种猪场猪伪狂犬病的净化技术

发布时间：2018-05-29 15:19

本文选题：伪狂犬病毒 + 疫病控制　；参考：《河南科技大学》2017年硕士论文

【摘要】：近年来,我国养猪业发展迅猛,规模化、集团化、产业化养猪企业不断涌出,养猪业占据畜牧业,乃至农业重要地位。但是,养猪疫病防控技术薄弱,特别是以猪伪狂犬病为主的病毒性疫病依然严重,威胁着养猪业健康和可持续发展,必须研究和探索出一套解决猪场伪狂犬病综合防控技术。猪伪狂犬病(Pseudorabies,PR)是由伪狂犬病病毒(Pseudorabies virus.PRV)引起的病毒性传染病,发病率和死亡率高,危害性大,呈世界性分布,严重影响着养猪业健康和持续性发展,对种猪企业更是致命性伤害。实验目的:本实验通过现场管理和实验室技术手段等方法,发现、分离和清除种猪场伪狂犬野毒阳性猪群,逐步实现伪狂犬病的净化和根除,寻找出一套解决种猪场疫病综合防控的技术路线。实验方案:实验在河南省某省直科研单位下属原种猪场,并根据疫病防控技术要求对猪场进行改造升级,技术依托河南省农业科学院动物免疫实验室。实验分3个阶段完成,第一阶段为检测分离,采取对种猪群全部,保育猪和肥育猪部分进行定性定量检测,选择IDEXX猪伪狂犬gE抗体检测试剂盒及gB抗体检测试剂盒作为猪伪狂犬病原抗体及疫苗抗体的检测手段;选择荧光定量PCR诊断作为ELISA抗体检测的定性补充,阳性或可疑个体猪群离群淘汰;第二阶段为调整提高,根据检测结果调整免疫方案,改善猪场硬件条件,调整营养供给、强化生物安全实施,精细化饲养管理和程序化疫病防控技术等综合措施,致使猪群由阳性转阴;第三阶段为维持稳定,定期检测猪群,阳性淘汰,保持阴性。实验结果:本实验经过3年综合防治措施完成后,伪狂犬病得以控制、稳定和净化,伪狂犬阳性率从10.87%逐步下降到1.92%和0.03%,抗体水平的整齐度提高,免疫合格率显著提升;各项生产指标较实验实施前有较大的提高和改善,成功解决种猪场伪狂犬病净化难题,并形成了一套疫病防控技术体系集成,为河南省乃至全国种猪企业疫病综合防治提供了理论依据、实践探索和技术支撑。
[Abstract]:In recent years, the pig industry in our country has developed rapidly, large-scale, collectivized and industrialized pig raising enterprises are constantly pouring out. Pig industry occupies an important position in animal husbandry and even agriculture. However, the technology of prevention and control of pig epidemic disease is weak, especially the virus disease which is mainly pseudorabies is still serious, which threatens the healthy and sustainable development of pig industry. It is necessary to study and explore a set of comprehensive prevention and control technology to solve pseudorabies in pig farm. Pseudorabies virus (PRV) is a viral infectious disease caused by pseudorabies virus (PRV). It has a high incidence and mortality rate, and has a worldwide distribution, which seriously affects the health and sustainable development of pig industry, and is even more fatal to pig breeding enterprises. Objective: by means of field management and laboratory techniques, we found out, isolated and eliminated pseudorabies virus positive pigs in pig farm, and gradually realized the purification and eradication of pseudorabies. To find out a set of comprehensive prevention and control of pig plant disease technical route. Experimental scheme: the experiment was carried out in the original pig farm of a provincial scientific research unit in Henan Province, and the pig farm was upgraded according to the technical requirements of epidemic disease prevention and control. The technology relies on the Animal Immunization Laboratory of Henan Academy of Agricultural Sciences. The experiment was completed in three stages. In the first stage, qualitative and quantitative tests were carried out on all breeds, conservation pigs and fattening pigs in the first stage. IDEXX porcine pseudorabies GE antibody detection kit and GB antibody detection kit were selected as the detection methods of porcine pseudorabies antibody and vaccine antibody, and fluorescence quantitative PCR diagnosis was selected as the qualitative supplement for ELISA antibody detection. The second stage is to adjust and improve the immunization program according to the test results, improve the hardware condition of pig farm, adjust nutrition supply, and strengthen the implementation of biosafety. The comprehensive measures such as fine feeding management and programmed epidemic disease prevention and control technology resulted in the positive turn to negative in the pig population; the third stage was to maintain stability and test the pig herd regularly and to eliminate the positive and keep negative in the third stage. Results: after 3 years of comprehensive prevention and treatment, pseudorabies was controlled, stabilized and purified. The positive rate of pseudorabies decreased gradually from 10.87% to 1.92% and 0.03%. The production indexes were greatly improved and improved compared with those before the experiment, which successfully solved the problem of pseudorabies purification in pig farms, and formed a set of integrated technology system for the prevention and control of epidemic diseases. It provides theoretical basis, practical exploration and technical support for comprehensive prevention and control of epidemic disease in Henan province and even the whole country.
【学位授予单位】：河南科技大学
【学位级别】：硕士
【学位授予年份】：2017
【分类号】：S858.28

【参考文献】