沙葱种子对贮藏陈化应答机制的研究
发布时间：2018-07-28 12:17
【摘要】：沙葱(Allium mongolicum Regel.),地方名为蒙古韭,是生长在内蒙古、甘肃、新疆等荒漠地带、沙漠周边和山石缝隙等干旱地的一种野生蔬菜。沙葱作为一种潜在价值极高的野生蔬菜受到人民群众的热捧。为此笔者以贮藏1～10年沙葱种子为研究对象,考查其不同贮藏年限条件下干种子、萌发3天和10天种子的萌发特性、生理关键酶活性、呼吸作用等方面,并通过高通量测序技术对贮藏2015年(S1)、2012年(S2)、2008年(S4)这三个年份的沙葱种子萌发的转录组进行测序,并对其进行基因注释和功能分类,对其S1、S2、S4的差异基因进行了分析,为沙葱种子在不同萌发条件下的活力及寿命的生理生化响应机制和沙葱分子研究提供一定的理论依据。获得研究结果如下:1.随着沙葱种子贮藏陈化时间的延长,其发芽率、发芽势、发芽指数和活力指数均呈现先升高后缓慢降低的趋势,在贮藏3年的种子活力最高,发芽率高达89.00%;含水量呈现逐渐降低的变化趋势,电导率呈现逐渐升高的变化趋势。2.随着沙葱种子贮藏陈化时间的延长,呼吸速率与细胞色素C氧化酶均呈先升高后降低的变化趋势;沙葱种子在萌发过程中的关键酶活性变化是不同的,其中酸性磷酸酶和苹果酸脱氢酶活性均随陈化时间延长呈现先升高后降低的变化趋势,且贮藏5年酶活力最高;异柠檬酸脱氢酶活性变化规律不显著,而过氧化物酶活性呈现先降低后升高再降低的变化趋势。3.对不同贮藏年限的沙葱种子(S1、S2、S4)萌发构建了 RNA-seq进行转录组测序文库,经Illumina测序和组装得到了 79,703条Unigene序列,其平均长度为684bp。其中有有36,232条分别注释到NR、Swiss-Prot、KEGG、COG数据库中得到了基因功能注释信息,为深入研究不同贮藏年下下沙葱种子的相关基因提供了理论依据,也为沙葱种子生物信息学提供了数据资源。4.沙葱种子萌发的基因表达谱研究,3个不同贮藏时期测序结果两两比较,分别得到差异表达基因S1-10-VS-S2-10 5,723条,有75%上调;S1-10-VS-S4-10中7,347 条,有 59%上调;S2-10-VS-S4-10 中 5,495 条,有 48%上调。通过 GO Pathway分析发现,S1-10-VS-S2-10主要受影响的生物功能受影响最大的是代谢过程;受影响最小的是生物功能过程的蛋白结合的转录因子;S1-10-VS-S4-10和S2-10-VS-S4-10参与调控最多的是生物功能。通过差异表达基因在KEGG代谢通路中的功能富集分析,发现S1、S2、S4三个不同贮藏年份差异表达基因参与的代谢通路差异很大,S1对应到个类通路的Unigene数量高于其他两个年限,与总差异基因表达量趋势基本一致。
[Abstract]:Allium mongolica (Allium mongolicum Regel.), is a wild vegetable growing in the desert areas of Inner Mongolia Gansu Xinjiang and other arid areas such as the desert and the gap between mountains and rocks. As a kind of wild vegetable with high potential value, Allium balsicum is popular among the people. For this reason, the seeds of Allium mongolica were stored for 1 ~ 10 years. The germination characteristics, physiological key enzyme activities and respiration of dry seeds, germination days and 10 days of germination were investigated under different storage years. The transcriptome of seed germination of Allium mongolicum in 2015 (S1), 2012 (S2) and 2008 (S4) was sequenced by high-throughput sequencing technique, and its gene annotation and functional classification were carried out, and the differential genes of S1-S2OS4 were analyzed. It provides a theoretical basis for the physiological and biochemical response mechanism of seed vigor and longevity of Allium mongolicum under different germination conditions and the molecular studies of Allium mongolicum. The results are as follows: 1. The germination rate, germination potential, germination index and vigor index of the seeds increased first and then decreased slowly with the prolonging of storage time, and the seed vigor was the highest after 3 years of storage. The germination rate was up to 89.00, the water content decreased gradually, and the electrical conductivity increased gradually. The respiration rate and cytochrome C oxidase increased firstly and then decreased with the prolongation of storage and aging time of Allium mongolica seeds, and the changes of key enzyme activities were different during germination. The activity of acid phosphatase and malate dehydrogenase increased first and then decreased with aging time, and the activity of isocitrate dehydrogenase was the highest in 5 years, but the change of isocitrate dehydrogenase activity was not significant. The activity of peroxidase decreased first and then increased and then decreased. The RNA-seq transcriptome sequencing library was constructed for the germination of the seeds of Allium mongolicum (S1, S2, S4) for different storage years. 79703 Unigene sequences were obtained by Illumina sequencing and assembly, with an average length of 684 BP. 36232 of them were annotated in the database of NRRS-Swiss-ProtKEGGG, which provided theoretical basis for the further study of the genes related to the seeds of Allium mongolicum under different storage years, and provided a data resource of .4for the bioinformatics of the seeds of Allium mongolica. The gene expression profile of seed germination of Allium mongolicum L. was studied. The sequencing results of three different storage periods showed that there were 5723 differentially expressed genes (S1-10-VS-S2-10), 7347 of S1-10-VS-S4-10, 5495 of S2-10-VS-S4-10, and 48% of S2-10-VS-S4-10, respectively, with 75% upregulation of S1-10-VS-S4-10 and 59% of up-regulation of S2-10-VS-S4-10, respectively. Go Pathway analysis showed that the major affected biological function of S1-10-VS-S2-10 was the metabolic process, and the transcriptional factor S1-10-VS-S4-10 and S2-10-VS-S4-10 were the most involved in the regulation of biological function. Based on the functional enrichment analysis of differentially expressed genes in the KEGG metabolic pathway, it was found that the number of Unigene in the three differentially expressed genes involved in the three different storage years was higher than that in the other two years. The trend of total differentially expressed genes was basically consistent with that of total differentially expressed genes.
【学位授予单位】：内蒙古农业大学
【学位级别】：硕士
【学位授予年份】：2017
【分类号】：S647
本文编号：2150116
[Abstract]:Allium mongolica (Allium mongolicum Regel.), is a wild vegetable growing in the desert areas of Inner Mongolia Gansu Xinjiang and other arid areas such as the desert and the gap between mountains and rocks. As a kind of wild vegetable with high potential value, Allium balsicum is popular among the people. For this reason, the seeds of Allium mongolica were stored for 1 ~ 10 years. The germination characteristics, physiological key enzyme activities and respiration of dry seeds, germination days and 10 days of germination were investigated under different storage years. The transcriptome of seed germination of Allium mongolicum in 2015 (S1), 2012 (S2) and 2008 (S4) was sequenced by high-throughput sequencing technique, and its gene annotation and functional classification were carried out, and the differential genes of S1-S2OS4 were analyzed. It provides a theoretical basis for the physiological and biochemical response mechanism of seed vigor and longevity of Allium mongolicum under different germination conditions and the molecular studies of Allium mongolicum. The results are as follows: 1. The germination rate, germination potential, germination index and vigor index of the seeds increased first and then decreased slowly with the prolonging of storage time, and the seed vigor was the highest after 3 years of storage. The germination rate was up to 89.00, the water content decreased gradually, and the electrical conductivity increased gradually. The respiration rate and cytochrome C oxidase increased firstly and then decreased with the prolongation of storage and aging time of Allium mongolica seeds, and the changes of key enzyme activities were different during germination. The activity of acid phosphatase and malate dehydrogenase increased first and then decreased with aging time, and the activity of isocitrate dehydrogenase was the highest in 5 years, but the change of isocitrate dehydrogenase activity was not significant. The activity of peroxidase decreased first and then increased and then decreased. The RNA-seq transcriptome sequencing library was constructed for the germination of the seeds of Allium mongolicum (S1, S2, S4) for different storage years. 79703 Unigene sequences were obtained by Illumina sequencing and assembly, with an average length of 684 BP. 36232 of them were annotated in the database of NRRS-Swiss-ProtKEGGG, which provided theoretical basis for the further study of the genes related to the seeds of Allium mongolicum under different storage years, and provided a data resource of .4for the bioinformatics of the seeds of Allium mongolica. The gene expression profile of seed germination of Allium mongolicum L. was studied. The sequencing results of three different storage periods showed that there were 5723 differentially expressed genes (S1-10-VS-S2-10), 7347 of S1-10-VS-S4-10, 5495 of S2-10-VS-S4-10, and 48% of S2-10-VS-S4-10, respectively, with 75% upregulation of S1-10-VS-S4-10 and 59% of up-regulation of S2-10-VS-S4-10, respectively. Go Pathway analysis showed that the major affected biological function of S1-10-VS-S2-10 was the metabolic process, and the transcriptional factor S1-10-VS-S4-10 and S2-10-VS-S4-10 were the most involved in the regulation of biological function. Based on the functional enrichment analysis of differentially expressed genes in the KEGG metabolic pathway, it was found that the number of Unigene in the three differentially expressed genes involved in the three different storage years was higher than that in the other two years. The trend of total differentially expressed genes was basically consistent with that of total differentially expressed genes.
【学位授予单位】：内蒙古农业大学
【学位级别】：硕士
【学位授予年份】：2017
【分类号】：S647
【参考文献】
相关期刊论文 前10条
1 姜兴睿;;种子加工及贮藏方法研究探析[J];种子科技;2016年06期
2 于竞;;对影响种子贮藏因素的分析[J];种子世界;2016年05期
3 刘娟;归静;高伟;马俊峰;王Oz珍;;种子老化的生理生化与分子机理研究进展[J];生态学报;2016年16期
4 严子柱;满多清;李得禄;;沙葱(Allium mongolicum)解剖结构与抗旱性[J];中国沙漠;2015年04期
5 李满堂;张仕林;邓鹏;侯喜林;王建军;;洋葱转录组SSR信息分析及其多态性研究[J];园艺学报;2015年06期
6 廉洁;张喜春;谷建田;;转录组学及其在蔬菜学上应用研究进展[J];中国农学通报;2015年08期
7 苏仲冰;;种子贮藏方法[J];农民致富之友;2015年03期
8 闫慧芳;夏方山;毛培胜;;种子老化及活力修复研究进展[J];中国农学通报;2014年03期
9 刘海琳;;农作物种子贮藏方法[J];农民致富之友;2014年01期
10 马晓伟;田秀英;王定元;徐艳华;巴图巴根;刘佳峗;;野生沙葱人工驯化采种栽培技术[J];蔬菜;2013年12期
相关博士学位论文 前5条
1 杜方;百合不同器官转录组分析及SSR标记开发应用[D];浙江大学;2014年
2 孙秀东;大蒜转录组测序及核因子YB3的功能研究[D];山东农业大学;2013年
3 王晓娟;沙葱胚胎学及种子休眠机理的研究[D];内蒙古农业大学;2011年
4 张巧娥;沙葱提取物的分离鉴定及其对绵羊消化道共轭亚油酸含量和胴体脂肪沉积影响的研究[D];内蒙古农业大学;2007年
5 赵国芬;沙葱和油料籽实对绵羊瘤胃发酵、营养物质消化流通及胴体品质影响的研究[D];内蒙古农业大学;2005年
相关硕士学位论文 前4条
1 于倩;基于转录组对黄芪种子萌发过程及其内源激素分析[D];内蒙古大学;2016年
2 严子柱;沙葱生态生理特性及驯化栽培技术研究[D];甘肃农业大学;2007年
3 杨忠仁;沙葱种子生物学特性研究[D];内蒙古农业大学;2006年
4 李佳;新疆部分葱属植物生物学特性及其分类意义研究[D];新疆农业大学;2001年
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