基于SRAP和ISSR方法对东北地区紫丁香蘑遗传多样性的研究

发布时间：2018-04-19 16:04

  本文选题：香蘑属 + 种群　； 参考：《沈阳农业大学》2017年硕士论文

【摘要】：紫丁香蘑色泽宜人、菇香独特,在我国主要分布在北方地区。紫丁香蘑作为东北地区珍稀的野生食药用菌资源,由于其营养价值和药用价值不断被人们发现和认可,导致市场需求量不断增加,野生资源有限且人工栽培过程比较困难,这对紫丁香蘑野生资源的保护是极其不利的。利用分子标记的方法研究我国东北地区紫丁香蘑的遗传多样性,有助于了解它们的生存现状,在资源保护和栽培育种等方面都具有重要的意义。通过SRAP和ISSR分子标记方法对我国东北地区8个野生紫丁香蘑种群的72份样本材料进行研究分析。在SRAP分析中,从8个前端引物和8个后端引物组合成的64个引物组合中筛选出条带多态性好、重现性和清晰度高的6个SRAP引物组合,扩增紫丁香蘑的开放阅读框序列区域,总共获得117个条带,其中多态性条带有111条,多态性条带百分比为94.87%。在紫丁香蘑的物种水平上,Nei's基因多样性指数He = 0.3426,Shannon's遗传多样性信息指数I = 0.5049;种群间的Nei's平均遗传距离为0.2059,遗传分化系数Gst = 0.2136,基因流Nm= 1.8408,Nei's遗传距离和种群地理距离之间曼特尔相关性检验结果为r = 0.3377,P= 0.0580。在ISSR分析中,从23条ISSR引物中筛选出条带多态性好、重现性和清晰度高的6条ISSR引物扩增紫丁香蘑的重复序列,总共获得97个条带,其中多态性条带有91条,多态性条带百分比为93.81%。在紫丁香蘑的物种水平上,Nei's基因多样性指数He = 0.3393,Shannon's遗传多样性信息指数I=0.5033;种群间的Nei's平均遗传距离为0.2557,遗传分化系数Gst = 0.2666,基因流Nm = 1.3752,种群间的Nei's遗传距离和地理距离之间曼特尔相关性检验结果为r =0.1850,P = 0.1908。实验结果表明两种分子标记方法均适合用于分析紫丁香蘑的遗传多样性,都检测到紫丁香蘑具有较高的遗传多样性,其中SRAP标记多态性略高于ISSR标记。本研究结果表明紫丁香蘑不同种群间存在一定的遗传分化和基因流动,遗传分化主要发生在种群内,遗传距离和地理距离之间没有明显的相关性关系。
[Abstract]:Lilac mushroom color is pleasant, mushroom fragrance unique, mainly distributed in the north of China.As a rare resource of wild edible medicinal fungi in Northeast China, lilac mushroom has been found and recognized by people because of its nutritional value and medicinal value, which leads to increasing market demand, limited wild resources and difficult artificial cultivation process.This is extremely disadvantageous to the conservation of wild resources of lilac mushroom.Using molecular markers to study the genetic diversity of Tricholoma lilacs in Northeast China is helpful to understand their survival status and is of great significance in conservation of resources and cultivation and breeding.SRAP and ISSR molecular markers were used to study 72 samples of 8 wild populations of Tricholoma lilacs in Northeast China.In SRAP analysis, from 64 primer combinations composed of 8 front-end primer and 8 back-end primer, 6 SRAP primer combinations with high reproducibility and clarity were selected to amplify the open reading frame region of Tricholoma lanceolata.A total of 117 bands were obtained, of which 111 were polymorphic, the percentage of polymorphic bands was 94.87.The genetic diversity index he = 0.3426 Shannons genetic diversity information index I = 0.5049, the average Nei's genetic distance between populations is 0.2059, the genetic differentiation coefficient Gst = 0.2136, the gene flow Nm = 1.8408Neis genetic distance and population geography.The result of Mantel correlation test between distances was r = 0.3377 (P = 0.0580).In ISSR analysis, 6 ISSR primers with high reproducibility and clarity were selected from 23 ISSR primers to amplify the repeat sequence of Tricholoma lilygii. A total of 97 bands were obtained, among which 91 were polymorphic.The percentage of polymorphic bands was 93.81%.At the species level of Tricholoma lilygii, the genetic diversity index of Neis is he = 0.3393 and Shannons genetic diversity information index is 0.5033, the average genetic distance of Nei's among populations is 0.2557, the coefficient of genetic differentiation Gst = 0.2666, the gene flow Nm = 1.3752, the Nei's genetic distance between populations and the genetic distance between populations.The result of Mantel correlation test between geographical distance is r = 0.1850 P = 0.1908.The results showed that both of the two molecular markers were suitable for the analysis of genetic diversity of Tricholoma lilac, and both of them had higher genetic diversity, and the polymorphism of SRAP marker was slightly higher than that of ISSR marker.The results showed that there was a certain genetic differentiation and gene flow among different populations of Tricholoma lilacs. Genetic differentiation mainly occurred within the population, and there was no obvious correlation between genetic distance and geographical distance.
【学位授予单位】：沈阳农业大学
【学位级别】：硕士
【学位授予年份】：2017
【分类号】：S646
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本文编号：1773775