外源激素对草莓果实早期发育及FvCO4基因表达的影响
发布时间:2018-07-10 05:31
本文选题:草莓 + 幼果发育 ; 参考:《沈阳农业大学》2017年硕士论文
【摘要】:果实的形成和发育是包括草莓在内的瓜果类园艺作物生产的基础。其中植物激素对果实发育和成熟的调控是果树学科研究的重要问题,了解激素与果实发育的关系可为果实品质改良、优良品种培养等提供理论参考,也可生为产上应用植物激素来改善果实的品质和增加果实的产量提供依据。本研究以未授粉材料森林草莓YW5AF7为试材,喷施不同激素于发育早期的幼果,调查其对草莓果实早期发育的影响,同时检测疑似相关基因FvCO4在草莓果实髓部和皮层中的表达变化,为深入了解和阐述外源激素对草莓果实早期发育的影响提供试验依据。主要的研究结果如下:1.研究了不同浓度的GA_3对森林草莓YW5AF7幼果早期生长发育的影响。试验发现采用的不同浓度(400μmol/L、500μmol/L、600μmol/L)GA_3对草莓幼果早期发育均有促进作用,可促进草莓果实髓部和皮层的生长、膨大发育。其中浓度为500 μmol/L的GA_3作用效果最为显著。2.分析对比了不同激素(组合)对森林草莓YW5AF7幼果早期生长发育的影响。结果表明,相同浓度(500μmol/L)的不同激素处理(GA_3、NAA、NAA+GA_3)均能促进草莓幼果早期发育,其中以500 μmol/L浓度的NAA+GA_3处理影响最为明显,该处理下幼果发育大小明显比同时期其他两种激素处理下的幼果发育更大。3.通过定量RT-PCR的方法检测了外源激素对草莓果实中FvCO4基因表达变化的影响。检测发现,与对照相比,三种激素处理(GA_3、NAA、NAA+GA_3)均可明显上调该基因的表达,其中在幼果形成前期FvCO4的表达上调最为明显。
[Abstract]:Fruit formation and development are the basis for the production of melon and fruit horticultural crops, including strawberries. The regulation of plant hormones on fruit development and maturation is an important issue in fruit tree research. Understanding the relationship between hormone and fruit development can provide theoretical reference for fruit quality improvement and cultivation of fine varieties. It can also provide the basis for the application of plant hormones to improve the quality of fruit and increase the yield of fruit. In this study, unpollinated forest strawberry YW5AF7 was sprayed with different hormones to investigate the effect of YW5AF7 on the early development of strawberry fruit, and the expression of FvCO4 gene was detected in the pulp and cortex of strawberry fruit. To provide experimental basis for further understanding and expounding the effect of exogenous hormones on the early development of strawberry fruit. The main findings are as follows: 1: 1. The effects of different concentrations of GA3 on the early growth and development of young fruits of forest strawberry YW5AF7 were studied. It was found that different concentrations of GA3 (400 渭 mol / L, 500 渭 mol / L, 600 渭 mol / L) could promote the early development of strawberry young fruit, promote the growth of pulp and cortex, and expand development of strawberry fruit. The effect of GA3 at 500 渭 mol / L was the most significant. The effects of different hormones (combinations) on the early growth and development of forest strawberry YW5AF7 young fruit were analyzed and compared. The results showed that different hormone treatments with the same concentration (500 渭 mol / L) could promote the early development of strawberry young fruit, especially 500 渭 mol / L NAA / L NAAGA3 treatment. The developmental size of young fruit under this treatment was significantly larger than that of the other two hormone treatments in the same period. The effects of exogenous hormones on the expression of FvCO4 gene in strawberry fruit were detected by quantitative RT-PCR. The results showed that compared with the control, the expression of FvCO4 was significantly up-regulated by the three hormone treatments (GA3s / NAANAA / GA3), especially in the early stage of young fruit formation.
【学位授予单位】:沈阳农业大学
【学位级别】:硕士
【学位授予年份】:2017
【分类号】:S668.4
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