 1: liver function of layers at different weeks of age.According to different weeks of age, 16 Hailan brown laying hens with high laying rate were divided into two groups: 1: 25 w high laying rate group and 85 w high laying rate group.The results showed that the two groups of layers in the serum lactate dehydrogenase, alkaline phosphatase, glutamic oxaloacetic transaminase, total protein, serum albumin, triglyceride, total cholesterol,There was no difference in the level of glutamate-pyruvate aminotransferase (alt). The serum glucose level of 85 W laying hens was significantly increased and the SOD activity of P0.05 was significantly decreased.The second experiment was to study the liver function of laying hens with different laying rates.A total of 24 Hailan brown layers were divided into three groups: high laying rate group, medium laying rate group and low laying rate group.The results show thatIn low laying rate group, the content of triglyceride in liver increased significantly, and the expression of TNF- 伪 mRNA decreased significantly.Experiment 3. Study on liver methylation of laying hens with different laying rates.Sixteen Hailan brown layers were divided into two groups: high laying rate group and low laying rate group.The whole genome methylation of H2SO4 was analyzed by high throughput sequencing technique.The whole genome methylation levels were 56.98 and 57.75, respectively. The levels of C methylation in different chromosomes, in different gene regions, in all types of C methylation levels in different gene regions, in different gene original regions, in all types of C methylation levels,The low laying rate group was higher than the high laying rate group.A total of 3155 differentially expressed genes were obtained from DMRs (differential methylation region). Among them, 2172 genes were up-regulated and 983down-regulated genes were down-regulated. In the low laying rate group, the up-regulated genes were much more than the down-regulated genes.In the upstream region and CDS region, the functional enrichment analysis showed that there were significant enrichment of P0.05 in 18 Go classification notes, and a significant enrichment of P0.05 in pathway: purine metabolism, pentose phosphate pathway, glycerol phospholipid metabolism.In low laying rate group, the methylation CDS region of ATIC gene in the liver of laying hens was upregulated, and its expression level was significantly increased (P 0.05), and the methylated CDS region of ALDOB gene in liver was up-regulated in low laying rate group (P 0.05).In low laying rate group, the expression level of LCPT1 in liver of laying hens increased significantly, and the expression level of LXR of P0.05An increased significantly. There was a tendency to decrease the expression level of P0.05AME1 gene in laying hens with a tendency of decreasing P0.0656.Based on the above results, there was no significant difference in the liver function index of the layers with the similar laying rate at different weeks, but there were significant differences in the liver function and the whole liver genome methylation among the layers with different laying rates at the same age.
【学位授予单位】：山东农业大学
【学位级别】：硕士
【学位授予年份】：2017
【分类号】：S831

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