南平市猪繁殖与呼吸综合征血清学和病原学的监测分析

发布时间：2018-11-02 16:20

【摘要】：猪繁殖与呼吸综合征(英文简称PRRS)又称"蓝耳病",由猪繁殖与呼吸综合征病毒(PRRSV)引起。患病动物以妊娠母猪和仔猪最为常见,且感染后容易发生混合感染,给养猪业带来巨大经济损失,属于我国的一类疫病。为了因地制宜制定猪蓝耳病防控方法,本课题拟对南平市PRRSV发病与强制免疫情况进行系统分析。初步掌握全市高致病性蓝耳病的抗体水平,并结合病原学调查分析深层次因素,为防治猪蓝耳病提供理论依据。在2014-2016年期间,我们在南平市全市范围内60个规模养殖场和90个散养户中随机挑选2040头接种蓝耳疫苗后一个月的健康猪,前腔静脉采集血样,分离血清,采用ELISA检测方法检测猪蓝耳病抗体,其中规模养殖场采样1500头,散养户采样540头。结果显示,全市共有1845份血清样品的PRRSV抗体阳性,合格率为90.4%;其中,规模化养猪场共有1414份血清呈PRRSV抗体阳性,合格率为94.3%;散养式猪场共有431份血清呈PRRSV抗体阳性,合格率为79.8%。从2014-2016年期间,全市每年的猪蓝耳病抗体合格率依次为88.7%、90.4%和92.2%,呈现逐年递增的趋势。同时,我们于2014-2016年期间采集规模养殖场和散养户的猪鼻拭子并提取RNA,采用RT-PCR方法检测其中PRRSV,结果显示,所有待检样品均未检测到PRRSV,这一结果说明所检测的规模养猪场和散养户中暂无猪蓝耳病病毒传播,其所在一定区域内防控工作开展较好。综上所述,南平市全市在2014-2016年期间的猪只PRRSV抗体合格率呈逐年递增趋势,且规模化养殖场的PRRSV抗体合格水平高于散养户。同时,在调查期间,所有检测样品均未检出PRRSV。这提示全市在2014-2016年期间无PRRS疫情,相对于散养户,规模养殖场能够更严格执行免疫计划。
[Abstract]:Porcine reproductive and respiratory syndrome (PRRS), also called blue ear disease, is caused by porcine reproductive and respiratory syndrome virus (PRRSV). Pregnant sows and piglets are the most common diseased animals, and the mixed infection is easy to occur after infection, which brings huge economic losses to the pig industry and belongs to a class of epidemic diseases in China. In order to make the prevention and control method of pig blue ear disease according to local conditions, this paper systematically analyzed the incidence and compulsory immunity of PRRSV in Nanping city. In order to provide theoretical basis for the prevention and treatment of blue ear disease in pigs, the antibody level of high pathogenic blue ear disease in the whole city was preliminarily grasped and the underlying factors were analyzed in combination with etiological investigation. During 2014-2016, we randomly selected 2040 healthy pigs one month after they were vaccinated against blue ear in 60 large-scale farms and 90 free-range households in Nanping City. Blood samples were collected from the anterior vena cava to isolate serum. ELISA detection method was used to detect antibodies to blue ear disease in pigs. Among them, 1500 were sampled in scale farms and 540 in scattered households. The results showed that 1845 serum samples were positive for PRRSV antibody, and the qualified rate was 90.4. Among them, 1414 sera from large-scale pig farms were positive for PRRSV antibody, and the qualified rate was 94.3%. A total of 431 sera were positive for PRRSV antibody, and the qualified rate was 79.8%. From 2014-2016, the qualified rate of antibody against blue ear disease of pigs in the whole city was 88.70.40% and 92.2% respectively, which showed an increasing trend year by year. At the same time, during 2014-2016, we collected pig swabs from large-scale farms and scattered farmers and extracted RNA,. The results of PRRSV, detection by RT-PCR method showed that no PRRSV, was detected in all the samples to be tested. The results showed that there was no transmission of blue ear disease virus in pig farms and free-range farms, and the prevention and control work was well carried out in a certain area. To sum up, the qualified rate of PRRSV antibody of pigs in Nanping city from 2014-2016 is increasing year by year, and the qualified level of PRRSV antibody in large-scale breeding farm is higher than that in retail farmers. At the same time, no PRRSV. was detected in all samples during the investigation. This suggests that there will be no PRRS outbreak in the city between 2014-2016 and that scale farms will be able to enforce immunization programs more strictly than free-range farmers.
【学位授予单位】：福建农林大学
【学位级别】：硕士
【学位授予年份】：2017
【分类号】：S858.28

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