球孢白僵菌对榕母管蓟马的致病性研究
发布时间：2018-07-29 11:14
【摘要】：榕母管蓟马Gynaikothrips ficorum(Marcha 1908),隶属于缨翅目(Thysanoptera),管蓟马科(phlaeothripidae),母管蓟马属(Gynaikothrips),是榕属(Ficus)植物主要害虫之一。其主要以成虫和若虫聚集取食和产卵危害寄主。本研究筛选对榕母管蓟马具高致病力的白僵菌菌株,研究其生物学特性及其与低毒杀虫剂的相容性和致病力,深入研究在不同温度下白僵菌侵染过程中,榕母管蓟马体内抗氧化酶的应激反应,研究结果如下:1.以浸液法接菌,从12株球孢白僵菌菌株中,筛选出对榕母管蓟马高致病力的菌株。结果表明:以浓度为(1±0.5)×107孢子·mL-1的孢悬液接菌后9 d,菌株Bsx-04对榕母管蓟马致病力相对较强,对成虫和若虫的校正死亡率分别达(100.00±0.00)%和(94.06土1.20)%,LT50分别为(4.03土0.05)d 和(4.71 土0.08)d。Bsx-04 菌株第4天对成虫和若虫的LC50分别4.786× 106孢子·mL-1和1.849×108孢子·mL-1。TDM模型分析,Bsx-04菌株对成虫和若虫致死效应最强的时间都是第5天。2.测定白僵菌Bsx-04菌株在5种常用培养基和不同温度下的菌丝生长量、产孢量和孢子萌发率。结果表明,培养10 d,该菌株在SDAY、PPDA、SMAY3种培养基上菌丝生长显著高于SDA和PDA,5种培养基上的产孢量无显著差异,其中PPDA上菌丝生长和产孢量相对较好。该菌株生长和产孢的最适温度为20℃-25℃,温度35℃不产孢、孢子不萌发,18℃-30℃范围内孢子可萌发,孢子萌发最适温度是25℃。3.研究白僵菌Bsx-04菌株与6种低毒杀虫剂的相容性,测定不同浓度单剂与菌液-杀虫剂混配剂对榕母管蓟马的毒力。结果表明,氟啶虫胺腈与白僵菌Bsx-04孢子萌发抑制率最低,相容性最好。杀虫剂中,乙基多杀菌素和呋虫胺对榕母管蓟马成虫致死效果最好,呋虫胺对其若虫致死效果最好。与亚致死浓度的氰氟虫腙或次亚致死浓度的氟啶虫胺腈混配后,白僵菌Bsx-04对榕母管蓟马成虫的致病力有增效作用,但6种杀虫剂对若虫致病力无增效作用。4.研究榕母管蓟马体内抗氧化酶对白僵菌侵染的应激反应,测定不同温度下,接菌后榕母管蓟马成虫的POD、SOD、CAT 3种抗氧化酶的活性,结果表明:榕母管蓟马成虫受低温或高温胁迫,POD酶的应激反应最强,SOD酶的反应较弱,而CAT酶活性在保持虫体活力较强的条件下,受温度的影响较小。接菌后,相对低温(15℃)或相对高温(35℃)下,3种抗氧化酶活性与对照无显著差异。适温下,3种酶活性均呈先升高后降低的趋势,且温度越有利于白僵菌侵染榕母管蓟马,虫体的抗氧化酶对侵染的应激反应越早,抗氧化酶活性越强。3种抗氧化酶中,SOD酶对白僵菌侵染的应激反应早于POD酶和CAT酶。
[Abstract]:Gynaikothrips ficorum (Marcha 1908, belonging to (Gynaikothrips), of (phlaeothripidae), family (phlaeothripidae), is one of the main pests of (Ficus). It mainly harms the host by gathering adult and nymph to feed and lay eggs. In this study, the biological characteristics, compatibility and pathogenicity of Beauveria bassiana strains with high pathogenicity to Ficus bassiana were studied, and the infection process of Beauveria bassiana at different temperatures was studied. The stress reaction of antioxidant enzymes in Ficus nativa thrips is as follows: 1. 1. From 12 strains of Beauveria bassiana inoculated by dipping solution, the strains with high pathogenicity to Ficus nativa thrips were screened out. The results showed that 9 days after inoculation with spore suspension of (1 卤0.5) 脳 10 ~ 7 spore mL-1, the virulence of strain Bsx-04 to Ficus morifolium thrips was relatively strong. The corrected mortality of adults and nymphs were (100.00 卤0.00)% and (94.06 卤1.20)%, respectively. LT50 was (4.03 卤0.05) d and (4.71 卤0.08) d for adults and nymphs respectively. Analysis of the lethal effects of Bsx-04 on adult and nymph by LC50 4.786 脳 106spore mL-1 and 1.849 脳 108spore mL-1.TDM on the 4th day The most effective time is the fifth day. 2. The hyphae growth, sporulation and spore germination rate of Beauveria bassiana Bsx-04 strain in 5 kinds of common culture medium and at different temperature were measured. The results showed that the hyphal growth of the strain was significantly higher than that of SDA and PDAA on the three media for 10 days, and the hyphae growth and sporulation on PPDA were relatively good. The optimum temperature for the growth and sporulation of the strain was 20 鈩，
本文编号：2152509
[Abstract]:Gynaikothrips ficorum (Marcha 1908, belonging to (Gynaikothrips), of (phlaeothripidae), family (phlaeothripidae), is one of the main pests of (Ficus). It mainly harms the host by gathering adult and nymph to feed and lay eggs. In this study, the biological characteristics, compatibility and pathogenicity of Beauveria bassiana strains with high pathogenicity to Ficus bassiana were studied, and the infection process of Beauveria bassiana at different temperatures was studied. The stress reaction of antioxidant enzymes in Ficus nativa thrips is as follows: 1. 1. From 12 strains of Beauveria bassiana inoculated by dipping solution, the strains with high pathogenicity to Ficus nativa thrips were screened out. The results showed that 9 days after inoculation with spore suspension of (1 卤0.5) 脳 10 ~ 7 spore mL-1, the virulence of strain Bsx-04 to Ficus morifolium thrips was relatively strong. The corrected mortality of adults and nymphs were (100.00 卤0.00)% and (94.06 卤1.20)%, respectively. LT50 was (4.03 卤0.05) d and (4.71 卤0.08) d for adults and nymphs respectively. Analysis of the lethal effects of Bsx-04 on adult and nymph by LC50 4.786 脳 106spore mL-1 and 1.849 脳 108spore mL-1.TDM on the 4th day The most effective time is the fifth day. 2. The hyphae growth, sporulation and spore germination rate of Beauveria bassiana Bsx-04 strain in 5 kinds of common culture medium and at different temperature were measured. The results showed that the hyphal growth of the strain was significantly higher than that of SDA and PDAA on the three media for 10 days, and the hyphae growth and sporulation on PPDA were relatively good. The optimum temperature for the growth and sporulation of the strain was 20 鈩，
本文编号：2152509
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