苏尼特羊BMPR-IB、BMP15和GDF9基因与多胎性状关联性分析及BMP15基因突变检测

发布时间：2018-07-17 07:32

【摘要】：苏尼特羊属蒙古羊品种,具有耐寒、易放牧和肉质鲜美等优良特性,其不足之处是生产性能较低。本研究以300只苏尼特羊(其中产单羔188只,产双羔112只)为研究对象,同时选取小尾寒羊和湖羊各50只作为对照,整个研究群体总数为400只。利用聚合酶链反应限制性片段长度多态性PCR-RFLP结合DNA测序技术,分析BMPR-IB、BMP15和GDF9三个基因中多个不同突变位点对苏尼特羊多胎性状的影响,旨在为提高苏尼特羊的繁殖力提供理论支持。主要研究结果有:(1)在部分产双羔的苏尼特羊群中发现FecB突变(A746G),突变类型为杂合型(AG)和纯合突变型(GG),基因型频率分别为0.089和0.009,A和G等位基因频率为0.946和0.054;产双羔苏尼特羊群体在该位点PIC=0.097,表现为低度多态;产单羔苏尼特羊群体在该位点无多态性;产单羔苏尼特羊群体中G等位基因频率显著低于产双羔苏尼特羊群体中G等位基因频率(P0.01);关联性分析表明,FecB位点与苏尼特羊的双羔率有关(P0.001)。(2)所选苏尼特羊群体在BMP15基因的FecXB、FecXL、FecXH、FecXG和FecXI五个位点中均未发现突变,全部为纯合野生型。(3)所选苏尼特羊群体在GDF9基因上的FecGH FecGF、FecGE和G4四个位点上均无多态性,全部为纯合野生型。(4)通过测序分析首次发现部分苏尼特羊BMP15基因的内含子发生以下突变:A115G、G117A、C342A、G419A、A1168G 以及 G2129T。本研究结果证明,FecB突变位点可作为分子标记用于提高苏尼特羊产羔率,然而苏尼特羊双羔性状的分子遗传机制仍有待进一步深入研究。
[Abstract]:Sunita sheep is a Mongolian sheep breed, which has the advantages of cold tolerance, easy grazing and delicious meat quality, but its production performance is low. In this study, 300 Sunita sheep (including 188 single lambs and 112 double lambs) were selected as control, and 50 small tail Han sheep and 50 Hu sheep were selected as control, the total number of the whole research population was 400. Polymerase chain reaction restriction fragment length polymorphism (PCR-RFLP) and DNA sequencing technique were used to analyze the effects of different mutation sites of BMPR-IBBMP15 and GDF9 genes on the fertility traits of Sunita sheep. The aim is to provide theoretical support for improving the fecundity of Sunita sheep. The main results are as follows: (1) FecB mutation (A746G) was found in some Sunita sheep with two lambs, the mutation types were heterozygous (AG) and homozygous (GG), the genotype frequencies were 0.089 and 0.009A and G allele frequencies were 0.946 and 0.054, respectively; The population of Niett sheep showed low polymorphism at PIC-0.097 at this locus. There was no polymorphism at this locus in single lamb Sunita sheep population. The frequency of G allele in single lamb Sunita sheep was significantly lower than that in double lamb Sunita sheep (P0.01), and the correlation analysis showed that the frequency of G allele in Sunita sheep was related to the rate of double lambs (P0. 001). (2). No mutations were found at the five FecXHG and FecXI loci of the BMP15 gene. All were homozygous wild type. (3) there was no polymorphism at the four loci of FecGH FecGFGE and G4 on the GDF9 gene in the selected Sunita sheep population. All of them were homozygous wild type. (4) the following mutations were found in the intron of part of Sunita sheep BMP15 gene by sequencing. The following mutations occurred in the intron of the BMP15 gene: 10 A115G, G117A, C342A, G419A, A1168G and G2129T. The results show that the FecB mutation site can be used as a molecular marker to improve the lambing rate of Sunita sheep. However, the molecular genetic mechanism of double lamb traits of Sunita sheep needs to be further studied.
【学位授予单位】：内蒙古大学
【学位级别】：硕士
【学位授予年份】：2017
【分类号】：S826

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