马铃薯StRac转基因植株生理指标以及广谱抗性的测定

发布时间：2018-05-29 12:02

本文选题：马铃薯 + StRac　；参考：《内蒙古农业大学》2017年硕士论文

【摘要】：小G蛋白是一类单体GTP结合蛋白,具有GTPase的活性。Rac是小G蛋白家族中的重要成员,已有研究结果表明小G蛋白Rac能够参与多种细胞信号调控过程,其中包括植物抗病防卫反应、花粉管的伸长、细胞分化、根毛的发育以及细胞骨架的重组等。本研究以.StRac转基因马铃薯植株为材料,测定StRac转基因植株的各项生理指标和广谱抗性水平,具体结果如下:1.马铃薯StRac转基因植株试管苗各项生理指标的测定结果表明,过量表达激活型CA-StRac基因植株的根/茎/叶的鲜重、干重、单株株高、茎粗、叶片数、叶面积、节长、节数、根系长度、根条数均比空载体P1300-GFP的高,分别增加了 33.33%、11.54%、34.44%、12.99%、25.50%、28.89%、22.83%、10%、2.30%、6.39%、46.10%、7.8%、25%、27.2%。相反,过量表达失活型DN-StRac基因植株的各项生理指标除茎粗比空载体P1300-GFP增加6.25%外,其余各项指标比空载体P1300-GFP有所降低,降低比率分别为 64.82%、42.31%、46.67%、63.28%、35.30%、40%、8.7%、8.4%、39%、4.7%、1.9%、35.42%、10.61%。2.马铃薯StRac转基因植株大田中各项生理指标测定的结果表明,过量表达激活型CA-StRac基因植株的花期比空载体P1300-GFP仅仅提早一天。单个薯块的大小、重量、芽眼数、分枝数分别比空载体P1300-GFP增加了 22.12%、49.82%、2.71%、10.41%。3.马铃薯StRac转基因植株广谱抗性鉴定结果表明,接种早疫病菌、黑痣病菌、枯萎病菌和黄萎病菌后,过量表达CA-StRac的转基因马铃薯抗性均有所提高;其中接种早疫病菌后转基因马铃薯叶片上病斑面积相比对照降低了 72.8%;在薯片上接种黑痣病菌后,病斑面积也相应降低了 35.6%;接种枯萎病菌和黄萎病菌后,病情指数相比对照也相应降低了 40%和11.6%。说明StRac能够正向调控马铃薯广谱抗性的建立。
[Abstract]:Small G protein is a kind of monomeric GTP binding protein, which has the activity of GTPase. Racc is an important member of small G protein family. It has been shown that small G protein Rac can participate in many cell signal regulation processes, including plant disease resistance and defense response. Pollen tube elongation, cell differentiation, root hair development and cytoskeleton recombination. In this study, the physiological indexes and broad-spectrum resistance of StRac transgenic potato plants were determined. The results were as follows: 1. The physiological indexes of potato StRac transgenic plantlets in vitro were determined. The results showed that the fresh weight, dry weight, plant height, stem diameter, leaf number, leaf area, node length and node number of over-expressed CA-StRac gene plants were measured. The length of root system and the number of root strips were higher than that of empty carrier P1300-GFP, and they were increased by 33.333.11.54 and 12.99, and 25.50 and 28.89, respectively, and there was a score of 22.83and a score of 22.833.It was about 2.306.396.390.46.100.7.8250.27.20.The root length and the number of roots were higher than those of the empty carrier P1300-GFP, and the number of roots was increased by 33.333.11.54 and 11.54, respectively. On the contrary, with the exception of 6.25% increase in physiological index of inactivated DN-StRac gene plant, the other physiological indexes of inactivated DN-StRac gene plant were lower than that of empty vector P1300-GFP by 6.25%, and the other indexes were decreased by 64.82% (64.82%) than empty vector P1300-GFP (64.82%) by the ratio of 63.2835.308.78.78.43.4394.77.The other indexes were lower than that of empty vector P1300-GFP (64.82%). The physiological indexes of potato StRac transgenic plants were determined in the field. The results showed that the flowering time of the plants with overexpression of activated CA-StRac gene was only one day earlier than that of empty vector P1300-GFP. Compared with empty carrier P1300-GFP, the size, weight, bud eye number and branching number of a single tuber increased 22.12% and 10.41% than that of empty carrier P1300-GFP. The results of broad-spectrum resistance identification of potato StRac transgenic plants showed that after inoculation with early blight, nevus, Fusarium wilt and Verticillium wilt, the resistance of transgenic potato with overexpression of CA-StRac was increased. After inoculation with Phytophthora infestans, the leaf spot area of transgenic potato decreased by 72.8 compared with the control. After inoculating potato chips with nevus, the spot area was also reduced by 35.60.After inoculation with Fusarium wilt and Verticillium wilt, The disease index was also decreased by 40% and 11. 6% compared with the control. The results showed that StRac could positively regulate the establishment of broad-spectrum resistance in potato.
【学位授予单位】：内蒙古农业大学
【学位级别】：硕士
【学位授予年份】：2017
【分类号】：S532

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