盐胁迫下景天植物的超微弱发光及其激发机制

发布时间：2018-11-12 18:37

【摘要】：本试验以景天属植物德景天为试验材料进行盐逆胁迫,并在盐胁迫下进行活性氧自由基诱发和活性氧自由基清除处理。通过研究景天属植物在盐逆境胁迫下及活性氧自由基诱发和清除情况下受胁迫程度、超微弱发光(UWL)、活性氧系统、能量水平、光合作用等的变化,探讨盐逆境胁迫下景天植物UWL的变化及侧重于活性氧方面的产生机制。本研究主要结果如下:1.试验对德景天叶片在NaCl盐胁迫下的UWL变化、生长活力、光合作用、活性氧系统、能量水平进行了研究。结果表明:盐胁迫下,德景天叶片的UWL强度下降,相对含水量下降,根系活力降低,束缚水与自由水的比值上升,相对含水量与UWL之间呈高度正相关,差异性显著,束缚水与自由水比值与UWL之间呈高度负相关,差异性显著;Pn、Tr、Gs、Ci、Chla含量、Chlb含量、Chl总含量下降,光合能力减弱,UWL与Pn、Tr、Gs、Ci、Chla含量、Chlb含量、Chl总含量呈高度正相关,差异性显著;O2-产生速率、MDA含量、相对电导率上升,UWL与O2-产生速率、MDA含量、相对电导率呈高度负相关,差异性显著;ATP含量呈下降趋势,能荷值下降,ADP和AMP含量整体上升,ATP、能荷值均与UWL呈高度正相关,差异性极显著。反映了盐胁迫程度。初步表明光合作用,能量相关与UWL产生有关,活性氧不是产生UWL的直接来源。2.试验进一步对德景天叶片在施加过氧化氢(H2O2)和苯甲酸钠调控盐胁迫下的UWL变化、生长活力、光合作用、活性氧系统、能量水平进行了研究。结果表明:在盐胁迫下,NaCl处理(对照)、NaCl+H2O2处理和NaCl+苯甲酸钠处理的德景天叶片的UWL强度下降,相对含水量下降,根系活力降低,束缚水与自由水比值上升,相对含水量与UWL之间呈高度正相关,差异性显著,束缚水与自由水比值与UWL之间呈高度负相关,差异性显著;Pn、Tr、Gs、Ci、Chla含量、Chlb含量、Chl含量下降,光合能力减弱,UWL与Pn、Tr、Gs、Ci、Chla含量、Chlb含量、Chl含量呈正相关,差异性显著;O2-产生速率、MDA含量、相对电导率上升,UWL与O2-产生速率、MDA含量、相对电导率呈负相关,差异性显著;ATP含量下降趋势,能荷值下降,ADP和AMP含量整体上升,ATP、能荷值均与UWL呈高度正相关,差异性极显著。整个胁迫过程中,H2O2处理加剧了相对含水量、Pn、Tr、Gs、Ci、Chla含量、Chlb含量、Chl总含量以及ATP含量的降低,加剧了束缚水与自由水比值、O2-产生速率、MDA含量、相对电导率的上升,同时加剧了 UWL的下降;苯甲酸钠处理减缓了相对含水量、Pn、Tr、Gs、Ci、Chla含量、Chlb含量、Chl总含量以及ATP含量的降低,减缓了束缚水与自由水比值、O2-产生速率、MDA含量、相对电导率的上升,同时减缓了 UWL的下降。进一步说明光合作用,能量相关与UWL产生有关,活性氧是通过间接作用影响UWL。
[Abstract]:In this experiment, Sedum was used as the experimental material to carry out salt reverse stress, and under salt stress, reactive oxygen free radical induction and scavenging of active oxygen free radical were carried out. The changes of reactive oxygen species system, energy level and photosynthesis of ultraweak luminescent (UWL), under salt stress and free radical scavenging of reactive oxygen species were studied. To study the changes of UWL in Sedum plants under salt stress and the mechanism of production of active oxygen species (Ros). The main results of this study are as follows: 1. The changes of UWL, growth vigor, photosynthesis, reactive oxygen species system and energy level in leaves of Sedum dejingensis under NaCl salt stress were studied. The results showed that under salt stress, the UWL intensity, relative water content, root activity and the ratio of bound water to free water were decreased, and the relative water content was positively correlated with UWL, and the difference was significant. The ratio of bound water to free water was negatively correlated with UWL, and the difference was significant. Pn,Tr,Gs,Ci,Chla content, Chlb content, total Chl content decreased, photosynthetic ability decreased, UWL and Pn,Tr,Gs,Ci,Chla content, Chlb content, Chl content were highly positive correlation, the difference was significant. O _ 2-production rate, MDA content, relative conductivity increased, UWL and O _ 2- production rate, MDA content, relative conductivity were highly negative correlation, the difference was significant. ATP content decreased, energy charge value decreased, ADP and AMP content increased as a whole. ATP, energy charge value was highly positively correlated with UWL, and the difference was very significant. It reflects the degree of salt stress. The results showed that photosynthesis and energy correlation were related to UWL production, and reactive oxygen species were not the direct source of UWL production. 2. The effects of hydrogen peroxide (H2O2) and sodium benzoate on the changes of UWL, growth activity, photosynthesis, reactive oxygen system and energy level in leaves of Sedum were studied. The results showed that under salt stress, the UWL intensity, relative water content, root activity and ratio of bound water to free water of leaves treated with NaCl (control), NaCl H2O2 and NaCl benzoate) decreased, relative water content decreased, and the ratio of bound water to free water increased. The relative water content was highly positively correlated with UWL and the difference was significant. The ratio of bound water to free water was highly negatively correlated with UWL, and the difference was significant. Pn,Tr,Gs,Ci,Chla content, Chlb content, Chl content decreased, photosynthetic ability decreased, UWL and Pn,Tr,Gs,Ci,Chla content, Chlb content, Chl content were positively correlated, the difference was significant. O _ 2-production rate, MDA content, relative conductivity increased, UWL and O _ 2- production rate, MDA content, relative conductivity were negatively correlated, the difference was significant. ATP content decreased, energy charge value decreased, ADP and AMP content increased as a whole. ATP, energy charge value was highly positively correlated with UWL, and the difference was very significant. During the whole stress process, the relative water content, Pn,Tr,Gs,Ci,Chla content, Chlb content, total Chl content and ATP content decreased, the ratio of bound water to free water, O _ 2-production rate, MDA content were increased by H2O2 treatment. The increase of relative conductivity and the decrease of UWL; Sodium benzoate treatment slowed down the decrease of relative water content, Pn,Tr,Gs,Ci,Chla content, Chlb content, total Chl content and ATP content, slowed down the ratio of bound water to free water, O _ 2-producing rate, MDA content and relative conductivity. At the same time, the decline of UWL was slowed down. It is further explained that photosynthesis and energy correlation are related to the production of UWL, and reactive oxygen species affect UWL. indirectly.
【学位授予单位】：内蒙古农业大学
【学位级别】：硕士
【学位授予年份】：2017
【分类号】：S682.33

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