基于SCoT标记的内蒙古地区西伯利亚杏遗传多样性

发布时间：2018-05-30 19:08

本文选题：西伯利亚杏 + 种源　；参考：《内蒙古农业大学》2017年硕士论文

【摘要】：本研究基于SCoT分子标记利用23个引物对内蒙古西伯利亚杏10个种源的278个家系进行遗传多样性,研究探讨内蒙古地区山杏资源的收集及保护,为内蒙古西伯利亚杏的保存与利用提供有效依据。结果显示:1.23个引物共扩增出292个位点,其中多态性位点(NPB)为289个,平均每个引物扩增的多态性位点为12.6条,SCoT21、SCoT32、SCoT53三个引物扩增的位点最多为17条,多态性比率为100%;引物SCoT3扩增的位点最少为9条,多态性比率为90%。2.内蒙古西伯利亚杏种源引物多态性位点比率为97.94%,等位基因数(Na)为1.9803,有效等位基因数(Ne)为1.4252,Nei's遗传多样性指数(H)为0.2571,Shannon's信息多样性指数(I)为0.3989;10个种源等位基因数范围在1.3934～1.6230;有效等位基因数范围在1.1956～1.3130;Nei's遗传多样性指数范围0.1181～0.1871;Shannon's信息多样性指数范围在0.1812～0.2859;其中科右中旗种源的遗传多样性水平最高,其次是科左后旗;万家沟种源的遗传多样性最低。遗传分化系数(Gst)为0.3601,基因流(Nm)为0.8884。经分子方差分析39%的遗传变异来自种源间,61%的遗传变异来自种源内。3.内蒙古西伯利亚杏种源遗传相似度范围0.7799～0.9694,遗传距离范围在0.0311～0.2486:基于遗传相似度的聚类分和遗传距离的主坐标分析均将供试10个种源分成2组,大部分地理距离相近的种源聚类到一起。研究表明,内蒙古西伯利亚杏有较高的遗传多样性。
[Abstract]:In this study, the genetic diversity of 278 families from 10 provenances of Siberian apricot in Inner Mongolia was studied by using 23 primers based on SCoT molecular markers, and the collection and protection of apricot resources in Inner Mongolia were studied. It provides an effective basis for the preservation and utilization of Siberian apricot in Inner Mongolia. The results showed that 292 loci were amplified by 23 primers, of which 289 were polymorphic loci (NPB), and the average number of polymorphic loci per primer amplified was 12.6 SCoT21 / SCoT32SCoT53. The polymorphic ratio was 100 and the primer SCoT3 amplified the least 9 loci and the polymorphic ratio was 90. 2. 2. In Inner Mongolia, the ratio of primer polymorphic loci was 97.94, the number of alleles was 1.9803, the effective number of alleles was 1.4252Neis genetic diversity index (HH) was 0.2571Shannon 's information diversity index (I) was 0.3989.The allele number of 10 provenances was in the range of 1.9803, and the number of alleles in 10 provenances was in the range of 1.9803. The number of effective alleles was in the range of 1.19561.3130Neis genetic diversity index 0.1181 / 0.1871 Shannons information diversity index was 0.18120.2859.The genetic diversity level of the middle flag provenance was the highest. The genetic diversity of Wanjiagou provenance was the lowest. The coefficient of genetic differentiation was 0.3601, and the coefficient of genetic differentiation was 0.8884. By molecular variance analysis, 39% of the genetic variation came from the interprovenance. 61% of the genetic variation came from within the provenance. In Inner Mongolia, the genetic similarity of Siberian apricot provenances was 0.7799 / 0.9694, and the genetic distance was 0.03110.2486. The cluster and the principal coordinate analysis of genetic distance based on genetic similarity divided 10 provenances into two groups. Most provenances with similar geographical distances are clustered together. The results showed that there was high genetic diversity in Siberian apricot in Inner Mongolia.
【学位授予单位】：内蒙古农业大学
【学位级别】：硕士
【学位授予年份】：2017
【分类号】：S662.2

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